KEGG   ENZYME: 1.14.14.179
Entry
EC 1.14.14.179              Enzyme                                 
Name
brassinosteroid 6-oxygenase;
CYP85A1 (gene name);
CYP85A2 (gene name);
brassinosteroid 6-oxidase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
6-deoxocastasterone,NADPH---hemoprotein reductase:oxygen 6-oxidoreductase (castasterone-forming)
Reaction(IUBMB)
6-deoxocastasterone + 2 O2 + 2 [reduced NADPH---hemoprotein reductase] = castasterone + 3 H2O + 2 [oxidized NADPH---hemoprotein reductase] (overall reaction) [RN:R10669];
(1a) 6-deoxocastasterone + O2 + [reduced NADPH---hemoprotein reductase] = 6alpha-hydroxy-6-deoxocastasterone + H2O + [oxidized NADPH---hemoprotein reductase] [RN:R07450];
(1b) 6alpha-hydroxy-6-deoxocastasterone + O2 + [reduced NADPH---hemoprotein reductase] = castasterone + 2 H2O + [oxidized NADPH---hemoprotein reductase] [RN:R07451]
Reaction(KEGG)
Substrate
6-deoxocastasterone [CPD:C15802];
O2 [CPD:C00007];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
6alpha-hydroxy-6-deoxocastasterone
Product
castasterone [CPD:C15794];
H2O [CPD:C00001];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
6alpha-hydroxy-6-deoxocastasterone
Comment
This cytochrome P-450 (heme thiolate) plant enzyme catalyses the C-6 hydoxylation of several brassinosteroid biosynthesis intermediates, and the further oxidation of the hydroxyl group to an oxo group. Substrates include 6-deoxocastasterone, 6-deoxotyphasterol, 3-dehydro-6-deoxoteasterone, and 6-deoxoteasterone. The CYP85A2 isozyme of Arabidopsis thaliana (but not the CYP85A1 isozyme) also catalyses the activity of EC 1.14.14.180, brassinolide synthase.
History
EC 1.14.14.179 created 2022
Pathway
ec00905  Brassinosteroid biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K09590  brassinosteroid 6-oxygenase
K12640  brassinosteroid 6-oxygenase / brassinolide synthase
K25959  brassinosteroid 6-oxygenase / brassinolide synthase
Genes
ATHAT3G30180(BR6OX2) AT5G38970(BR6OX1)
ALY9306879 9311596
CRB17880458 17885676
CSAT104710811 104719559 104724260 104732939 104780197 104790595
EUSEUTSA_v10004156mg EUTSA_v10027742mg
BRP103837220 103854484 103864167 103875063
BNA106346024 106349108 106363995 106397123 106400040 106410432 106410433 106411244 106449738
BOE106301844 106316572 106339725
RSZ108807315 108825972 108837548 108849348
THJ104805522 104822777 104823884
CPAP110820131 110823995
CIT102613870 102627864
CICCICLE_v10011662mg CICLE_v10031449mg
PVY116109710 116132910
MINC123198264 123213958 123216775 123226215
TCC18601740 18610219
GRA105781807 105789387 105801672
GHI107894854 107910165 107919617 107954898 121219372 121232120
GAB108453607 108463311 108482799
DZI111283830 111306268 111313564 111317590
EGR104419439 104422041 104422043 104422045 104429916 104429918
GMX100779967 100785414 100805958 100818004 100818290
GSJ114372680 114381879 114395015 114399240 114423881
PVUPHAVU_003G040300g PHAVU_004G041700g PHAVU_008G028800g
VRA106753400 106758156 106779292
VAR108326910 108335521 108344744
VUN114166182 114181354 114183716
CCAJ109796264 109797613 109813276 109818989
APRC113861763 113867653 113869100 113869572
MTRMTR_3g007320 MTR_6g015910 MTR_7g016040
CAM101509866 101510185 101510551
LJALj0g3v0092539.1(Lj0g3v0092539.1) Lj0g3v0228369.1(Lj0g3v0228369.1) Lj2g3v1241200.1(Lj2g3v1241200.1) Lj2g3v1241200.2(Lj2g3v1241200.2) Lj2g3v1241200.3(Lj2g3v1241200.3) Lj2g3v1241200.4(Lj2g3v1241200.4)
ADU107471377 107471379 107486576
AIP107623811 107637361
AHF112716544 112716545 112744628 112784627 112784628 112797930
LANG109330371 109335133 109347392 109359433 109359685 109360156
FVE101313910
RCN112190949
PPER18782277
PMUM103329572
PAVI110759051
PDUL117623030
MDM103404651 103442442
PXB103926586 103962743
ZJU107429039
MNT21401153 21409111
CSV101204494 101206858
CMO103495886 103498545
BHJ120079745
MCHA111006661
CMAX111487713 111489110
CMOS111445417 111455792
CPEP111788371 111808466
RCU8277307
JCU105630569 105646746
HBR110648160 110655288 110672384 110672390
MESC110609099 110615056 110619853
POP18107339 7468141 7480593
PEU105113937 105119329 105123253
PALZ118042705 118043350 118055061
JRE108987930 108989489
QSU111985795 112004758 112004759 112006726
QLO115976259 115981444 115981445
TWL119993370 120011335 120017232
VVI100232983(BR6OX1) 100257818 100262943
VRI117923784 117925175 117929660
SLY100037489(CYP85A1) 100136888(CYP85A3)
SPEN107009077 107010718
SOT102599687 102603315
SSTN125848344 125849224
CANN107860427 107861006
NTA107760973 107776096 107780292 107789559
NSY104211653 104214321
NTO104092995 104103228
NAU109212730 109222927
INI109153620 109173064 109180625 109189067
ITR115999955 116013189 116013963 116019775
SIND105156751 105177542
OEU111374568 111381944 111409964
EGT105952596 105971548
SSPL121754265 121755396 121767430 121770076
HAN110911035 110921448
ECAD122579005 122589730
LSV111876869
CCAV112513235 112515661
DCR108193941 108194607 108213230
CSIN114256381 114306437
BVG104906946
SOE110793171
CQI110703563 110705784
NNU104597895 104599689 104609612
MING122075756 122078986
TSS122655820 122663736
PSOM113275767 113281843
NCOL116258091 116258773
OSA4333399
DOSAOs03t0602300-01(Os03g0602300)
OBR102700319
BDI100837653
ATS109777766 109777767 109777768 109777769 109777770
TDC119352698 119352699 119358574 119358575 119360155 119361707 119361709 119366782 119366783
TAES123038724 123039915 123043102 123043103 123048178 123048179 123050998 123051000 123051001 123184190 123186954 123186955 123186956
TUA125534977 125540566 125540569 125540570
SBI8062533
ZMA100193331
SITA101770408
SVS117838234
PVIR120650403 120692586
PHAI112875680
PDA103696100 103704209 103704493 103722877
EGU105046675 105056073 105057148 105058109
MUS103975616 103982137 103986539 103996475
DCT110095594 110112633
PEQ110030284 110033615 110034826 110039402
AOF109822473 109825906 109845117 109846361
ATR18446218 18446219
PPP112292180
 » show all
Reference
1  [PMID:11402205]
  Authors
Shimada Y, Fujioka S, Miyauchi N, Kushiro M, Takatsuto S, Nomura T, Yokota T, Kamiya Y, Bishop GJ, Yoshida S
  Title
Brassinosteroid-6-oxidases from Arabidopsis and tomato catalyze multiple C-6 oxidations in brassinosteroid biosynthesis.
  Journal
Plant Physiol 126:770-9 (2001)
DOI:10.1104/pp.126.2.770
  Sequence
[ath:AT5G38970] [sly:100037489]
Reference
2  [PMID:21364326]
  Authors
Perez-Espana VH, Sanchez-Leon N, Vielle-Calzada JP.
  Title
CYP85A1 is required for the initiation of female gametogenesis in Arabidopsis thaliana.
  Journal
Plant Signal Behav 6:321-6 (2011)
DOI:10.4161/psb.6.3.13206
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.179
IUBMB Enzyme Nomenclature: 1.14.14.179
ExPASy - ENZYME nomenclature database: 1.14.14.179
BRENDA, the Enzyme Database: 1.14.14.179

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