KEGG   ENZYME: 1.14.14.162
Entry
EC 1.14.14.162              Enzyme                                 

Name
flavanone 2-hydroxylase;
CYP93G2 (gene name);
CYP93B1 (gene name);
(2S)-flavanone 2-hydroxylase;
licodione synthase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
flavanone,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (2-hydroxylating)
Reaction(IUBMB)
a flavanone + [reduced NADPH---hemoprotein reductase] + O2 = a 2-hydroxyflavanone + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R12218]
Reaction(KEGG)
R12218;
(other) R07198
Substrate
flavanone [CPD:C00766];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007]
Product
2-hydroxyflavanone [CPD:C22005];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001]
Comment
A cytochrome P-450 (heme thiolate) plant enzyme that catalyses the 2-hydroxylation of multiple flavanones such as (2S)-naringenin, (2S)-eriodictyol, (2S)-pinocembrin, and (2S)-liquiritigenin. The products are meta-stable and exist in an equilibrium with open forms such as 1-(4-hydroxyphenyl)-3-(2,4,6-trihydroxyphenyl)propane-1,3-dione.
History
EC 1.14.14.162 created 2018. EC 1.14.14.140 created 2004 as EC 1.14.13.87, transferred 2018 to EC 1.14.14.140, transferred 2018 to EC 1.14.14.162
Orthology
K13406  flavanone 2-hydroxylase
K23135  flavanone 2-hydroxylase
Genes
OSA: 4339836
DOSA: Os06t0102100-01(Os06g0102100)
OBR: 102709090
BDI: 100828788
ATS: 109777288
SBI: 8054481 8080808
ZMA: 103639121 109944638
SITA: 101764350
Reference
1  [PMID:12232298]
  Authors
Otani K, Takahashi T, Furuya T, Ayabe S.
  Title
Licodione Synthase, a Cytochrome P450 Monooxygenase Catalyzing 2-Hydroxylation of 5-Deoxyflavanone, in Cultured Glycyrrhiza echinata L. Cells.
  Journal
Plant Physiol 105:1427-1432 (1994)
DOI:10.1104/pp.105.4.1427
Reference
2  [PMID:9708921]
  Authors
Akashi T, Aoki T, Ayabe S
  Title
Identification of a cytochrome P450 cDNA encoding (2S)-flavanone 2-hydroxylase of licorice (Glycyrrhiza echinata L.; Fabaceae) which represents licodione synthase and flavone synthase II.
  Journal
FEBS Lett 431:287-90 (1998)
DOI:10.1016/S0014-5793(98)00781-9
  Sequence
Reference
3  [PMID:20647377]
  Authors
Du Y, Chu H, Chu IK, Lo C
  Title
CYP93G2 is a flavanone 2-hydroxylase required for C-glycosylflavone biosynthesis in rice.
  Journal
Plant Physiol 154:324-33 (2010)
DOI:10.1104/pp.110.161042
  Sequence
[osa:4339836]
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.162
IUBMB Enzyme Nomenclature: 1.14.14.162
ExPASy - ENZYME nomenclature database: 1.14.14.162
BRENDA, the Enzyme Database: 1.14.14.162

  All links  
Chemical substance (8)   
   KEGG COMPOUND (8)   
Chemical reaction (4)   
   KEGG REACTION (2)   
   KEGG RCLASS (2)   
Gene (13)   
   KEGG ORTHOLOGY (2)   
   KEGG GENES (11)   
Protein sequence (4)   
   UniProt (2)   
   SWISS-PROT (2)   
Protein domain (4)   
   InterPro (4)   
All databases (33)   

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