KEGG   ENZYME: 1.14.14.162Help
Entry
EC 1.14.14.162              Enzyme                                 

Name
flavanone 2-hydroxylase;
CYP93G2 (gene name);
CYP93B1 (gene name);
(2S)-flavanone 2-hydroxylase;
licodione synthase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
flavanone,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (2-hydroxylating)
Reaction(IUBMB)
a flavanone + [reduced NADPH---hemoprotein reductase] + O2 = a 2-hydroxyflavanone + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R12218]
Reaction(KEGG)
R12218;
(other) R07198
Show
Substrate
flavanone [CPD:C00766];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007]
Product
2-hydroxyflavanone [CPD:C22005];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001]
Comment
A cytochrome P-450 (heme thiolate) plant enzyme that catalyses the 2-hydroxylation of multiple flavanones such as (2S)-naringenin, (2S)-eriodictyol, (2S)-pinocembrin, and (2S)-liquiritigenin. The products are meta-stable and exist in an equilibrium with open forms such as 1-(4-hydroxyphenyl)-3-(2,4,6-trihydroxyphenyl)propane-1,3-dione.
History
EC 1.14.14.162 created 2018. EC 1.14.14.140 created 2004 as EC 1.14.13.87, transferred 2018 to EC 1.14.14.140, transferred 2018 to EC 1.14.14.162
Orthology
K13406  flavanone 2-hydroxylase
K23135  flavanone 2-hydroxylase
Genes
OSA: 4339836
DOSA: Os06t0102100-01(Os06g0102100)
OBR: 102709090
BDI: 100828788
ATS: 109777288(LOC109777288)
SBI: 8080808
ZMA: 103639121
SITA: 101764350
Taxonomy
Reference
1  [PMID:12232298]
  Authors
Otani K, Takahashi T, Furuya T, Ayabe S.
  Title
Licodione Synthase, a Cytochrome P450 Monooxygenase Catalyzing 2-Hydroxylation of 5-Deoxyflavanone, in Cultured Glycyrrhiza echinata L. Cells.
  Journal
Plant Physiol 105:1427-1432 (1994)
Reference
2  [PMID:9708921]
  Authors
Akashi T, Aoki T, Ayabe S.
  Title
Identification of a cytochrome P450 cDNA encoding (2S)-flavanone 2-hydroxylase of licorice (Glycyrrhiza echinata L.; Fabaceae) which represents licodione synthase and flavone synthase II.
  Journal
FEBS Lett 431:287-90 (1998)
DOI:10.1016/S0014-5793(98)00781-9
  Sequence
Reference
3  [PMID:20647377]
  Authors
Du Y, Chu H, Chu IK, Lo C
  Title
CYP93G2 is a flavanone 2-hydroxylase required for C-glycosylflavone biosynthesis  in rice.
  Journal
Plant Physiol 154:324-33 (2010)
DOI:10.1104/pp.110.161042
  Sequence
[osa:4339836]
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.162
IUBMB Enzyme Nomenclature: 1.14.14.162
ExPASy - ENZYME nomenclature database: 1.14.14.162
BRENDA, the Enzyme Database: 1.14.14.162

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