Entry |
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Name |
taurochenodeoxycholate 6alpha-hydroxylase;
CYP3A4;
CYP4A21;
taurochenodeoxycholate 6alpha-monooxygenase
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Class |
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
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Sysname |
taurochenodeoxycholate,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (6alpha-hydroxylating)
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Reaction(IUBMB) |
(1) taurochenodeoxycholate + [reduced NADPH---hemoprotein reductase] + O2 = taurohyocholate + [oxidized NADPH---hemoprotein reductase] + H2O [RN: R07205];
(2) lithocholate + [reduced NADPH---hemoprotein reductase] + O2 = hyodeoxycholate + [oxidized NADPH---hemoprotein reductase] + H2O [RN: R07206]
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Reaction(KEGG) |
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Substrate |
taurochenodeoxycholate [CPD: C05465];
[reduced NADPH---hemoprotein reductase] [CPD: C03024];
O2 [CPD: C00007];
lithocholate [CPD: C03990]
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Product |
taurohyocholate [CPD: C15516];
[oxidized NADPH---hemoprotein reductase] [CPD: C03161];
H2O [CPD: C00001];
hyodeoxycholate [CPD: C15517]
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Comment |
A cytochrome P-450 (heme-thiolate) protein. Requires cytochrome b5 for maximal activity. Acts on taurochenodeoxycholate, taurodeoxycholate and less readily on lithocholate and chenodeoxycholate. In adult pig (Sus scrofa), hyocholic acid replaces cholic acid as a primary bile acid [5].
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History |
EC 1.14.14.57 created 2005 asEC 1.14.13.97, transferred 2018 to EC 1.14.14.57
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Orthology |
K17689 | cytochrome P450 family 3 subfamily A4 |
K18228 | taurochenodeoxycholate 6alpha-hydroxylase |
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Genes |
» show all
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Reference |
|
Authors |
Araya Z, Wikvall K. |
Title |
6alpha-hydroxylation of taurochenodeoxycholic acid and lithocholic acid by CYP3A4 in human liver microsomes. |
Journal |
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Sequence |
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Reference |
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Authors |
Araya Z, Hellman U, Hansson R. |
Title |
Characterisation of taurochenodeoxycholic acid 6 alpha-hydroxylase from pig liver microsomes. |
Journal |
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Sequence |
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Reference |
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Authors |
Kramer W, Sauber K, Baringhaus KH, Kurz M, Stengelin S, Lange G, Corsiero D, Girbig F, Konig W, Weyland C. |
Title |
Identification of the bile acid-binding site of the ileal lipid-binding protein by photoaffinity labeling, matrix-assisted laser desorption ionization-mass spectrometry, and NMR structure. |
Journal |
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Reference |
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Authors |
Lundell K, Hansson R, Wikvall K |
Title |
Cloning and expression of a pig liver taurochenodeoxycholic acid 6alpha-hydroxylase (CYP4A21): a novel member of the CYP4A subfamily. |
Journal |
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Sequence |
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Reference |
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Authors |
Lundell K, Wikvall K. |
Title |
Gene structure of pig sterol 12alpha-hydroxylase (CYP8B1) and expression in fetal liver: comparison with expression of taurochenodeoxycholic acid 6alpha-hydroxylase (CYP4A21). |
Journal |
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Reference |
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Authors |
Russell DW |
Title |
The enzymes, regulation, and genetics of bile acid synthesis. |
Journal |
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Other DBs |
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