KEGG   ENZYME: 1.14.14.61
Entry
EC 1.14.14.61               Enzyme                                 

Name
carnosic acid synthase;
CYP76AK6;
CYP76AK7;
CYP76AK8
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
11-hydroxyferruginol,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase
Reaction(IUBMB)
11-hydroxyferruginol + 3 [reduced NADPH---hemoprotein reductase] + 3 O2 = carnosic acid + 3 [oxidized NADPH---hemoprotein reductase] + 4 H2O [RN:R11973]
Reaction(KEGG)
R11973;
(other) R11989
Substrate
11-hydroxyferruginol [CPD:C21796];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007]
Product
carnosic acid [CPD:C21818];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001]
Comment
A cytochrome P-450 (heme-thiolate) protein isolated from the plants Salvia pomifera (apple sage), S. miltiorrhiza (red sage), S. fruticosa (Greek sage) and Rosmarinus officinalis (Rosemary).
History
EC 1.14.14.61 created 2018
Pathway
ec00904  Diterpenoid biosynthesis
ec01110  Biosynthesis of secondary metabolites
Orthology
K22638  carnosic acid synthase
Reference
1  [PMID:26976595]
  Authors
Ignea C, Athanasakoglou A, Ioannou E, Georgantea P, Trikka FA, Loupassaki S, Roussis V, Makris AM, Kampranis SC
  Title
Carnosic acid biosynthesis elucidated by a synthetic biology platform.
  Journal
Proc Natl Acad Sci U S A 113:3681-6 (2016)
DOI:10.1073/pnas.1523787113
  Sequence
Reference
2  [PMID:27703160]
  Authors
Scheler U, Brandt W, Porzel A, Rothe K, Manzano D, Bozic D, Papaefthimiou D, Balcke GU, Henning A, Lohse S, Marillonnet S, Kanellis AK, Ferrer A, Tissier A
  Title
Elucidation of the biosynthesis of carnosic acid and its reconstitution in yeast.
  Journal
Nat Commun 7:12942 (2016)
DOI:10.1038/ncomms12942
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.61
IUBMB Enzyme Nomenclature: 1.14.14.61
ExPASy - ENZYME nomenclature database: 1.14.14.61
BRENDA, the Enzyme Database: 1.14.14.61

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