KEGG   ENZYME: 1.14.14.19Help
Entry
EC 1.14.14.19               Enzyme                                 

Name
steroid 17alpha-monooxygenase;
steroid 17alpha-hydroxylase;
cytochrome P-450 17alpha;
cytochrome P-450 (P-450 17alpha,lyase);
17alpha-hydroxylase-C17,20 lyase;
CYP17;
CYP17A1 (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
steroid,NADPH---hemoprotein reductase:oxygen oxidoreductase (17alpha-hydroxylating)
Reaction(IUBMB)
a C21-steroid + [reduced NADPH---hemoprotein reductase] + O2 = a 17alpha-hydroxy-C21-steroid + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R02131]
Reaction(KEGG)
Substrate
C21-steroid;
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007]
Product
17alpha-hydroxy-C21-steroid;
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001]
Comment
Requires NADPH and EC 1.6.2.4, NADPH---hemoprotein reductase. A microsomal hemeprotein that catalyses two independent reactions at the same active site - the 17alpha-hydroxylation of pregnenolone and progesterone, which is part of glucocorticoid hormones biosynthesis, and the conversion of the 17alpha-hydroxylated products via a 17,20-lyase reaction to form androstenedione and dehydroepiandrosterone, leading to sex hormone biosynthesis (EC 1.14.14.32, 17alpha-hydroxyprogesterone deacetylase). The ratio of the 17alpha-hydroxylase and 17,20-lyase activities is an important factor in determining the directions of steroid hormone biosynthesis towards biosynthesis of glucocorticoid or sex hormones.
History
EC 1.14.14.19 created 1961 as EC 1.99.1.9, transferred 1965 to EC 1.14.1.7, transferred 1972 to EC 1.14.99.9, modified 2013, transferred 2015 to EC 1.14.14.19
Pathway
ec00140  Steroid hormone biosynthesis
ec01100  Metabolic pathways
Orthology
K00512  steroid 17alpha-monooxygenase / 17alpha-hydroxyprogesterone deacetylase
Genes
HSA: 1586(CYP17A1)
PTR: 450141(CYP17A1)
PPS: 100989182
GGO: 101152080
PON: 100439753
NLE: 100596228
MCC: 678693(CYP17A1)
MCF: 102133235(CYP17)
CSAB: 103216445(CYP17A1)
RRO: 104656530
RBB: 108515083
CJC: 100400572(CYP17A1)
SBQ: 101048584
MMU: 13074(Cyp17a1)
MCAL: 110285327
MPAH: 110334801
RNO: 25146(Cyp17a1)
MUN: 110556807
CGE: 100758683
NGI: 103744072
HGL: 101726496
CCAN: 109691327
OCU: 100346394
TUP: 102472543
CFA: 477807(CYP17A1)
VVP: 112917782
AML: 100476785
UMR: 103681600
UAH: 113251880
ORO: 101384623
FCA: 493967(CYP17A1)
PTG: 102966057
PPAD: 109272832
AJU: 106966901
BTA: 112441470 281739(CYP17A1)
CHX: 102182376 102182663(CYP17)
OAS: 105601846(CYP17) 493968(CYP17A1)
SSC: 403330(CYP17A1)
CFR: 102519078
CDK: 105095024
BACU: 103007900
LVE: 103081477
OOR: 101283719
DLE: 111172355
PCAD: 102989171
ECB: 100034232(CYP17A1)
EPZ: 103558137
EAI: 106838161
MYB: 102259343
MYD: 102761363
MNA: 107542528
HAI: 109379659
DRO: 112300189
PALE: 102884618
RAY: 107515566
MJV: 108410298
LAV: 100660963
TMU: 101354636
MDO: 100028896
SHR: 100915970
PCW: 110194957
OAA: 100080906
GGA: 425056(CYP17A1)
MGP: 100540331
CJO: 107316091(P-450c17)
NMEL: 110399428
APLA: 101792920
ACYG: 106031836
TGU: 100218933
LSR: 110473583
SCAN: 103813852
GFR: 102038147
FAB: 101821194
PHI: 102104333
PMAJ: 107206790
CCAE: 111931270
CCW: 104695563
ETL: 114071768
FPG: 101910363
FCH: 102054501
CLV: 102093575
EGZ: 104125602
NNI: 104022305
ACUN: 113481735
PADL: 103916301
AAM: 106494668
ASN: 102372221
AMJ: 102567971(CYP17A1)
PSS: 102457785
CMY: 102945773
CPIC: 101933089
PVT: 110077624
PBI: 103063631
PMUR: 107290855
TSR: 106541312
PMUA: 114597528
GJA: 107117909
XLA: 100036774(cyp17a1.L)
XTR: 100145650(cyp17a1) 101730309
DRE: 399692(cyp17a1)
IPU: 100313515(cyp17) 108275468
TRU: 100125766(cyp17a2) 100125798(cyp17a1) 115249663
ONL: 100702216(cyp17a2) 100703469(cyp17)
OLA: 100125816(cyp17) 101170547(cyp17a2)
RTP: 109937010
TNL: 113500882
FCD: 110861649
LANG: 109350024
FVE: 101291823
PMUM: 103338184
CSV: 101203222
RCU: 8266041
MESC: 110609490
VVI: 109124178
SIND: 110011731
DOSA: Os01t0227700-01(Os01g0227700) Os01t0227800-01(Os01g0227800) Os01t0544200-01(Os01g0544200) Os05t0515200-01(Os05g0515200) Os06t0267400-00(Os06g0267400) Os07t0218700-01(Os07g0218700) Os10t0514700-00(Os10g0514700) Os10t0515900-01(Os10g0515900) Os12t0640200-00(Os12g0640200)
ATS: 109732954(LOC109732954) 109733316(LOC109733316) 109734069(LOC109734069) 109735932(LOC109735932) 109737135(LOC109737135) 109737518(LOC109737518) 109738679(LOC109738679) 109741114(LOC109741114) 109742910(LOC109742910) 109743416(LOC109743416) 109744001(LOC109744001) 109751006(LOC109751006) 109752396(LOC109752396) 109755199(LOC109755199) 109759571(LOC109759571) 109767764(LOC109767764) 109769810(LOC109769810) 109772273(LOC109772273) 109773520(LOC109773520) 109773761(LOC109773761) 109773763(LOC109773763) 109773846(LOC109773846) 109774510(LOC109774510) 109775751(LOC109775751) 109778245(LOC109778245) 109780102(LOC109780102) 109780749(LOC109780749) 109781183(LOC109781183) 109783097(LOC109783097) 109783333(LOC109783333) 109784156(LOC109784156) 109785019(LOC109785019)
DFA: DFA_06319(CYP514A2) DFA_07667(CYP508D1)
 » show all
Taxonomy
Reference
1  [PMID:13549484]
  Authors
LYNN WS Jr, BROWN RH.
  Title
The conversion of progesterone to androgens by testes.
  Journal
J Biol Chem 232:1015-30 (1958)
Reference
2  [PMID:6966286]
  Authors
Yoshida KI, Oshima H, Troen P.
  Title
Studies of the human testis. XIII. Properties of nicotinamide adenine dinucleotide (reduced form)-linked 17 alpha-hydroxylation.
  Journal
J Clin Endocrinol Metab 50:895-9 (1980)
DOI:10.1210/jcem-50-5-895
Reference
3  [PMID:12693981]
  Authors
Gilep AA, Estabrook RW, Usanov SA
  Title
Molecular cloning and heterologous expression in E. coli of cytochrome P45017alpha. Comparison of structural and functional properties of substrate-specific cytochromes P450 from different species.
  Journal
Biochemistry (Mosc) 68:86-98 (2003)
  Sequence
Reference
4  [PMID:17386955]
  Authors
Kolar NW, Swart AC, Mason JI, Swart P
  Title
Functional expression and characterisation of human cytochrome P45017alpha in Pichia pastoris.
  Journal
J Biotechnol 129:635-44 (2007)
DOI:10.1016/j.jbiotec.2007.02.003
Reference
5  [PMID:18707589]
  Authors
Pechurskaya TA, Lukashevich OP, Gilep AA, Usanov SA
  Title
Engineering, expression, and purification of "soluble" human cytochrome P45017alpha and its functional characterization.
  Journal
Biochemistry (Mosc) 73:806-11 (2008)
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.19
IUBMB Enzyme Nomenclature: 1.14.14.19
ExPASy - ENZYME nomenclature database: 1.14.14.19
BRENDA, the Enzyme Database: 1.14.14.19
CAS: 9029-67-8

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