Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
The enzyme, characterized from a number of bacterial species, participates in the degradation of 2,4,6-trichlorophenol, a compound that has been used for decades as a wood preservative. The enzyme is a multifunctional flavin-dependent monooxygenase that displaces two chlorine atoms by attacking two different positions in the substrate. Each reaction catalysed by the enzyme displaces a single chlorine and results in formation of a dione, which must be reduced by FADH2 before the monooxygenase could catalyse the second reaction. The large amount of FADH2 that is required is generated by a dedicated flavin reductase (TcpB). cf. EC 126.96.36.199, 3,5,6-trichloropyridin-2-ol monooxygenase.