KEGG   ENZYME: 1.14.14.54Help
Entry
EC 1.14.14.54               Enzyme                                 

Name
phenylacetate 2-hydroxylase;
CYP504;
phaA (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
phenylacetate,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (2-hydroxylating)
Reaction(IUBMB)
phenylacetate + [reduced NADPH---hemoprotein reductase] + O2 = (2-hydroxyphenyl)acetate + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R05487]
Reaction(KEGG)
Substrate
phenylacetate [CPD:C07086];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007]
Product
2-hydroxyphenylacetate [CPD:C05852];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001]
Comment
This cytochrome P-450 (heme-thiolate) enzyme, found in Aspergillus nidulans, is involved in the degradation of phenylacetate.
History
EC 1.14.14.54 created 2017
Pathway
ec00360  Phenylalanine metabolism
ec00643  Styrene degradation
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K10437  phenylacetate 2-hydroxylase
Genes
QSU: 112006183 112014358
PPA: PAS_chr3_0864
DHA: DEHA2D03168g
PIC: PICST_49761
PGU: PGUG_01534 PGUG_05169
SPAA: SPAPADRAFT_48604
CAL: CAALFM_C404360WA(CaO19.1411)
CTP: CTRG_03930
COT: CORT_0E04490
CDU: CD36_44030
MGR: MGG_04684
CMT: CCM_02553
MBE: MBM_06099
ANI: AN8078.2
ANG: ANI_1_128144(An16g01030)
PCS: Pc21g14280(pahA)
ABE: ARB_03198
TVE: TRV_03913
 » show all
Taxonomy
Reference
1  [PMID:10329644]
  Authors
Mingot JM, Penalva MA, Fernandez-Canon JM
  Title
Disruption of phacA, an Aspergillus nidulans gene encoding a novel cytochrome P450 monooxygenase catalyzing phenylacetate 2-hydroxylation, results in penicillin overproduction.
  Journal
J Biol Chem 274:14545-50 (1999)
DOI:10.1074/jbc.274.21.14545
  Sequence
[ani:AN8078.2]
Reference
2  [PMID:11544206]
  Authors
Rodriguez-Saiz M, Barredo JL, Moreno MA, Fernandez-Canon JM, Penalva MA, Diez B
  Title
Reduced function of a phenylacetate-oxidizing cytochrome p450 caused strong genetic improvement in early phylogeny of penicillin-producing strains.
  Journal
J Bacteriol 183:5465-71 (2001)
DOI:10.1128/JB.183.19.5465-5471.2001
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.54
IUBMB Enzyme Nomenclature: 1.14.14.54
ExPASy - ENZYME nomenclature database: 1.14.14.54
BRENDA, the Enzyme Database: 1.14.14.54

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