KEGG   ENZYME: 1.14.14.165
Entry
EC 1.14.14.165              Enzyme                                 

Name
indole-3-carbonyl nitrile 4-hydroxylase;
CYP82C2
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
indole-3-carbonyl nitrile,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (4-hydroxylating)
Reaction(IUBMB)
indole-3-carbonyl nitrile + [reduced NADPH---hemoprotein reductase] + O2 = 4-hydroxyindole-3-carbonyl nitrile + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R12232]
Reaction(KEGG)
R12232
Substrate
indole-3-carbonyl nitrile [CPD:C22015];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007]
Product
4-hydroxyindole-3-carbonyl nitrile [CPD:C22016];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001]
Comment
A cytochrome P-450 (heme-thiolate) protein characterized from the plant Arabidopsis thaliana. Involved in biosynthesis of small cyanogenic compounds that take part in pathogen defense. The enzyme also catalyses the 5-hydroxylation of xanthotoxin [1].
History
EC 1.14.14.165 created 2018
Orthology
K23137  indole-3-carbonyl nitrile 4-hydroxylase
Genes
ATH: AT4G31970(CYP82C2)
ALY: 9305373
Reference
1  [PMID:18291319]
  Authors
Kruse T, Ho K, Yoo HD, Johnson T, Hippely M, Park JH, Flavell R, Bobzin S
  Title
In planta biocatalysis screen of P450s identifies 8-methoxypsoralen as a substrate for the CYP82C subfamily, yielding original chemical structures.
  Journal
Chem Biol 15:149-56 (2008)
DOI:10.1016/j.chembiol.2008.01.008
  Sequence
[ath:AT4G31970]
Reference
2  [PMID:26352477]
  Authors
Rajniak J, Barco B, Clay NK, Sattely ES
  Title
A new cyanogenic metabolite in Arabidopsis required for inducible pathogen defence.
  Journal
Nature 525:376-9 (2015)
DOI:10.1038/nature14907
  Sequence
[ath:AT4G31970]
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.165
IUBMB Enzyme Nomenclature: 1.14.14.165
ExPASy - ENZYME nomenclature database: 1.14.14.165
BRENDA, the Enzyme Database: 1.14.14.165

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