KEGG   ENZYME: 1.14.14.21
Entry
EC 1.14.14.21               Enzyme                                 
Name
dibenzothiophene monooxygenase;
dszC (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
dibenzothiophene,FMNH2:oxygen oxidoreductase
Reaction(IUBMB)
dibenzothiophene + 2 FMNH2 + 2 O2 = dibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O (overall reaction) [RN:R11163];
(1a) dibenzothiophene + FMNH2 + O2 = dibenzothiophene-5-oxide + FMN + H2O [RN:R11161];
(1b) dibenzothiophene-5-oxide + FMNH2 + O2 = dibenzothiophene-5,5-dioxide + FMN + H2O [RN:R11162]
Reaction(KEGG)
Substrate
dibenzothiophene [CPD:C20125];
FMNH2 [CPD:C01847];
O2 [CPD:C00007];
dibenzothiophene-5-oxide [CPD:C21149]
Product
dibenzothiophene-5,5-dioxide [CPD:C21150];
FMN [CPD:C00061];
H2O [CPD:C00001];
dibenzothiophene-5-oxide [CPD:C21149]
Comment
This bacterial enzyme catalyses the first two steps in the desulfurization pathway of dibenzothiophenes, the oxidation of dibenzothiophene into dibenzothiophene sulfone via dibenzothiophene-5-oxide. The enzyme forms a two-component system with a dedicated NADH-dependent FMN reductase (EC 1.5.1.42) encoded by the dszD gene, which also interacts with EC 1.14.14.22, dibenzothiophene sulfone monooxygenase.
History
EC 1.14.14.21 created 2016
Orthology
K22219  dibenzothiophene monooxygenase
Genes
MAVMAV_3806
MYOOEM_10440 OEM_p100040
MGOAFA91_16295
MBRDMBRA_09620
MHERK3U94_11710
MBOKMBOE_20020
MFGK6L26_01620 K6L26_21990
MABLMMASJCM_2559
GORKTR9_5375
GTABCM27_00620
GHNMVF96_00600
 » show all
Reference
1  [PMID:9634856]
  Authors
Gray KA, Pogrebinsky OS, Mrachko GT, Xi L, Monticello DJ, Squires CH
  Title
Molecular mechanisms of biocatalytic desulfurization of fossil fuels.
  Journal
Nat Biotechnol 14:1705-9 (1996)
DOI:10.1038/nbt1296-1705
Reference
2  [PMID:24470304]
  Authors
Liu S, Zhang C, Su T, Wei T, Zhu D, Wang K, Huang Y, Dong Y, Yin K, Xu S, Xu P, Gu L
  Title
Crystal structure of DszC from Rhodococcus sp. XP at 1.79 A.
  Journal
Proteins 82:1708-20 (2014)
DOI:10.1002/prot.24525
Reference
3  [PMID:25627402]
  Authors
Guan LJ, Lee WC, Wang S, Ohshiro T, Izumi Y, Ohtsuka J, Tanokura M
  Title
Crystal structures of apo-DszC and FMN-bound DszC from Rhodococcus erythropolis D-1.
  Journal
FEBS J 282:3126-35 (2015)
DOI:10.1111/febs.13216
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.21
IUBMB Enzyme Nomenclature: 1.14.14.21
ExPASy - ENZYME nomenclature database: 1.14.14.21
BRENDA, the Enzyme Database: 1.14.14.21

DBGET integrated database retrieval system