KEGG   ENZYME: 1.14.14.28Help
Entry
EC 1.14.14.28               Enzyme                                 

Name
long-chain alkane monooxygenase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
long-chain-alkane,FMNH2:oxygen oxidoreductase
Reaction(IUBMB)
a long-chain alkane + FMNH2 + O2 = a long-chain primary alcohol + FMN + H2O
Substrate
long-chain alkane [CPD:C21763];
FMNH2 [CPD:C01847];
O2 [CPD:C00007]
Product
long-chain primary alcohol;
FMN [CPD:C00061];
H2O [CPD:C00001]
Comment
The enzyme, characterized from the bacterium Geobacillus thermodenitrificans NG80-2, is capable of converting alkanes ranging from C15 to C36 into their corresponding primary alcohols [1,2]. The FMNH2 cofactor is provided by an FMN reductase [3].
History
EC 1.14.14.28 created 2016
Orthology
K20938  long-chain alkane monooxygenase
Genes
KPM: KPHS_42240
KPP: A79E_0950
KPH: KPNIH24_07680
KPZ: KPNIH27_20450
KPV: KPNIH29_21210
KPW: KPNIH30_21230
KPY: KPNIH31_20495
KPG: KPNIH32_22090
KPC: KPNIH10_21030
KPQ: KPR0928_20515
KPR: KPR_4159
KPJ: N559_1083
KPX: PMK1_00628(dmoA)
KPNU: LI86_05350
KPNK: BN49_0967
KVA: Kvar_0908
KIE: NCTC12125_01689(snaA) NCTC12125_01690(ntaA_1)
EBF: D782_2584
SRL: SOD_c23360(dmoA)
SPLY: Q5A_012910(dmoA)
SERF: L085_12615
SOD: Sant_2221
PLF: PANA5342_1855(soxA)
PAJ: PAJ_1557(soxA)
PSTW: DSJ_15500
PCQ: PcP3B5_03710(dmoA_1) PcP3B5_25100(dmoA_2)
PPU: PP_0222 PP_2746(dmoA-I)
PSB: Psyr_0352
PFL: PFL_0243
PPRC: PFLCHA0_c02440(soxA1) PFLCHA0_c26840(soxA3)
PFB: VO64_3293
PMAN: OU5_3247
PEN: PSEEN0201
PSES: PSCI_0883
PSEC: CCOS191_0195(dmoA)
PSIL: PMA3_00690
AVN: Avin_43810(seuA)
ABY: ABAYE2215
ABN: AB57_1671
ABX: ABK1_1930
ABH: M3Q_1832
ABAD: ABD1_14390
ABAZ: P795_10015
ABAU: IX87_03045
ABAA: IX88_09150
ACC: BDGL_000822(y4fC)
AMAH: DLM_1520
BMA: BMAA1146
BMAL: DM55_4402
BMAE: DM78_3444
BMAQ: DM76_3352
BMAI: DM57_09645
BMAF: DM51_4815
BMAZ: BM44_4568
BMAB: BM45_3693
BPS: BPSS0686
BPSE: BDL_3916
BPSM: BBQ_5508
BPSU: BBN_4086
BPSD: BBX_5753
BPK: BBK_3804
BPSH: DR55_4664
BPSA: BBU_5357
BPSO: X996_3868
BUT: X994_3931
BTQ: BTQ_5029
BTJ: BTJ_3656
BTZ: BTL_4503
BTD: BTI_5494
BTV: BTHA_5714
BTHE: BTN_4154
BTHM: BTRA_3939
BTHA: DR62_4189
BTHL: BG87_4485
BOK: DM82_4765
BOC: BG90_5828
BPSL: WS57_18425
BSTG: WT74_17450
BGU: KS03_4892
BGO: BM43_5333
BUL: BW21_5177
BXB: DR64_6850
PLG: NCTC10937_05008(moxC_2)
AXX: ERS451415_00326(ntaA_1)
AMIM: MIM_c35060
ODI: ODI_R0032
JAG: GJA_1813(ssuD)
CFU: CFU_2748
CARE: LT85_1890
RLE: pRL90300
AOL: S58_11450
BBAR: RHAL1_3382(dmoA)
GOH: B932_3153
GXY: GLX_02420
GXL: H845_112
KEU: S101446_02193(ssuD)
KSC: CD178_03010(dmoA)
ASZ: ASN_3307(ladA)
BMQ: BMQ_0870
BMEG: BG04_3163
BMET: BMMGA3_09055(dmoA)
GTN: GTNG_3499(ladA)
SDT: SPSE_0204
SSCZ: RN70_01705
PRI: PRIO_3213(dmoA1) PRIO_3222(dmoA3)
MAO: MAP4_0562
MAVI: RC58_02730
MAVU: RE97_02735
MSHG: MSG_01346
MPHL: MPHLCCUG_00582(dmoA_1) MPHLCCUG_00583(dmoA_2)
MHAS: MHAS_00043(dmoA_1) MHAS_01320(dmoA_4) MHAS_04300(dmoA_5)
MABB: MASS_2403
MCHE: BB28_12440
MSTE: MSTE_02425
MSAL: DSM43276_02206(dmoA_3)
RFA: A3L23_00433(dmoA)
RHS: A3Q41_02974(dmoA)
GOR: KTR9_4334
SMAL: SMALA_8129
KSK: KSE_14950
MMAR: MODMU_1490
AJA: AJAP_03400(dmoA)
AMQ: AMETH_2489(ssuD)
PSEE: FRP1_05610
PSEH: XF36_17750
HHB: Hhub_1296(dmoA)
 » show all
Taxonomy
Reference
1  [PMID:17372208]
  Authors
Feng L, Wang W, Cheng J, Ren Y, Zhao G, Gao C, Tang Y, Liu X, Han W, Peng X, Liu R, Wang L
  Title
Genome and proteome of long-chain alkane degrading Geobacillus thermodenitrificans NG80-2 isolated from a deep-subsurface oil reservoir.
  Journal
Proc Natl Acad Sci U S A 104:5602-7 (2007)
DOI:10.1073/pnas.0609650104
  Sequence
[gtn:GTNG_3499]
Reference
2  [PMID:18164311]
  Authors
Li L, Liu X, Yang W, Xu F, Wang W, Feng L, Bartlam M, Wang L, Rao Z
  Title
Crystal structure of long-chain alkane monooxygenase (LadA) in complex with coenzyme FMN: unveiling the long-chain alkane hydroxylase.
  Journal
J Mol Biol 376:453-65 (2008)
DOI:10.1016/j.jmb.2007.11.069
  Sequence
[gtn:GTNG_3499]
Reference
3  [PMID:22526792]
  Authors
Dong Y, Yan J, Du H, Chen M, Ma T, Feng L
  Title
Engineering of LadA for enhanced hexadecane oxidation using random- and site-directed mutagenesis.
  Journal
Appl Microbiol Biotechnol 94:1019-29 (2012)
DOI:10.1007/s00253-012-4035-y
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.28
IUBMB Enzyme Nomenclature: 1.14.14.28
ExPASy - ENZYME nomenclature database: 1.14.14.28
BRENDA, the Enzyme Database: 1.14.14.28

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