KEGG   ENZYME: 1.14.14.29
Entry
EC 1.14.14.29               Enzyme                                 
Name
25/26-hydroxycholesterol 7alpha-hydroxylase;
25-hydroxycholesterol 7alpha-monooxygenase;
CYP7B1;
CYP7B1 oxysterol 7alpha-hydroxylase;
27-hydroxycholesterol 7-monooxygenase;
27-hydroxycholesterol 7alpha-hydroxylase;
cholest-5-ene-3beta,25-diol,NADPH:oxygen oxidoreductase (7alpha-hydroxylating);
25-hydroxycholesterol 7alpha-hydroxylase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
cholest-5-ene-3beta,25/26-diol,[NADPH---hemoprotein reductase]:oxygen oxidoreductase (7alpha-hydroxylating)
Reaction(IUBMB)
(1) cholest-5-ene-3beta,25-diol + [reduced NADPH---hemoprotein reductase] + O2 = cholest-5-ene-3beta,7alpha,25-triol + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R07209];
(2) (25R)-cholest-5-ene-3beta,26-diol + [reduced NADPH---hemoprotein reductase] + O2 = (25R)-cholest-5-ene-3beta,7alpha,26-triol + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R07372]
Reaction(KEGG)
R07209 R07372;
(other) R08727 R08943
Substrate
cholest-5-ene-3beta,25-diol [CPD:C15519];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007];
(25R)-cholest-5-ene-3beta,26-diol
Product
cholest-5-ene-3beta,7alpha,25-triol [CPD:C15520];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001];
(25R)-cholest-5-ene-3beta,7alpha,26-triol
Comment
A P-450 (heme-thiolate) protein. Unlike EC 1.14.14.26, 24-hydroxycholesterol 7alpha-monooxygenase, which is specific for its oxysterol substrate, this enzyme can also metabolize the oxysterols 24,25-epoxycholesterol, 22-hydroxycholesterol and 24-hydroxycholesterol, but to a lesser extent [2]. The direct electron donor to the enzyme is EC 1.6.2.4, NADPH---hemoprotein reductase.
History
EC 1.14.14.29 created 2005 as EC 1.14.13.100, modified 2013 (EC 1.14.13.60 created 1999, incorporated 2013), transferred 2016 to EC 1.14.14.29
Pathway
ec00120  Primary bile acid biosynthesis
ec00140  Steroid hormone biosynthesis
Orthology
K07430  25/26-hydroxycholesterol 7alpha-hydroxylase
Genes
HSA9420(CYP7B1)
PTR743817(CYP7B1)
PPS100995737(CYP7B1)
GGO101124249
PON100439228
NLE100586163
MCC710188
MCF102132453(CYP7B1)
CSAB103236887
CATY105585207
PANU101022119
TGE112630225
RRO104672629
RBB108528811
TFN117078926
PTEH111549281
CJC100385287(CYP7B1)
SBQ101049402
CSYR103259558
MMUR105870634
LCAT123645197
OGA100945075
MMU13123(Cyp7b1)
MCAL110291739
MPAH110320479
RNO25429(Cyp7b1)
MCOC116094973
CGE100754734
MAUA101834951
PLEU114690079
MORG121458368
AAMP119826373
NGI103737731
HGL101721563
CPOC100717521
CCAN109687466
DORD105980438
DSP122095977
NCAR124987909
OCU100343291
OPI101530122
TUP102468171
CFA486973(CYP7B1)
CLUD112678529
VVP112929794
VLG121498862
AML100477302
UMR103677513
UAH113268059
UAR123786699
ELK111155386
LLV125098786
MPUF101686726
ORO101371391
EJU114215918
ZCA113939871
MLX118014870
FCA101086873
PYU121038192
PBG122495460
PTG102970913
PPAD109250237
AJU106985755
HHV120225910
BTA529552(CYP7B1)
BOM102276071
BIU109568550
BBUB102399179
CHX102191152
OAS101119648
ODA120878621
CCAD122450803
SSC100738003
CFR102508530
CBAI105081517
CDK105094653
VPC102541974
BACU103014598
LVE103087141
OOR101272520
DLE111185591
PCAD102973949
PSIU116742659
ECB100064741
EPZ103548391
EAI106834315
MYB102240967
MYD102767325
MMYO118670889
MLF102429598
MNA107526445
PKL118718312
HAI109394857
DRO112315556
SHON118995511
AJM119040492
PDIC114514660
PHAS123833187
MMF118631875
RFQ117034014
PALE102888293
PGIG120585444
PVP105295064
RAY107514800
MJV108406746
TOD119249767
SARA101548894
LAV100673174
TMU101359614
DNM101412511
MDO100029487(CYP7B1)
GAS123238852
SHR100920521
PCW110219040
OAA100081634
GGA420164(CYP7B1)
PCOC116242231
MGP100541042
CJO107310250
NMEL110394104
APLA101791081
ACYG106039057
AFUL116486476
TGU100224987
LSR110484735
SCAN103812691
PMOA120499299
OTC121340234
PRUF121356893
GFR102042075
FAB101813216
PHI102104989
PMAJ107200525
CCAE111924690
CCW104692847
CBRC103624890
ETL114062632
ZAB102075219
FPG101918669
FCH102060263
CLV102096682
EGZ104122045
NNI104009623
PLET104626684
PCRI104038879
ACUN113477067
TALA104366826
PADL103923796
ACHC115340578
HALD104311879
CCRI104163502
CSTI104551236
EHS104504799
CMAC104479943
FGA104079926
GSTE104262962
LDI104349302
MNB103778811
OHA104332314
AAM106494420
AROW112976995
NPD112944778
DNE112986694
ASN102386823
AMJ102562782(CYP7B1)
CPOO109319078
GGN109304921
PSS102462148
CMY102941409
CPIC101932491
TST117873529
CABI116838703
MRV120399008
ACS100564185
PVT110089029
SUND121927910
PBI103059038
PMUR107288949
TSR106540924
PGUT117672844
VKO123020571
PMUA114600560
ZVI118089647
GJA107110865
STOW125439248
XLA108719274(cyp7b1.L)
XTR100145642(cyp7b1)
NPR108798116
RTEM120941049
BBUF121001040
BGAR122938626
DRE570455(cyp7b1)
SRX107721742 107749124
SANH107659553 107690180
SGH107566988 107602637
CCAR109070086 109073141
CAUA113045069 113120109 113120636
PPRM120465136
MAMB125250943
IPU108270649
PHYP113542997
SMEO124402937
TFD113637322
AMEX103031147
EEE113584113
TRU101065551
TNGGSTEN00005656G001 GSTEN00010553G001
LCO104937751
CGOB115022582
ELY117272492
EFO125902090
PLEP121937590
SLUC116056359 116056373
ECRA117953909
PFLV114565353
GAT120816669
PPUG119209380
MSAM119896733 119902807
CUD121519931
ALAT119011747
MZE101467563
ONL100694909
OAU116317826
OLA101173499
OML112144318
XMA102223762
XCO114146856 114146857
XHE116720964
PRET103460470
PFOR103131102
PLAI106938689
PMEI106910762
GAF122841696
CVG107100354
CTUL119775668
GMU124869309
NFU107374169
KMR108229396
ALIM106533460
NWH119414824
AOCE111564486
MCEP125011284
CSEM103398716
SSEN122761320
HHIP117778604
HSP118118373
LCF108884884
SDU111237347
SLAL111670231
XGL120800642
HCQ109518823
MALB109974849
BSPL114844605
SASA106569045 106599438(CP7B1)
OTW112226969 112260443
OMY110508760 110530280
OGO124009594 124041887
ONE115103117 115114379
SALP111956752 111979270
SNH120020376 120055909
CCLU121535194
ELS105009441
SFM108937483
PKI111861141
LOC102692895
PSPA121313380
ARUT117399708
LCM102345159
CMK103180767
RTP109934694
BFO118409866 118419007
BBEL109463792 109465379 109468210 109472486
 » show all
Reference
1  [PMID:8496664]
  Authors
Martin KO, Budai K, Javitt NB
  Title
Cholesterol and 27-hydroxycholesterol 7 alpha-hydroxylation: evidence for two different enzymes.
  Journal
J Lipid Res 34:581-8 (1993)
Reference
2  [PMID:7925343]
  Authors
Toll A, Wikvall K, Sudjana-Sugiaman E, Kondo KH, Bjorkhem I.
  Title
7 alpha hydroxylation of 25-hydroxycholesterol in liver microsomes. Evidence that the enzyme involved is different from cholesterol 7 alpha-hydroxylase.
  Journal
Eur J Biochem 224:309-16 (1994)
DOI:10.1111/j.1432-1033.1994.00309.x
Reference
3  [PMID:10748047]
  Authors
Li-Hawkins J, Lund EG, Bronson AD, Russell DW
  Title
Expression cloning of an oxysterol 7alpha-hydroxylase selective for 24-hydroxycholesterol.
  Journal
J Biol Chem 275:16543-9 (2000)
DOI:10.1074/jbc.M001810200
Reference
4  [PMID:12759897]
  Authors
Ren S, Marques D, Redford K, Hylemon PB, Gil G, Vlahcevic ZR, Pandak WM
  Title
Regulation of oxysterol 7alpha-hydroxylase (CYP7B1) in the rat.
  Journal
Metabolism 52:636-42 (2003)
DOI:10.1053/meta.2003.50106
  Sequence
[rno:25429]
Reference
5  [PMID:12543708]
  Authors
Russell DW
  Title
The enzymes, regulation, and genetics of bile acid synthesis.
  Journal
Annu Rev Biochem 72:137-74 (2003)
DOI:10.1146/annurev.biochem.72.121801.161712
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.29
IUBMB Enzyme Nomenclature: 1.14.14.29
ExPASy - ENZYME nomenclature database: 1.14.14.29
BRENDA, the Enzyme Database: 1.14.14.29
CAS: 149316-80-3

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