KEGG   ENZYME: 1.14.14.29Help
Entry
EC 1.14.14.29               Enzyme                                 

Name
25/26-hydroxycholesterol 7alpha-hydroxylase;
25-hydroxycholesterol 7alpha-monooxygenase;
CYP7B1;
CYP7B1 oxysterol 7alpha-hydroxylase;
27-hydroxycholesterol 7-monooxygenase;
27-hydroxycholesterol 7alpha-hydroxylase;
cholest-5-ene-3beta,25-diol,NADPH:oxygen oxidoreductase (7alpha-hydroxylating);
25-hydroxycholesterol 7alpha-hydroxylase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
cholest-5-ene-3beta,25/26-diol,[NADPH---hemoprotein reductase]:oxygen oxidoreductase (7alpha-hydroxylating)
Reaction(IUBMB)
(1) cholest-5-ene-3beta,25-diol + [reduced NADPH---hemoprotein reductase] + O2 = cholest-5-ene-3beta,7alpha,25-triol + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R07209];
(2) (25R)-cholest-5-ene-3beta,26-diol + [reduced NADPH---hemoprotein reductase] + O2 = (25R)-cholest-5-ene-3beta,7alpha,26-triol + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R07372]
Reaction(KEGG)
Substrate
cholest-5-ene-3beta,25-diol [CPD:C15519];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007];
(25R)-cholest-5-ene-3beta,26-diol
Product
cholest-5-ene-3beta,7alpha,25-triol [CPD:C15520];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001];
(25R)-cholest-5-ene-3beta,7alpha,26-triol
Comment
A P-450 (heme-thiolate) protein. Unlike EC 1.14.14.26, 24-hydroxycholesterol 7alpha-monooxygenase, which is specific for its oxysterol substrate, this enzyme can also metabolize the oxysterols 24,25-epoxycholesterol, 22-hydroxycholesterol and 24-hydroxycholesterol, but to a lesser extent [2]. The direct electron donor to the enzyme is EC 1.6.2.4, NADPH---hemoprotein reductase.
History
EC 1.14.14.29 created 2005 as EC 1.14.13.100, modified 2013 (EC 1.14.13.60 created 1999, incorporated 2013), transferred 2016 to EC 1.14.14.29
Pathway
ec00120  Primary bile acid biosynthesis
ec00140  Steroid hormone biosynthesis
Orthology
K07430  25/26-hydroxycholesterol 7alpha-hydroxylase
Genes
HSA: 9420(CYP7B1)
PTR: 743817(CYP7B1)
PPS: 100995737
GGO: 101124249
PON: 100439228
NLE: 100586163
MCC: 710188
MCF: 102132453
CSAB: 103236887
RRO: 104672629
RBB: 108528811
CJC: 100385287(CYP7B1)
SBQ: 101049402
MMU: 13123(Cyp7b1)
MCAL: 110291739
MPAH: 110320479
RNO: 25429(Cyp7b1)
CGE: 100754734
NGI: 103737731
HGL: 101721563
CCAN: 109687466
OCU: 100343291
TUP: 102468171
CFA: 486973(CYP7B1)
VVP: 112929794
AML: 100477302
UMR: 103677513
UAH: 113268059
ORO: 101371391
FCA: 101086873
PTG: 102970913
PPAD: 109250237
AJU: 106985755
BTA: 529552(CYP7B1)
BOM: 102276071
BIU: 109568550
BBUB: 102399179
CHX: 102191152
OAS: 101119648
SSC: 100738003
CFR: 102508530
CDK: 105094653
BACU: 103014598
LVE: 103087141
OOR: 101272520
DLE: 111185591
PCAD: 102973949
ECB: 100064741
EPZ: 103548391
EAI: 106834315
MYB: 102240967
MYD: 102767325
MNA: 107526445
HAI: 109394857
DRO: 112315556
PALE: 102888293
RAY: 107514800
MJV: 108406746
LAV: 100673174
TMU: 101359614
MDO: 100029487(CYP7B1)
SHR: 100920521
PCW: 110219040
OAA: 100081634
GGA: 420164(CYP7B1)
MGP: 100541042
CJO: 107310250(Cyp7b1)
NMEL: 110394104
APLA: 101791081
ACYG: 106039057
TGU: 100224987
LSR: 110484735
SCAN: 103812691
GFR: 102042075
FAB: 101813216
PHI: 102104989
PMAJ: 107200525
CCAE: 111924690
CCW: 104692847
ETL: 114062632
FPG: 101918669
FCH: 102060263
CLV: 102096682
EGZ: 104122045
NNI: 104009623
ACUN: 113477067
PADL: 103923796
AAM: 106494420
ASN: 102386823
AMJ: 102562782(CYP7B1)
PSS: 102462148
CMY: 102941409
CPIC: 101932491
ACS: 100564185
PVT: 110089029
PBI: 103059038
PMUR: 107288949
TSR: 106540924
PMUA: 114600560
GJA: 107110865
XLA: 108719274(cyp7b1.L)
XTR: 100145642(cyp7b1)
NPR: 108798116
DRE: 570455(cyp7b1)
IPU: 108270649(CYP7B1)
PHYP: 113542997
AMEX: 103031147
EEE: 113584113
TRU: 101065551
LCO: 104937751
MZE: 101467563
ONL: 100694909
OLA: 101173499
XMA: 102223762
PRET: 103460470
CVG: 107100354
NFU: 107374169
KMR: 108229396
ALIM: 106533460
AOCE: 111564486
CSEM: 103398716
POV: 109623546
LCF: 108884884
SDU: 111237347
SLAL: 111670231
HCQ: 109518823
BPEC: 110164336
MALB: 109974849
SASA: 106569045 106599438(CP7B1)
ELS: 105009441
SFM: 108937483
PKI: 111861141
LCM: 102345159
CMK: 103180767
RTP: 109934694
CIN: 100184784
LAK: 106166062
 » show all
Taxonomy
Reference
1  [PMID:8496664]
  Authors
Martin KO, Budai K, Javitt NB
  Title
Cholesterol and 27-hydroxycholesterol 7 alpha-hydroxylation: evidence for two different enzymes.
  Journal
J Lipid Res 34:581-8 (1993)
Reference
2  [PMID:7925343]
  Authors
Toll A, Wikvall K, Sudjana-Sugiaman E, Kondo KH, Bjorkhem I.
  Title
7 alpha hydroxylation of 25-hydroxycholesterol in liver microsomes. Evidence that the enzyme involved is different from cholesterol 7 alpha-hydroxylase.
  Journal
Eur J Biochem 224:309-16 (1994)
DOI:10.1111/j.1432-1033.1994.00309.x
Reference
3  [PMID:10748047]
  Authors
Li-Hawkins J, Lund EG, Bronson AD, Russell DW.
  Title
Expression cloning of an oxysterol 7alpha-hydroxylase selective for 24-hydroxycholesterol.
  Journal
J Biol Chem 275:16543-9 (2000)
DOI:10.1074/jbc.M001810200
Reference
4  [PMID:12759897]
  Authors
Ren S, Marques D, Redford K, Hylemon PB, Gil G, Vlahcevic ZR, Pandak WM.
  Title
Regulation of oxysterol 7alpha-hydroxylase (CYP7B1) in the rat.
  Journal
Metabolism 52:636-42 (2003)
DOI:10.1053/meta.2003.50106
  Sequence
[rno:25429]
Reference
5  [PMID:12543708]
  Authors
Russell DW.
  Title
The enzymes, regulation, and genetics of bile acid synthesis.
  Journal
Annu Rev Biochem 72:137-74 (2003)
DOI:10.1146/annurev.biochem.72.121801.161712
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.29
IUBMB Enzyme Nomenclature: 1.14.14.29
ExPASy - ENZYME nomenclature database: 1.14.14.29
BRENDA, the Enzyme Database: 1.14.14.29
CAS: 149316-80-3

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