Entry |
|
Name |
cholesterol 7alpha-monooxygenase;
cholesterol 7alpha-hydroxylase;
CYP7A1 (gene name)
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Class |
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
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Sysname |
cholesterol,NADPH---hemoprotein reductase:oxygen oxidoreductase (7alpha-hydroxylating)
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Reaction(IUBMB) |
cholesterol + [reduced NADPH---hemoprotein reductase] + O2 = 7alpha-hydroxycholesterol + [oxidized NADPH---hemoprotein reductase] + H2O [RN: R01463]
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Reaction(KEGG) |
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Substrate |
cholesterol [CPD: C00187];
[reduced NADPH---hemoprotein reductase] [CPD: C03024];
O2 [CPD: C00007]
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Product |
7alpha-hydroxycholesterol [CPD: C03594];
[oxidized NADPH---hemoprotein reductase] [CPD: C03161];
H2O [CPD: C00001]
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Comment |
A P-450 heme-thiolate liver protein that catalyses the first step in the biosynthesis of bile acids. The direct electron donor to the enzyme is EC 1.6.2.4, NADPH---hemoprotein reductase.
|
History |
EC 1.14.14.23 created 1976 as EC 1.14.13.17, transferred 2016 to EC 1.14.14.23
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Pathway |
ec00120 | Primary bile acid biosynthesis |
ec00140 | Steroid hormone biosynthesis |
|
Orthology |
K00489 | cholesterol 7alpha-monooxygenase |
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Genes |
» show all
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Reference |
|
Authors |
Mitton JR, Scholan NA, Boyd GS. |
Title |
The oxidation of cholesterol in rat liver sub-cellular particles. The cholesterol-7-alpha-Hydroxylase enzyme system. |
Journal |
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Reference |
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Authors |
Boyd GS, Grimwade AM, Lawson ME. |
Title |
Studies on rat-liver microsomal cholesterol 7alpha-hydroxylase. |
Journal |
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Reference |
|
Authors |
Ogishima T, Deguchi S, Okuda K. |
Title |
Purification and characterization of cholesterol 7 alpha-hydroxylase from rat liver microsomes. |
Journal |
J Biol Chem 262:7646-50 (1987) |
Reference |
|
Authors |
Nguyen LB, Shefer S, Salen G, Ness G, Tanaka RD, Packin V, Thomas P, Shore V, Batta A |
Title |
Purification of cholesterol 7 alpha-hydroxylase from human and rat liver and production of inhibiting polyclonal antibodies. |
Journal |
J Biol Chem 265:4541-6 (1990) |
Reference |
|
Authors |
Nguyen LB, Shefer S, Salen G, Chiang JY, Patel M |
Title |
Cholesterol 7alpha-hydroxylase activities from human and rat liver are modulated in vitro posttranslationally by phosphorylation/dephosphorylation. |
Journal |
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Other DBs |
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