KEGG   ENZYME: 1.14.14.30
Entry
EC 1.14.14.30               Enzyme                                 

Name
isobutylamine N-monooxygenase;
vlmH (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
2-methylpropan-1-amine,FADH2:O2 N-oxidoreductase
Reaction(IUBMB)
(1) 2-methylpropan-1-amine + FADH2 + O2 = N-(2-methylpropyl)hydroxylamine + FAD + H2O [RN:R11403];
(2) 2-methylpropan-1-amine + FMNH2 + O2 = N-(2-methylpropyl)hydroxylamine + FMN + H2O [RN:R11404]
Reaction(KEGG)
R11403 R11404
Substrate
2-methylpropan-1-amine [CPD:C02787];
FADH2 [CPD:C01352];
O2 [CPD:C00007];
FMNH2 [CPD:C01847]
Product
N-(2-methylpropyl)hydroxylamine [CPD:C21369];
FAD [CPD:C00016];
H2O [CPD:C00001];
FMN [CPD:C00061]
Comment
The enzyme, characterized from the bacterium Streptomyces viridifaciens, is part of a two component system that also includes a flavin reductase, which provides reduced flavin mononucleotide for this enzyme. The enzyme, which is involved in the biosynthesis of the azoxy antibiotic valanimycin, has a similar activity with either FMNH2 or FADH2. It exhibits broad specificity, and also accepts propan-1-amine, butan-1-amine, butan-2-amine and benzylamine.
History
EC 1.14.14.30 created 2016, modified 2017
Orthology
K21281  isobutylamine N-monooxygenase
Genes
KSK: KSE_09040(vlmH)
Reference
1  [PMID:9056232]
  Authors
Parry RJ, Li W
  Title
Purification and characterization of isobutylamine N-hydroxylase from the valanimycin producer Streptomyces viridifaciens MG456-hF10.
  Journal
Arch Biochem Biophys 339:47-54 (1997)
DOI:10.1006/abbi.1996.9857
  Sequence
Reference
2  [PMID:8990292]
  Authors
Parry RJ, Li W, Cooper HN
  Title
Cloning, analysis, and overexpression of the gene encoding isobutylamine N-hydroxylase from the valanimycin producer, Streptomyces viridifaciens.
  Journal
J Bacteriol 179:409-16 (1997)
DOI:10.1128/JB.179.2.409-416.1997
  Sequence
Reference
3  [PMID:9287340]
  Authors
Parry RJ, Li W
  Title
An NADPH:FAD oxidoreductase from the valanimycin producer, Streptomyces viridifaciens. Cloning, analysis, and overexpression.
  Journal
J Biol Chem 272:23303-11 (1997)
DOI:10.1074/jbc.272.37.23303
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.30
IUBMB Enzyme Nomenclature: 1.14.14.30
ExPASy - ENZYME nomenclature database: 1.14.14.30
BRENDA, the Enzyme Database: 1.14.14.30

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