Entry
Name
taxoid 1beta-hydroxylase;
taxane 1beta-hydroxylase;
CYP725A23-1;
T1betaOH
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
10-deacetyl-1-deoxybaccatin III,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (1beta-hydroxylating)
Reaction(IUBMB)
10-deacetyl-1-deoxybaccatin III + [reduced NADPH---hemoprotein reductase] + O2 = 10-deacetylbaccatin III + [oxidized NADPH---hemoprotein reductase] + H2O [RN:
R13494 ]
Reaction(KEGG)
Substrate
10-deacetyl-1-deoxybaccatin III [CPD:
C23034 ];
[reduced NADPH---hemoprotein reductase] [CPD:
C03024 ];
O2 [CPD:
C00007 ]
Product
10-deacetylbaccatin III [CPD:
C11700 ];
[oxidized NADPH---hemoprotein reductase] [CPD:
C03161 ];
H2O [CPD:
C00001 ]
Comment
A cytochrome-P-450 (heme thiolate) enzyme involved in paclitaxel (taxol) biosynthesis. Isolated from the plant Taxus baccata (English yew).
History
EC 1.14.14.192 created 2025
Reference
Authors
Zhang Y, Wiese L, Fang H, Alseekh S, Perez de Souza L, Scossa F, Molloy J, Christmann M, Fernie AR.
Title
Synthetic biology identifies the minimal gene set required for paclitaxel biosynthesis in a plant chassis.
Journal
Other DBs