KEGG   ENZYME: 1.14.14.162
Entry
EC 1.14.14.162              Enzyme                                 
Name
flavanone 2-hydroxylase;
CYP93G2 (gene name);
CYP93B1 (gene name);
(2S)-flavanone 2-hydroxylase;
licodione synthase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
flavanone,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (2-hydroxylating)
Reaction(IUBMB)
a flavanone + [reduced NADPH---hemoprotein reductase] + O2 = a 2-hydroxyflavanone + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R12218]
Reaction(KEGG)
R12218;
(other) R07198
Substrate
flavanone [CPD:C00766];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007]
Product
2-hydroxyflavanone [CPD:C22005];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001]
Comment
A cytochrome P-450 (heme thiolate) plant enzyme that catalyses the 2-hydroxylation of multiple flavanones such as (2S)-naringenin, (2S)-eriodictyol, (2S)-pinocembrin, and (2S)-liquiritigenin. The products are meta-stable and exist in an equilibrium with open forms such as 1-(4-hydroxyphenyl)-3-(2,4,6-trihydroxyphenyl)propane-1,3-dione.
History
EC 1.14.14.162 created 2018. EC 1.14.14.140 created 2004 as EC 1.14.13.87, transferred 2018 to EC 1.14.14.140, transferred 2018 to EC 1.14.14.162
Orthology
K13406  flavanone 2-hydroxylase
K23135  flavanone 2-hydroxylase
Genes
OSA: 4339836
DOSA: Os06g0102100
OBR: 102709090
OGL: 127776705
BDI: 100828788
ATS: 109775389 109777288 109787084
TDC: 119329547 119331174 119338203 119341127
TAES: 123146873 123147834 123159122 123161472 123166592 123169858
TUA: 125521719 125521845 125524944
LRD: 124683469
SBI: 8054481 8080808
ZMA: 103639121 109944638
MFLO: 136473528 136497297 136499024 136501989 136530447 136544957 136544958
SITA: 101764350
SVS: 117843456
PHAI: 112879889
PAUA: 133931056
 » show all
Reference
1  [PMID:12232298]
  Authors
Otani K, Takahashi T, Furuya T, Ayabe S.
  Title
Licodione Synthase, a Cytochrome P450 Monooxygenase Catalyzing 2-Hydroxylation of 5-Deoxyflavanone, in Cultured Glycyrrhiza echinata L. Cells.
  Journal
Plant Physiol 105:1427-1432 (1994)
DOI:10.1104/pp.105.4.1427
Reference
2  [PMID:9708921]
  Authors
Akashi T, Aoki T, Ayabe S
  Title
Identification of a cytochrome P450 cDNA encoding (2S)-flavanone 2-hydroxylase of licorice (Glycyrrhiza echinata L.; Fabaceae) which represents licodione synthase and flavone synthase II.
  Journal
FEBS Lett 431:287-90 (1998)
DOI:10.1016/S0014-5793(98)00781-9
  Sequence
[ag:BAA22423]
Reference
3  [PMID:20647377]
  Authors
Du Y, Chu H, Chu IK, Lo C
  Title
CYP93G2 is a flavanone 2-hydroxylase required for C-glycosylflavone biosynthesis in rice.
  Journal
Plant Physiol 154:324-33 (2010)
DOI:10.1104/pp.110.161042
  Sequence
[osa:4339836]
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.162
IUBMB Enzyme Nomenclature: 1.14.14.162
ExPASy - ENZYME nomenclature database: 1.14.14.162
BRENDA, the Enzyme Database: 1.14.14.162

  All links  
Protein sequence (4)   
   UniProt (2)   
   SWISS-PROT (2)   
Protein domain (4)   
   InterPro (4)   
All databases (8)   

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