KEGG   ENZYME: 1.14.14.82Help
Entry
EC 1.14.14.82               Enzyme                                 

Name
flavonoid 3'-monooxygenase;
CYP75B1 (gene name);
flavonoid 3'-hydroxylase;
flavonoid 3-hydroxylase (incorrect);
NADPH:flavonoid-3'-hydroxylase (incorrect);
flavonoid 3-monooxygenase (incorrect)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
flavonoid,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (3'-hydroxylating)
Reaction(IUBMB)
a flavonoid + [reduced NADPH---hemoprotein reductase] + O2 = a 3'-hydroxyflavonoid + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R03615]
Reaction(KEGG)
Substrate
flavonoid [CPD:C01579];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007]
Product
3'-hydroxyflavonoid [CPD:C02790];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001]
Comment
A cytochrome P-450 (heme-thiolate) protein found in plants. Acts on a number of flavonoids, including the flavanone naringenin and the flavone apigenin. Does not act on 4-coumarate or 4-coumaroyl-CoA.
History
EC 1.14.14.82 created 1983 as EC 1.14.13.21, transferred 2018 to EC 1.14.14.82
Pathway
ec00941  Flavonoid biosynthesis
ec00944  Flavone and flavonol biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K05280  flavonoid 3'-monooxygenase
Genes
ATH: AT5G07990(TT7)
ALY: ARALYDRAFT_487632
CRB: 17881114
CSAT: 104708304 104734816 104769003
EUS: EUTSA_v10013289mg
BRP: 103847118
BNA: 106419056 111197716
BOE: 106313667
RSZ: 108814778
THJ: 104801243
CPAP: 110811144
CIT: 102620909
TCC: 18604820
GRA: 105764705
GAB: 108479810
VUN: 114164840
LJA: Lj1g3v2293820.1(Lj1g3v2293820.1) Lj4g3v1697590.1(Lj4g3v1697590.1) Lj4g3v1697590.2(Lj4g3v1697590.2) Lj4g3v1708650.1(Lj4g3v1708650.1) Lj4g3v2267360.1(Lj4g3v2267360.1)
ADU: 107471019
AIP: 107623580
RCN: 112178545
PPER: 18777306
PMUM: 103338591
PXB: 103930751
ZJU: 107419096
CSV: 101207160
CMO: 103494036
CMAX: 111499893
CMOS: 111440987
CPEP: 111792334
RCU: 8284440
JCU: 105640611
MESC: 110617654
JRE: 108982908
QSU: 112039462
VVI: 100232999(F3'H) 100263810(F3'H)
SLY: 101266618
SPEN: 107015315
SOT: 102593656
CANN: 107862334
NTA: 107773493(NtF3'H) 107795677(F3'H1) 107829530
NTO: 104093246
INI: 109160581(Magenta)
SIND: 105157984
OEU: 111401587
CCAV: 112514430
DCR: 108216209
BVG: 104907876
SOE: 110789886
DOSA: Os10t0317900-01(Os10g0317900) Os10t0320100-01(Os10g0320100)
ATS: 109737789(LOC109737789) 109737796(LOC109737796) 109767455(LOC109767455) 109773560(LOC109773560)
MUS: 103979831
ATR: 18428045
 » show all
Taxonomy
Reference
1
  Authors
Forkmann, G., Heller, W. and Grisebach, H.
  Title
Anthocyanin biosynthesis in flowers of Matthiola incana flavanone 3- and flavonoid 3'-hydroxylases.
  Journal
Z Naturforsch C: Biosci 35:691-695 (1980)
Reference
2  [PMID:10504566]
  Authors
Brugliera F, Barri-Rewell G, Holton TA, Mason JG
  Title
Isolation and characterization of a flavonoid 3'-hydroxylase cDNA clone corresponding to the Ht1 locus of Petunia hybrida.
  Journal
Plant J 19:441-51 (1999)
DOI:10.1046/j.1365-313X.1999.00539.x
  Sequence
Reference
3  [PMID:11030432]
  Authors
Schoenbohm C, Martens S, Eder C, Forkmann G, Weisshaar B
  Title
Identification of the Arabidopsis thaliana flavonoid 3'-hydroxylase gene and functional expression of the encoded P450 enzyme.
  Journal
Biol Chem 381:749-53 (2000)
DOI:10.1515/BC.2000.095
  Sequence
[ath:AT5G07990]
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.82
IUBMB Enzyme Nomenclature: 1.14.14.82
ExPASy - ENZYME nomenclature database: 1.14.14.82
BRENDA, the Enzyme Database: 1.14.14.82
CAS: 75991-44-5

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