KEGG   ENZYME: 1.14.14.24
Entry
EC 1.14.14.24               Enzyme                                 

Name
vitamin D 25-hydroxylase;
vitamin D2 25-hydroxylase;
vitamin D3 25-hydroxylase;
CYP2R1
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
calciol,NADPH---hemoprotein reductase:oxygen oxidoreductase (25-hydroxylating)
Reaction(IUBMB)
calciol + O2 + [reduced NADPH---hemoprotein reductase] = calcidiol + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R03611]
Reaction(KEGG)
R03611
Substrate
calciol [CPD:C05443];
O2 [CPD:C00007];
[reduced NADPH---hemoprotein reductase] [CPD:C03024]
Product
calcidiol [CPD:C01561];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001]
Comment
A microsomal enzyme isolated from human and mouse liver that bioactivates vitamin D3. While multiple isoforms (CYP27A1, CYP2J2/3, CYP3A4, CYP2D25 and CYP2C11) are able to catalyse the reaction in vitro, only CYP2R1 is thought to catalyse the reaction in humans in vivo [4]. The direct electron donor to the enzyme is EC 1.6.2.4, NADPH---hemoprotein reductase.
History
EC 1.14.14.24 created 2012 as EC 1.14.13.159, transferred 2016 to EC 1.14.14.24
Pathway
ec00100  Steroid biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K07419  vitamin D 25-hydroxylase
Genes
HSA: 120227(CYP2R1)
PTR: 451042(CYP2R1)
PPS: 100973299
GGO: 101150033
PON: 100460063
NLE: 100593944
MCC: 698834(CYP2R1)
MCF: 102138775
CSAB: 103239784(CYP2R1)
CATY: 105595724
PANU: 101019230
RRO: 104681937
RBB: 108535517
TFN: 117080292
PTEH: 111555069
CJC: 100394527(CYP2R1)
SBQ: 101037293
MMUR: 105879993
MMU: 244209(Cyp2r1)
MCAL: 110298348
MPAH: 110332047
RNO: 361631(Cyp2r1)
MCOC: 116102828
MUN: 110549426
CGE: 100772751
PLEU: 114697605
NGI: 103750427
HGL: 101724787
CCAN: 109695364
OCU: 100353077
TUP: 102495100
CFA: 612993(CYP2R1)
VVP: 112927044
VLG: 121476561
AML: 100483022
UMR: 103682376
UAH: 113246269
ORO: 101369394
ELK: 111159482
MPUF: 101694301
EJU: 114219310
MLX: 118004151
FCA: 101093889
PYU: 121018173
PTG: 102966741
PPAD: 109271176
AJU: 106989115
HHV: 120244981
BTA: 541302(CYP2R1)
BOM: 102285419
BIU: 109569609
BBUB: 102394445
CHX: 102190621
OAS: 101117786
ODA: 120876423
CCAD: 122450097
SSC: 100124375(CYP2R1)
CFR: 102514910
CBAI: 105076609
CDK: 105086686
BACU: 103007249
LVE: 103091120
OOR: 101270072
DLE: 111165268
PCAD: 102978739
ECB: 100146381(CYP2R1)
EPZ: 103557934
EAI: 106830430
MYB: 102258907
MYD: 102762858
MMYO: 118664863
MNA: 107523752
HAI: 109388260
DRO: 112303455
SHON: 118989725
AJM: 119053117
MMF: 118628167
PALE: 102894539
PGIG: 120603242
RAY: 107519432
MJV: 108387781
TOD: 119255496
LAV: 100673483
TMU: 101356008
MDO: 100029137
SHR: 100934288
PCW: 110220995
OAA: 100088947
GGA: 423065(CYP2R1)
PCOC: 116242990
CJO: 107314632
NMEL: 110401886
APLA: 101801210
ACYG: 106033854
TGU: 100229624
LSR: 110468080
SCAN: 103827030
PMOA: 120501096
GFR: 102036046
FAB: 101814027
PHI: 102099647
PMAJ: 107205333
CCW: 104683405
ETL: 114060398
FPG: 101919940
FCH: 102048327
CLV: 102097856
EGZ: 104123157
NNI: 104010937
ACUN: 113481193
PADL: 103923809
AAM: 106497145
NPD: 112948840
ASN: 102371536
AMJ: 102564928(CYP2R1)
CPOO: 109321085
GGN: 109299111
PSS: 102462921
CMY: 102946167
CPIC: 101933629
TST: 117877234
CABI: 116829045
ACS: 100556624
PVT: 110083178
PBI: 103054995
TSR: 106547328
PGUT: 117666157
PMUA: 114598543
ZVI: 118087326
GJA: 107120114
XLA: 108713716(cyp2r1.L) 108715308(cyp2r1.S)
XTR: 100127674(cyp2r1)
NPR: 108802409
DRE: 563369(cyp2r1)
SRX: 107750548
CCAR: 109094296
IPU: 108271345(Cyp2r1)
PHYP: 113536444
AMEX: 103038630
EEE: 113575769
TRU: 101071742
LCO: 104919371
NCC: 104965174
CGOB: 115004751
ELY: 117257992
PLEP: 121944915
SLUC: 116067667
ECRA: 117951119
PFLV: 114557871
GAT: 120807653
MSAM: 119890509
CUD: 121510343
MZE: 101470180
ONL: 100695816
OAU: 116327197
OLA: 101164878
OML: 112160760
XMA: 102237962
XCO: 114143618
XHE: 116719072
PRET: 103462441
CVG: 107085571
CTUL: 119782678
NFU: 107381435
KMR: 108250436
ALIM: 106530854
AOCE: 111563276
CSEM: 103379286
POV: 109636741
LCF: 108888818
SDU: 111236567
SLAL: 111654496
XGL: 120795484
HCQ: 109525712
BPEC: 110172818
MALB: 109963208
ELS: 105022275
PKI: 111842773
AANG: 118227173
LOC: 102694258
PSPA: 121294963
ARUT: 117432425
LCM: 102347406
CMK: 103175087
SCLV: 120335125
DSV: 119459200
SDM: 118179738
 » show all
Reference
1  [PMID:12867411]
  Authors
Cheng JB, Motola DL, Mangelsdorf DJ, Russell DW
  Title
De-orphanization of cytochrome P450 2R1: a microsomal vitamin D 25-hydroxilase.
  Journal
J Biol Chem 278:38084-93 (2003)
DOI:10.1074/jbc.M307028200
  Sequence
[hsa:120227] [mmu:244209]
Reference
2  [PMID:15465040]
  Authors
Shinkyo R, Sakaki T, Kamakura M, Ohta M, Inouye K
  Title
Metabolism of vitamin D by human microsomal CYP2R1.
  Journal
Biochem Biophys Res Commun 324:451-7 (2004)
DOI:10.1016/j.bbrc.2004.09.073
  Sequence
[hsa:120227]
Reference
3  [PMID:18511070]
  Authors
Strushkevich N, Usanov SA, Plotnikov AN, Jones G, Park HW
  Title
Structural analysis of CYP2R1 in complex with vitamin D3.
  Journal
J Mol Biol 380:95-106 (2008)
DOI:10.1016/j.jmb.2008.03.065
  Sequence
[hsa:120227]
Reference
4  [PMID:22310641]
  Authors
Zhu J, Deluca HF
  Title
Vitamin D 25-hydroxylase - Four decades of searching, are we there yet?
  Journal
Arch Biochem Biophys 523:30-6 (2012)
DOI:10.1016/j.abb.2012.01.013
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.24
IUBMB Enzyme Nomenclature: 1.14.14.24
ExPASy - ENZYME nomenclature database: 1.14.14.24
BRENDA, the Enzyme Database: 1.14.14.24

DBGET integrated database retrieval system