KEGG   ENZYME: 1.14.14.185
Entry
EC 1.14.14.185              Enzyme                                 
Name
taxane 9alpha-hydroxylase;
taxoid 9alpha hydroxylase;
CYP725A22;
T9alphaOH
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
5,20-epoxytax-11-en-4alpha-ol,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (9alpha-hydroxylating)
Reaction(IUBMB)
5,20-epoxytax-11-en-4alpha-ol + [reduced NADPH---hemoprotein reductase] + O2 = 5,20-epoxytax-11-ene-4alpha,9alpha-diol + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R13290]
Reaction(KEGG)
R13290;
(other) R13550
Substrate
5,20-epoxytax-11-en-4alpha-ol [CPD:C22889];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007]
Product
5,20-epoxytax-11-ene-4alpha,9alpha-diol [CPD:C22891];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001]
Comment
The enzyme is active in the biosynthetic pathway of paclitaxel (Taxol) in Taxus species (yew).
History
EC 1.14.14.185 created 2024
Orthology
K27503  taxane 9alpha-hydroxylase
Reference
1  [PMID:37897038]
  Authors
Zhang Y, Wiese L, Fang H, Alseekh S, Perez de Souza L, Scossa F, Molloy J, Christmann M, Fernie AR.
  Title
Synthetic biology identifies the minimal gene set required for paclitaxel biosynthesis in a plant chassis.
  Journal
Mol Plant 16:1951-1961 (2023)
DOI:10.1016/j.molp.2023.10.016
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.185
IUBMB Enzyme Nomenclature: 1.14.14.185
ExPASy - ENZYME nomenclature database: 1.14.14.185
BRENDA, the Enzyme Database: 1.14.14.185

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