KEGG   ENZYME: 1.14.14.27
Entry
EC 1.14.14.27               Enzyme                                 
Name
resorcinol 4-hydroxylase (FADH2);
graA (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
resorcinol,FADH2:oxygen oxidoreductase (4-hydroxylating)
Reaction(IUBMB)
resorcinol + FADH2 + O2 = hydroxyquinol + FAD + H2O [RN:R11348]
Reaction(KEGG)
R11348
Substrate
resorcinol [CPD:C01751];
FADH2 [CPD:C01352];
O2 [CPD:C00007]
Product
hydroxyquinol [CPD:C02814];
FAD [CPD:C00016];
H2O [CPD:C00001]
Comment
The enzyme, characterized from the bacterium Rhizobium sp. strain MTP-10005, uses FADH2 as a substrate rather than a cofactor. FADH2 is provided by a dedicated EC 1.5.1.36, flavin reductase (NADH). The enzyme participates in the degradation of gamma-resorcylate and resorcinol. cf. EC 1.14.13.220, resorcinol 4-hydroxylase (NADH), and EC 1.14.13.219, resorcinol 4-hydroxylase (NADPH).
History
EC 1.14.14.27 created 2016
Pathway
ec00362  Benzoate degradation
ec01120  Microbial metabolism in diverse environments
Orthology
K20942  resorcinol 4-hydroxylase (FADH2)
Genes
PPAVLOZ86_13655
CNCCNE_BB1p12850(hsaA)
CPAUEHF44_23910
BSEMWJ12_23750
BUGBC1001_0584
BGOBM43_4094
BPXBUPH_04474
PHSC2L64_23505
PPNODA70_09470
PPNMLV28_21340
PRBX636_17090
POXMB84_08075
PUDG9Q38_01850
POLBpro_2060
AAAAcav_4145
VAPVapar_5306
SCLsce4164
SCUSCE1572_16175
RPODE0E05_16190
ESJSJ05684_b42840
ATUAtu2530
ARAArad_7161
AGRAGROH133_08585
ATFAch5_23990
AROB0909_00290
REPIE4803_PC00117
REIIE4771_PD00117
RLEpRL120217
RLTRleg2_5725
RLBRLEG3_09700
RLURLEG12_02375
RHKKim5_PC00120
RADCO657_29285
ROYG3A56_13310
RIIFFM53_025670
RHIDFFM81_029545
RBQJ2J99_28405
RRGJ3P73_26390
BICLMTR13_14525
RHZRHPLAN_21220 RHPLAN_61360 RHPLAN_65290
PSINCAK95_00050 CAK95_00315
SWISwit_3090
SPHDHY78_10125 HY78_22020
SYBTZ53_18205
SBDATN00_17695
SHYDCJD35_15750
SUFLFIL70_06575
RGIRGI145_20905
REBXU06_29875
SCBSCAB_14031
YIALO772_34270
MCAWF6J84_06565
FRPAX769_00875
ARZAUT26_02440
APRECNX65_23440
AMIAmir_4707
 » show all
Reference
1  [PMID:1280]
  Authors
Ohta Y, Ribbons DW
  Title
Bacterial metabolism of resorcinylic compounds: purification and properties of orcinol hydroxylase and resorcinol hydroxylase from Pseudomonas putida ORC.
  Journal
Eur J Biochem 61:259-69 (1976)
DOI:10.1111/j.1432-1033.1976.tb10019.x
Reference
2  [PMID:17158677]
  Authors
Yoshida M, Oikawa T, Obata H, Abe K, Mihara H, Esaki N
  Title
Biochemical and genetic analysis of the gamma-resorcylate (2,6-dihydroxybenzoate) catabolic pathway in Rhizobium sp. strain MTP-10005: identification and functional analysis of its gene cluster.
  Journal
J Bacteriol 189:1573-81 (2007)
DOI:10.1128/JB.01675-06
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.27
IUBMB Enzyme Nomenclature: 1.14.14.27
ExPASy - ENZYME nomenclature database: 1.14.14.27
BRENDA, the Enzyme Database: 1.14.14.27

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