Entry
Name
bacterial luciferase;
aldehyde monooxygenase;
luciferase;
Vibrio fischeri luciferase;
alkanal,reduced-FMN:oxygen oxidoreductase (1-hydroxylating, luminescing);
alkanal,FMNH2:oxygen oxidoreductase (1-hydroxylating, luminescing);
alkanal monooxygenase (FMN);
aldehyde,FMNH2:oxygen oxidoreductase (1-hydroxylating, luminescing)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
long-chain-aldehyde,FMNH2:oxygen oxidoreductase (1-hydroxylating, luminescing)
Reaction(IUBMB)
a long-chain aldehyde + FMNH2 + O2 = a long-chain fatty acid + FMN + H2O + hnu [RN:
R10551 ]
Reaction(KEGG)
Substrate
Product
Comment
The reaction sequence starts with the incorporation of a molecule of oxygen into reduced FMN bound to the enzyme, forming luciferase peroxyflavin. The peroxyflavin interacts with an aliphatic long-chain aldehyde, producing a highly fluorescent species believed to be luciferase hydroxyflavin. The enzyme is highly specific for reduced FMN and for long-chain aliphatic aldehydes with eight carbons or more. The highest efficiency is achieved with tetradecanal. cf. EC
1.13.12.18 , dinoflagellate luciferase.
History
EC 1.14.14.3 created 1981, modified 2016
Orthology
K00494 alkanal monooxygenase alpha chain
K15854 alkanal monooxygenase beta chain
Genes
PLU : plu2081(luxA) plu2082(luxB)
PLUM : A4R40_10430 A4R40_10435
PAY : PAU_02511(luxB) PAU_02512(luxA)
PTT : VY86_20590 VY86_20595
PAKH : B0X70_10725 B0X70_10730
PLUI : CE143_10635 CE143_10640
VHA : VIBHAR_06241 VIBHAR_06242
VCA : M892_20835 M892_20840
VQI : CCZ37_17840 CCZ37_17845
VFI : VF_A0920(luxB) VF_A0921(luxA)
VFM : VFMJ11_A1038 VFMJ11_A1039
VSA : VSAL_II0961(luxB) VSAL_II0962(luxA)
ELUX : BTN50_1882 BTN50_1883
SHEM : HWQ47_10975 HWQ47_10980
IOD : EJO50_06705 EJO50_06710
» show all
Taxonomy
Reference
Authors
Hastings JW, Nealson KH.
Title
Bacterial bioluminescence.
Journal
Reference
Authors
Hastings JW.
Title
Bacterial bioluminescence light emission in the mixed function oxidation of reduced flavin and fatty aldehyde.
Journal
Reference
Authors
Hastings JW, Presswood RP.
Title
Bacterial luciferase: FMNH2-aldehyde oxidase.
Journal
Reference
Authors
Nealson KH, Hastings JW.
Title
Bacterial bioluminescence: its control and ecological significance.
Journal
Microbiol Rev 43:496-518 (1979)
Reference
5
Authors
Suzuki K, Kaidoh T, Katagiri M, Tsuchiya T.
Title
O2 incorporation into a long-chain fatty-acid during bacterial luminescence.
Journal
Biochim Biophys Acta 722:297-301 (1983)
Reference
Authors
Kurfurst M, Ghisla S, Hastings JW
Title
Characterization and postulated structure of the primary emitter in the bacterial luciferase reaction.
Journal
Other DBs
ExPASy - ENZYME nomenclature database: 1.14.14.3