KEGG   ENZYME: 1.14.14.71
Entry
EC 1.14.14.71               Enzyme                                 

Name
cucurbitadienol 11-hydroxylase;
CYP87D18
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
cucurbitadienol,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (11-oxocucurbitadienol forming)
Reaction(IUBMB)
cucurbitadienol + 2 [reduced NADPH---hemoprotein reductase] + 2 O2 = 11-oxocucurbitadienol + 2 [oxidized NADPH---hemoprotein reductase] + 3 H2O (overall reaction) [RN:R12007];
(1a) cucurbitadienol + [reduced NADPH---hemoprotein reductase] + O2 = 11-hydroxycucurbitadienol + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R12008];
(1b) 11-hydroxycucurbitadienol + [reduced NADPH---hemoprotein reductase] + O2 = 11-oxocucurbitadienol + [oxidized NADPH---hemoprotein reductase] + 2 H2O [RN:R12009]
Reaction(KEGG)
Substrate
cucurbitadienol [CPD:C19819];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007];
11-hydroxycucurbitadienol [CPD:C21842]
Product
11-oxocucurbitadienol [CPD:C21841];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001];
11-hydroxycucurbitadienol [CPD:C21842]
Comment
Isolated from the plant Siraitia grosvenorii (monk fruit).
History
EC 1.14.14.71 created 2018
Reference
1  [PMID:26903528]
  Authors
Zhang J, Dai L, Yang J, Liu C, Men Y, Zeng Y, Cai Y, Zhu Y, Sun Y
  Title
Oxidation of Cucurbitadienol Catalyzed by CYP87D18 in the Biosynthesis of Mogrosides from Siraitia grosvenorii.
  Journal
Plant Cell Physiol 57:1000-7 (2016)
DOI:10.1093/pcp/pcw038
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.71
IUBMB Enzyme Nomenclature: 1.14.14.71
ExPASy - ENZYME nomenclature database: 1.14.14.71
BRENDA, the Enzyme Database: 1.14.14.71

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