KEGG   ENZYME: 1.14.14.48Help
Entry
EC 1.14.14.48               Enzyme                                 

Name
jasmonoyl-L-amino acid 12-hydroxylase;
CYP94B1 (gene name);
CYP94B3 (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
jasmonoyl-L-amino acid,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (12-hydroxylating)
Reaction(IUBMB)
a jasmonoyl-L-amino acid + [reduced NADPH---hemoprotein reductase] + O2 = a 12-hydroxyjasmonoyl-L-amino acid + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R11806]
Reaction(KEGG)
Substrate
jasmonoyl-L-amino acid [CPD:C21509];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007]
Product
12-hydroxyjasmonoyl-L-amino acid [CPD:C21696];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001]
Comment
A cytochrome P450 (heme thiolate) enzyme found in plants. The enzyme acts on jasmonoyl-L-amino acid conjugates, catalysing the hydroxylation of the C-12 position of jasmonic acid. While the best studied substrate is (+)-7-epi-jasmonoyl-L-isoleucine, the enzyme was shown to be active with jasmonoyl-L-valine and jasmonoyl-L-phenylalanine, and is likely to be active with other jasmonoyl-amino acid conjugates.
History
EC 1.14.14.48 created 2017
Orthology
K20665  jasmonoyl-L-amino acid 12-hydroxylase
Genes
ATH: AT3G01900(CYP94B2) AT3G48520(CYP94B3) AT5G63450(CYP94B1)
ALY: ARALYDRAFT_485210 ARALYDRAFT_496532 ARALYDRAFT_896053
CRB: 17874658 17885442 17892234
CSAT: 104711346 104720538 104726960 104741179 104762484 104763703 104773500 104780667 104791069
EUS: EUTSA_v10005707mg EUTSA_v10011125mg
BRP: 103837470 103848967 103855044 103873137 103873527
BNA: 106351873 106364122 106382901 106405321 106409295 106428634 106430785 106431245 106443168 111208658 111213368
BOE: 106294052 106308148 106316769 106325388 106333823
THJ: 104818188 104821513 104822524
CPAP: 110807430 110820488
LJA: Lj0g3v0055949.1(Lj0g3v0055949.1) Lj0g3v0174429.1(Lj0g3v0174429.1) Lj0g3v0201059.1(Lj0g3v0201059.1)
ADU: 107494813
AIP: 107605023
LANG: 109344366
MCHA: 111007253
INI: 109171722
BVG: 104893484
SOE: 110787294
PSOM: 113302893
DOSA: Os11t0483000-00(Os11g0483000) Os12t0443000-00(Os12g0443000)
OBR: 102715166
BDI: 100823617
ATS: 109782849(LOC109782849)
SBI: 110435249
PDA: 103706541
EGU: 105051167
PEQ: 110018426
ATR: 18438139
 » show all
Taxonomy
Reference
1  [PMID:21576464]
  Authors
Koo AJ, Cooke TF, Howe GA
  Title
Cytochrome P450 CYP94B3 mediates catabolism and inactivation of the plant hormone jasmonoyl-L-isoleucine.
  Journal
Proc Natl Acad Sci U S A 108:9298-303 (2011)
DOI:10.1073/pnas.1103542108
  Sequence
[ath:AT3G48520]
Reference
2  [PMID:21849397]
  Authors
Kitaoka N, Matsubara T, Sato M, Takahashi K, Wakuta S, Kawaide H, Matsui H, Nabeta K, Matsuura H
  Title
Arabidopsis CYP94B3 encodes jasmonyl-L-isoleucine 12-hydroxylase, a key enzyme in the oxidative catabolism of jasmonate.
  Journal
Plant Cell Physiol 52:1757-65 (2011)
DOI:10.1093/pcp/pcr110
Reference
3  [PMID:22215670]
  Authors
Heitz T, Widemann E, Lugan R, Miesch L, Ullmann P, Desaubry L, Holder E, Grausem B, Kandel S, Miesch M, Werck-Reichhart D, Pinot F
  Title
Cytochromes P450 CYP94C1 and CYP94B3 catalyze two successive oxidation steps of plant hormone Jasmonoyl-isoleucine for catabolic turnover.
  Journal
J Biol Chem 287:6296-306 (2012)
DOI:10.1074/jbc.M111.316364
  Sequence
[ath:AT3G48520]
Reference
4  [PMID:24467969]
  Authors
Kitaoka N, Kawaide H, Amano N, Matsubara T, Nabeta K, Takahashi K, Matsuura H
  Title
CYP94B3 activity against jasmonic acid amino acid conjugates and the elucidation  of 12-O-beta-glucopyranosyl-jasmonoyl-L-isoleucine as an additional metabolite.
  Journal
Phytochemistry 99:6-13 (2014)
DOI:10.1016/j.phytochem.2013.12.019
Reference
5  [PMID:25210037]
  Authors
Koo AJ, Thireault C, Zemelis S, Poudel AN, Zhang T, Kitaoka N, Brandizzi F, Matsuura H, Howe GA
  Title
Endoplasmic reticulum-associated inactivation of the hormone jasmonoyl-L-isoleucine by multiple members of the cytochrome P450 94 family in Arabidopsis.
  Journal
J Biol Chem 289:29728-38 (2014)
DOI:10.1074/jbc.M114.603084
  Sequence
Reference
6  [PMID:26164240]
  Authors
Widemann E, Grausem B, Renault H, Pineau E, Heinrich C, Lugan R, Ullmann P, Miesch L, Aubert Y, Miesch M, Heitz T, Pinot F
  Title
Sequential oxidation of Jasmonoyl-Phenylalanine and Jasmonoyl-Isoleucine by multiple cytochrome P450 of the CYP94 family through newly identified aldehyde intermediates.
  Journal
Phytochemistry 117:388-99 (2015)
DOI:10.1016/j.phytochem.2015.06.027
  Sequence
[ath:AT3G48520]
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.48
IUBMB Enzyme Nomenclature: 1.14.14.48
ExPASy - ENZYME nomenclature database: 1.14.14.48
BRENDA, the Enzyme Database: 1.14.14.48

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