KEGG   ENZYME: 1.14.14.76Help
Entry
EC 1.14.14.76               Enzyme                                 

Name
ent-isokaurene C2/C3-hydroxylase;
CYP71Z6;
ent-isokaurene C2-hydroxylase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
ent-isokaurene,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (ent-isokaurene-2beta,3beta-diol forming)
Reaction(IUBMB)
ent-isokaurene + 2 O2 + 2 [reduced NADPH---hemoprotein reductase] = ent-isokaurene-2beta,3beta-diol + [oxidized NADPH---hemoprotein reductase] + 2 H2O (overall reaction) [RN:R12058];
(1a) ent-isokaurene + O2 + [reduced NADPH---hemoprotein reductase] = ent-isokauren-2beta-ol + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R09861];
(1b) ent-isokauren-2beta-ol + O2 + [reduced NADPH---hemoprotein reductase] = ent-isokaurene-2beta,3beta-diol + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R11978]
Reaction(KEGG)
Substrate
ent-isokaurene [CPD:C20145];
O2 [CPD:C00007];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
ent-isokauren-2beta-ol [CPD:C20146]
Product
ent-isokaurene-2beta,3beta-diol [CPD:C21831];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001];
ent-isokauren-2beta-ol [CPD:C20146]
Comment
This cytochrome P-450 (heme thiolate) enzyme has been characterized from the plant Oryza sativa (rice). It may be involved in production of oryzadione.
History
EC 1.14.14.76 created 2012 as EC 1.14.13.143, transferred 2018 to EC 1.14.14.76
Pathway
ec00904  Diterpenoid biosynthesis
ec01110  Biosynthesis of secondary metabolites
Orthology
K16083  ent-isokaurene hydroxylase
Genes
OSA: 4329725
DOSA: Os02t0570500-01(Os02g0570500)
Taxonomy
Reference
1  [PMID:21985968]
  Authors
Wu Y, Hillwig ML, Wang Q, Peters RJ
  Title
Parsing a multifunctional biosynthetic gene cluster from rice: Biochemical characterization of CYP71Z6 & 7.
  Journal
FEBS Lett 585:3446-51 (2011)
DOI:10.1016/j.febslet.2011.09.038
  Sequence
[osa:4329725]
Reference
2  [PMID:25758958]
  Authors
Kitaoka N, Wu Y, Xu M, Peters RJ
  Title
Optimization of recombinant expression enables discovery of novel cytochrome P450 activity in rice diterpenoid biosynthesis.
  Journal
Appl Microbiol Biotechnol 99:7549-58 (2015)
DOI:10.1007/s00253-015-6496-2
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.76
IUBMB Enzyme Nomenclature: 1.14.14.76
ExPASy - ENZYME nomenclature database: 1.14.14.76
BRENDA, the Enzyme Database: 1.14.14.76

DBGET integrated database retrieval system