KEGG   ENZYME: 1.14.14.27Help
Entry
EC 1.14.14.27               Enzyme                                 

Name
resorcinol 4-hydroxylase (FADH2);
graA (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
resorcinol,FADH2:oxygen oxidoreductase (4-hydroxylating)
Reaction(IUBMB)
resorcinol + FADH2 + O2 = hydroxyquinol + FAD + H2O [RN:R11348]
Reaction(KEGG)
Substrate
resorcinol [CPD:C01751];
FADH2 [CPD:C01352];
O2 [CPD:C00007]
Product
hydroxyquinol [CPD:C02814];
FAD [CPD:C00016];
H2O [CPD:C00001]
Comment
The enzyme, characterized from the bacterium Rhizobium sp. strain MTP-10005, uses FADH2 as a substrate rather than a cofactor. FADH2 is provided by a dedicated EC 1.5.1.36, flavin reductase (NADH). The enzyme participates in the degradation of gamma-resorcylate and resorcinol. cf. EC 1.14.13.220, resorcinol 4-hydroxylase (NADH), and EC 1.14.13.219, resorcinol 4-hydroxylase (NADPH).
History
EC 1.14.14.27 created 2016
Pathway
ec00362  Benzoate degradation
ec01120  Microbial metabolism in diverse environments
Orthology
K20942  resorcinol 4-hydroxylase (FADH2)
Genes
CNC: CNE_BB1p12850(hsaA)
CPAU: EHF44_23910
BSEM: WJ12_23750
BUG: BC1001_0584
BGO: BM43_4094
BPX: BUPH_04474
PHS: C2L64_23505
PPNO: DA70_09470
PPNM: LV28_21340
PRB: X636_17090
POL: Bpro_2060
AAA: Acav_4145
SCL: sce4164
ATU: Atu2530
ARA: Arad_7161
RLE: pRL120217
SWI: Swit_3090
 » show all
Taxonomy
Reference
1  [PMID:1280]
  Authors
Ohta Y, Ribbons DW
  Title
Bacterial metabolism of resorcinylic compounds: purification and properties of orcinol hydroxylase and resorcinol hydroxylase from Pseudomonas putida ORC.
  Journal
Eur J Biochem 61:259-69 (1976)
DOI:10.1111/j.1432-1033.1976.tb10019.x
Reference
2  [PMID:17158677]
  Authors
Yoshida M, Oikawa T, Obata H, Abe K, Mihara H, Esaki N
  Title
Biochemical and genetic analysis of the gamma-resorcylate (2,6-dihydroxybenzoate) catabolic pathway in Rhizobium sp. strain MTP-10005: identification and functional analysis of its gene cluster.
  Journal
J Bacteriol 189:1573-81 (2007)
DOI:10.1128/JB.01675-06
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.27
IUBMB Enzyme Nomenclature: 1.14.14.27
ExPASy - ENZYME nomenclature database: 1.14.14.27
BRENDA, the Enzyme Database: 1.14.14.27

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