KEGG   ENZYME: 1.14.14.63
Entry
EC 1.14.14.63               Enzyme                                 

Name
beta-amyrin 16beta-monooxygenase;
CYP716A141
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
beta-amyrin,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (maniladiol forming)
Reaction(IUBMB)
beta-amyrin + [reduced NADPH---hemoprotein reductase] + O2 = maniladiol + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R11975]
Reaction(KEGG)
R11975
Substrate
beta-amyrin [CPD:C08616];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007]
Product
maniladiol [CPD:C21820];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001]
Comment
A cytochrome P-450 (heme-thiolate) protein isolated from the plant Platycodon grandiflorus (baloon flower). The enzyme is also able to oxidize oleanolic acid to cochalic acid.
History
EC 1.14.14.63 created 2018
Orthology
K22620  beta-amyrin 16beta-monooxygenase
Genes
HAN: 110899122 110899124 110899149 110901577 110903161
LSV: 111881980
CCAV: 112499705
Reference
1  [PMID:28371833]
  Authors
Tamura K, Teranishi Y, Ueda S, Suzuki H, Kawano N, Yoshimatsu K, Saito K, Kawahara N, Muranaka T, Seki H
  Title
Cytochrome P450 Monooxygenase CYP716A141 is a Unique beta-Amyrin C-16beta Oxidase Involved in Triterpenoid Saponin Biosynthesis in Platycodon grandiflorus.
  Journal
Plant Cell Physiol 58:874-884 (2017)
DOI:10.1093/pcp/pcx043
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.63
IUBMB Enzyme Nomenclature: 1.14.14.63
ExPASy - ENZYME nomenclature database: 1.14.14.63
BRENDA, the Enzyme Database: 1.14.14.63

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