KEGG   ENZYME: 1.14.14.171
Entry
EC 1.14.14.171              Enzyme                                 

Name
beta-amyrin 16alpha-hydroxylase;
CYP87D16
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
beta-amyrin,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (16alpha-hydroxylating)
Reaction(IUBMB)
beta-amyrin + [reduced NADPH---hemoprotein reductase] + O2 = 16alpha-hydroxy-beta-amyrin + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R12367]
Reaction(KEGG)
R12367
Substrate
beta-amyrin [CPD:C08616];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007]
Product
16alpha-hydroxy-beta-amyrin [CPD:C22113];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001]
Comment
A cytochrome P-450 (heme-thiolate) protein isolated from the plant Maesa lanceolata (false assegai). Involved in the biosynthesis of maesasaponins. It also acts on some derivatives of beta-amyrin such as erythrodiol or oleanolic acid.
History
EC 1.14.14.171 created 2019
Orthology
K20622  beta-amyrin 16alpha-hydroxylase
Reference
1  [PMID:24434554]
  Authors
Moses T, Pollier J, Almagro L, Buyst D, Van Montagu M, Pedreno MA, Martins JC, Thevelein JM, Goossens A
  Title
Combinatorial biosynthesis of sapogenins and saponins in Saccharomyces cerevisiae using a C-16alpha hydroxylase from Bupleurum falcatum.
  Journal
Proc Natl Acad Sci U S A 111:1634-9 (2014)
DOI:10.1073/pnas.1323369111
  Sequence
Reference
2  [PMID:25578277]
  Authors
Moses T, Pollier J, Faizal A, Apers S, Pieters L, Thevelein JM, Geelen D, Goossens A
  Title
Unraveling the triterpenoid saponin biosynthesis of the African shrub Maesa lanceolata.
  Journal
Mol Plant 8:122-35 (2015)
DOI:10.1016/j.molp.2014.11.004
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.171
IUBMB Enzyme Nomenclature: 1.14.14.171
ExPASy - ENZYME nomenclature database: 1.14.14.171
BRENDA, the Enzyme Database: 1.14.14.171

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