Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy

(E)-phenylacetaldehyde oxime,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase

(E)-phenylacetaldehyde oxime + [reduced NADPH---hemoprotein reductase] + O2 = (R)-mandelonitrile + [oxidized NADPH---hemoprotein reductase] + 2 H2O (overall reaction) [RN:R11732];
(1a) (E)-phenylacetaldehyde oxime = (Z)-phenylacetaldehyde oxime [RN:R10041];
(1b) (Z)-phenylacetaldehyde oxime = phenylacetonitrile + H2O [RN:R07638];
(1c) phenylacetonitrile + [reduced NADPH---hemoprotein reductase] + O2 = (R)-mandelonitrile + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R11733]

R07638 R10041 R11732 R11733
Reaction

(E)-phenylacetaldehyde oxime [CPD:C19714];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007];
(Z)-phenylacetaldehyde oxime [CPD:C16075];
phenylacetonitrile [CPD:C16074]

This cytochrome P-450 (heme-thiolate) enzyme is involved in the biosynthesis of the cyanogenic glucosides (R)-prunasin and (R)-amygdalin. It catalyses three different activities - isomerization of the (E) isomer to the (Z) isomer, dehydration, and C-hydroxylation.

EC 1.14.14.44 created 2017

1  [PMID:25015725]