| Entry |
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| Name |
(S)-1-hydroxy-N-methylcanadine 13-hydroxylase;
CYP82X2 (gene name)
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| Class |
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
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| Sysname |
(S)-1-hydroxy-N-methylcanadine,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (13-hydroxylating)
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| Reaction(IUBMB) |
(S)-1-hydroxy-N-methylcanadine + [reduced NADPH---hemoprotein reductase] + O2 = (13S,14R)-1,13-dihydroxy-N-methylcanadine + [oxidized NADPH---hemoprotein reductase] + H2O [RN: R11688]
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| Reaction(KEGG) |
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| Substrate |
(S)-1-hydroxy-N-methylcanadine [CPD: C21586];
[reduced NADPH---hemoprotein reductase] [CPD: C03024];
O2 [CPD: C00007]
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| Product |
(13S,14R)-1,13-dihydroxy-N-methylcanadine [CPD: C21587];
[oxidized NADPH---hemoprotein reductase] [CPD: C03161];
H2O [CPD: C00001]
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| Comment |
The enzyme, characterized from the plant Papaver somniferum (opium poppy), participates in the biosynthesis of the isoquinoline alkaloid noscapine.
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| History |
EC 1.14.14.163 created 2018
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| Pathway |
| ec00950 | Isoquinoline alkaloid biosynthesis |
| ec01110 | Biosynthesis of secondary metabolites |
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| Orthology |
| K22094 | (S)-1-hydroxy-N-methylcanadine 13-hydroxylase |
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| Genes |
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| Reference |
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| Authors |
Dang TT, Chen X, Facchini PJ. |
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| Title |
Acetylation serves as a protective group in noscapine biosynthesis in opium poppy. |
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| Journal |
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| Reference |
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| Authors |
Li Y, Smolke CD |
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| Title |
Engineering biosynthesis of the anticancer alkaloid noscapine in yeast. |
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| Journal |
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| Reference |
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| Authors |
Li Y, Li S, Thodey K, Trenchard I, Cravens A, Smolke CD |
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| Title |
Complete biosynthesis of noscapine and halogenated alkaloids in yeast. |
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| Journal |
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| Other DBs |
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