KEGG   ENZYME: 1.14.14.24
Entry
EC 1.14.14.24               Enzyme                                 
Name
vitamin D 25-hydroxylase;
vitamin D2 25-hydroxylase;
vitamin D3 25-hydroxylase;
CYP2R1
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
calciol,NADPH---hemoprotein reductase:oxygen oxidoreductase (25-hydroxylating)
Reaction(IUBMB)
calciol + O2 + [reduced NADPH---hemoprotein reductase] = calcidiol + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R03611]
Reaction(KEGG)
R03611
Substrate
calciol [CPD:C05443];
O2 [CPD:C00007];
[reduced NADPH---hemoprotein reductase] [CPD:C03024]
Product
calcidiol [CPD:C01561];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001]
Comment
A microsomal enzyme isolated from human and mouse liver that bioactivates vitamin D3. While multiple isoforms (CYP27A1, CYP2J2/3, CYP3A4, CYP2D25 and CYP2C11) are able to catalyse the reaction in vitro, only CYP2R1 is thought to catalyse the reaction in humans in vivo [4]. The direct electron donor to the enzyme is EC 1.6.2.4, NADPH---hemoprotein reductase.
History
EC 1.14.14.24 created 2012 as EC 1.14.13.159, transferred 2016 to EC 1.14.14.24
Pathway
ec00100  Steroid biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K07419  vitamin D 25-hydroxylase
Genes
HSA120227(CYP2R1)
PTR451042(CYP2R1)
PPS100973299(CYP2R1)
GGO101150033
PON100460063
NLE100593944
MCC698834(CYP2R1)
MCF102138775(CYP2R1)
CSAB103239784(CYP2R1)
CATY105595724
PANU101019230
TGE112606070
RRO104681937
RBB108535517
TFN117080292
PTEH111555069
CJC100394527(CYP2R1)
SBQ101037293
CSYR103270405
MMUR105879993
LCAT123642477
OGA100961883
MMU244209(Cyp2r1)
MCAL110298348
MPAH110332047
RNO361631(Cyp2r1)
MCOC116102828
MUN110549426
CGE100772751
MAUA101831768
PLEU114697605
MORG121457797
AAMP119823353
NGI103750427
HGL101724787
CPOC100714928
CCAN109695364
DORD105999202
DSP122117854
NCAR124958302
OCU100353077
OPI101521015
TUP102495100
CFA612993(CYP2R1)
CLUD112667671
VVP112927044
VLG121476561
AML100483022
UMR103682376
UAH113246269
UAR123791666
ELK111159482
LLV125079613
MPUF101694301
ORO101369394
EJU114219310
ZCA113914132
MLX118004151
FCA101093889
PYU121018173
PBG122483590
PTG102966741
PPAD109271176
AJU106989115
HHV120244981
BTA541302(CYP2R1)
BOM102285419
BIU109569609
BBUB102394445
CHX102190621
OAS101117786
ODA120876423
CCAD122450097
SSC100124375(CYP2R1)
CFR102514910
CBAI105076609
CDK105086686
VPC102538766
BACU103007249
LVE103091120
OOR101270072
DLE111165268
PCAD102978739
PSIU116758785
ECB100146381(CYP2R1)
EPZ103557934
EAI106830430
MYB102258907
MYD102762858
MMYO118664863
MLF102430832
MNA107523752
PKL118712446
HAI109388260
DRO112303455
SHON118989725
AJM119053117
PDIC114499905
PHAS123810105
MMF118628167
RFQ117029588
PALE102894539
PGIG120603242
PVP105301484
RAY107519432
MJV108387781
TOD119255496
SARA101554146
LAV100673483
TMU101356008
DNM101439538
MDO100029137
GAS123251568
SHR100934288
PCW110220995
OAA100088947
GGA423065(CYP2R1)
PCOC116242990
MGP100547524 104910999
CJO107314632
NMEL110401886
APLA101801210
ACYG106033854
AFUL116489859
TGU100229624
LSR110468080
SCAN103827030
PMOA120501096
OTC121341489
PRUF121360922
GFR102036046
FAB101814027
PHI102099647
PMAJ107205333
CCW104683405
CBRC103625457
ETL114060398
ZAB102073058
FPG101919940
FCH102048327
CLV102097856
EGZ104123157
NNI104010937
PLET104620107
PCRI104030893
ACUN113481193
TALA104356256
PADL103923809
ACHC115352090
HALD104320456
CCRI104159955
CSTI104560569
EHS104504787
CMAC104479450
FGA104083213
GSTE104257651
LDI104342735
MNB103781454
OHA104330140
AAM106497145
AROW112968449
NPD112948840
DNE112985674
ASN102371536
AMJ102564928(CYP2R1)
CPOO109321085
GGN109299111
PSS102462921
CMY102946167
CPIC101933629
TST117877234
CABI116829045
MRV120403720
ACS100556624
PVT110083178
SUND121918991
PBI103054995
PMUR107295021 107295024
TSR106547328
PGUT117666157
VKO123021385
PMUA114598543
ZVI118087326
GJA107120114
STOW125425779
XLA108713716(cyp2r1.L) 108715308(cyp2r1.S)
XTR100127674(cyp2r1)
NPR108802409
RTEM120916935
BBUF120980988
BGAR122920379
DRE563369(cyp2r1)
SRX107750548
SANH107688167 107700616
SGH107585944 107594282
CCAR109094296
CAUA113049462 113052025
PPRM120490959
MAMB125264640
IPU108271345
PHYP113536444
SMEO124391715
TFD113662954
AMEX103038630
EEE113575769
TRU101071742
TNGGSTEN00017808G001
LCO104919371
NCC104965174
CGOB115004751
ELY117257992
EFO125901428
PLEP121944915
SLUC116067667
ECRA117951119
PFLV114557871
GAT120807653
PPUG119226474
MSAM119890509
CUD121510343
ALAT119017554
MZE101470180
ONL100695816
OAU116327197
OLA101164878
OML112160760
XMA102237962
XCO114143618
XHE116719072
PRET103462441
PFOR103139442
PLAI106960642
PMEI106921706
GAF122821740
CVG107085571
CTUL119782678
GMU124867186
NFU107381435
KMR108250436
ALIM106530854
NWH119427863
AOCE111563276
MCEP125005498
CSEM103379286
POV109636741
SSEN122772047
HHIP117755397
HSP118107013
LCF108888818
SDU111236567
SLAL111654496
XGL120795484
HCQ109525712
BPEC110172818
MALB109963208
BSPL114851861
SASA106562333 106587531
OTW112235131 112263818
OMY110506679 110526546
OGO124033625 124035615
ONE115116668 115121138
SALP111969491 111975524
SNH120025336 120056899
CCLU121556199 121582909
ELS105022275
SFM108934550 108940274
PKI111842773
AANG118227173
LOC102694258
PSPA121294963
ARUT117432425
LCM102347406
CMK103175087
RTP109926988 109927197
SCLV120335125
BCOO119085253
PPOI119106173
DSV119459200
RSAN119406493 119406501
SDM118179738
HRF124143094
MMER123525246 123537876 123537877
 » show all
Reference
1  [PMID:12867411]
  Authors
Cheng JB, Motola DL, Mangelsdorf DJ, Russell DW
  Title
De-orphanization of cytochrome P450 2R1: a microsomal vitamin D 25-hydroxilase.
  Journal
J Biol Chem 278:38084-93 (2003)
DOI:10.1074/jbc.M307028200
  Sequence
[hsa:120227] [mmu:244209]
Reference
2  [PMID:15465040]
  Authors
Shinkyo R, Sakaki T, Kamakura M, Ohta M, Inouye K
  Title
Metabolism of vitamin D by human microsomal CYP2R1.
  Journal
Biochem Biophys Res Commun 324:451-7 (2004)
DOI:10.1016/j.bbrc.2004.09.073
  Sequence
[hsa:120227]
Reference
3  [PMID:18511070]
  Authors
Strushkevich N, Usanov SA, Plotnikov AN, Jones G, Park HW
  Title
Structural analysis of CYP2R1 in complex with vitamin D3.
  Journal
J Mol Biol 380:95-106 (2008)
DOI:10.1016/j.jmb.2008.03.065
  Sequence
[hsa:120227]
Reference
4  [PMID:22310641]
  Authors
Zhu J, Deluca HF
  Title
Vitamin D 25-hydroxylase - Four decades of searching, are we there yet?
  Journal
Arch Biochem Biophys 523:30-6 (2012)
DOI:10.1016/j.abb.2012.01.013
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.24
IUBMB Enzyme Nomenclature: 1.14.14.24
ExPASy - ENZYME nomenclature database: 1.14.14.24
BRENDA, the Enzyme Database: 1.14.14.24

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