Entry
Name
4-hydroxyphenylacetate 3-monooxygenase;
p-hydroxyphenylacetate 3-hydroxylase;
4-hydroxyphenylacetic acid-3-hydroxylase;
p-hydroxyphenylacetate hydroxylase (FAD);
4 HPA 3-hydroxylase;
p-hydroxyphenylacetate 3-hydroxylase (FAD);
HpaB
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
4-hydroxyphenylacetate,FADH2:oxygen oxidoreductase (3-hydroxylating)
Reaction(IUBMB)
4-hydroxyphenylacetate + FADH2 + O2 = 3,4-dihydroxyphenylacetate + FAD + H2O
Reaction(KEGG)
Substrate
Product
Comment
The enzyme from Escherichia coli attacks a broad spectrum of phenolic compounds. The enzyme uses FADH2 as a substrate rather than a cofactor [4]. FADH2 is provided by EC
1.5.1.36 , flavin reductase (NADH) [5,6].
History
EC 1.14.14.9 created 1972 as EC 1.14.13.3, transferred 2011 to EC 1.14.14.9
Pathway
ec01120 Microbial metabolism in diverse environments
Orthology
K00483 4-hydroxyphenylacetate 3-monooxygenase
K16901 anthranilate 3-monooxygenase (FAD) / 4-hydroxyphenylacetate 3-monooxygenase
K23492 4-hydroxyphenylacetate 3-monooxygenase
Genes
KOC : AB185_20685 AB185_32450
KGR : JJJ10_13550 JJJ10_24255(hpaB)
KPAS : LUW96_10280(hpaB) LUW96_21815
KLM : BWI76_03830 BWI76_15105
REE : electrica_04380(hpaB)
KLP : GUC22_12430 GUC22_23490(hpaB)
RTG : NCTC13098_06430(hpaB_2)
KIE : NCTC12125_02693(hpaB_1) NCTC12125_02694(hpaB_2)
YPG : YpAngola_A1640(hpaB)
YPI : YpsIP31758_2357(hpaB)
SFJ : SAMEA4384070_0661(hpaB)
SOF : NCTC11214_00922(hpaB)
SSAR : SSARUM_000557(hpaB)
SFO : Z042_02305 Z042_16840
PLU : plu0246 plu0975(hpaB) plu4027 plu4258
PLUM : A4R40_01205 A4R40_04870 A4R40_20005 A4R40_21115
PAY : PAU_00189(hpaB) PAU_00478 PAU_00910(hpaB)
PTT : VY86_09160 VY86_13810
PAKH : B0X70_01060 B0X70_02985 B0X70_05280
PLUI : CE143_01060 CE143_02970 CE143_05210(hpaB)
XBV : XBW1_1452(hpaB) XBW1_4467
XNE : XNC1_0446 XNC1_0449 XNC1_0811
XNM : XNC2_0437 XNC2_0439 XNC2_0797
XDO : XDD1_0415 XDD1_1013(hpaB)
XHO : A9255_13900 A9255_13930 A9255_20565
XBU : HGO23_05330(hpaB) HGO23_17980
XGR : QL128_15730(hpaB) QL128_19385
PHEI : NCTC12003_00816(hpaB)
PRJ : NCTC6933_00873(hpaB)
VNI : VIBNI_B1950(C2-hpah)
PAEB : NCGM1900_0869(hpaA)
PCQ : PcP3B5_43330(C2-hpah)
PPRC : PFLCHA0_c33860(hpaB)
PCH : EY04_16655 EY04_26305
PCP : JM49_05440 JM49_14085
PSEC : CCOS191_2761(C2-hpah)
PXN : HU772_012305 HU772_018355
PTK : EXN22_11260 EXN22_16475
PKK : QQ992_17495 QQ992_17655
EAZ : JHT90_12470(hpaB) JHT90_13905(hpaB)
ENTO : MTZ49_05110(hpaB) MTZ49_06570(hpaB)
KIM : G3T16_00445 G3T16_00495
GSN : YC6258_01805 YC6258_05643
RPJ : N234_22475 N234_29420
REH : H16_B0496(h16_B0496)
COX : E0W60_08660 E0W60_22670
BYI : BYI23_C002090 BYI23_C002900
BUE : BRPE67_CCDS09320 BRPE67_CCDS10130
BURT : BTHE68_55100 BTHE68_61140
CABK : NK8_53430 NK8_77800
BOJ : CBF45_15850 CBF45_15870
KGY : EHF36_15900(hpaB) EHF36_15920
SIX : BSY16_2360 BSY16_3678 BSY16_4601
AVF : RvVAR031_pl01430(hpaB)
BEL : BE61_64500(hpaB) BE61_86820
BBRA : QA636_26965 QA636_27310
BGK : IC762_14365 IC762_29510
VGO : GJW-30_1_02620(hpaB)
TRB : HB776_00730 HB776_12250 HB776_24575
XDI : EZH22_02415 EZH22_03590 EZH22_12875 EZH22_14710 EZH22_30930
STAR : G3545_06640 G3545_07185 G3545_20115 G3545_23450
LNE : FZC33_03830 FZC33_08785
APRA : G3A50_15685 G3A50_19050 G3A50_19060
AQI : J5J86_09760 J5J86_14865
RHZ : RHPLAN_16150 RHPLAN_17100 RHPLAN_22700 RHPLAN_32460 RHPLAN_51690 RHPLAN_54270 RHPLAN_62260
PLEO : OHA_1_00476 OHA_1_01081(hpaH) OHA_1_03164(npcA)
PSIN : CAK95_10695 CAK95_16885 CAK95_27280
NOH : G5V57_04365 G5V57_17175
PSTG : E8M01_14155 E8M01_21585 E8M01_25625
CEH : CEW89_08830 CEW89_12085
CON : TQ29_11625 TQ29_16040
SSTL : ABFK29_18150 ABFK29_23855
DAA : AKL17_0233 AKL17_3242 AKL17_3250
SRF : LHU95_08245 LHU95_08695
BAZ : BAMTA208_01065(yoaI)
BSTR : QI003_10090(hpaB) QI003_13520(hpaB)
BCEF : BcrFT9_01682 BcrFT9_02035
BTF : YBT020_12875 YBT020_29196
BTRO : FJR70_06330 FJR70_09265
BSM : BSM4216_0733 BSM4216_2177
BFD : NCTC4823_00762 NCTC4823_00782 NCTC4823_00810(hpaB) NCTC4823_01206 NCTC4823_02215(yoaI) NCTC4823_03859
BBAD : K7T73_02505(hpaB) K7T73_04805 K7T73_16300(hpaB) K7T73_21240
BACI : B1NLA3E_10730 B1NLA3E_17945 B1NLA3E_17950
BACO : OXB_2499 OXB_2686 OXB_3446 OXB_3454
BACA : FAY30_12455(hpaB) FAY30_13510 FAY30_13525
BHK : B4U37_04110 B4U37_13975
BMQ : BMQ_2417(hpaB) BMQ_3596(hpaB)
BMD : BMD_2382(hpaB) BMD_3581(hpaB)
BMEG : BG04_470 BG04_4791(hpaB)
BKO : CKF48_02440 CKF48_12865(hpaB)
GTE : GTCCBUS3UF5_24190 GTCCBUS3UF5_33960
GTK : GT3570_10150 GT3570_14800
BCAL : CWI35_12845(hpaB) CWI35_17760(hpaB)
GTN : GTNG_2992(hpaB) GTNG_3160
GMC : GY4MC1_0462 GY4MC1_1588
GGH : GHH_c22230(yoaI) GHH_c31080(hpaB)
GJF : M493_12230 M493_15810 M493_17955
GZA : IC807_06805(hpaB) IC807_11295(hpaB)
GTH : Geoth_0530 Geoth_1671
PTL : AOT13_00545 AOT13_14480
PCAL : BV455_00235(nphA1) BV455_02281
PARG : PspKH34_04420 PspKH34_15400
ANM : GFC28_1574(hpaB) GFC28_3725(hpaB)
ANL : GFC29_1708(hpaB) GFC29_54(hpaB)
AND : GRQ40_05030(hpaB) GRQ40_12980(hpaB)
LGY : T479_15720 T479_19670
LYS : LBYS11_11740 LBYS11_15360(hpaB)
LYB : C3943_11775(hpaB) C3943_22700
LYZ : DCE79_01715 DCE79_11215(hpaB)
LPAK : GDS87_16685(hpaB) GDS87_20255
LIU : OU989_11500 OU989_15325(hpaB)
LYQ : MY533_15015(hpaB) MY533_15270(hpaB) MY533_20315 MY533_20320
HHD : HBHAL_2383(hpaB) HBHAL_3970(yoaI)
HMN : HM131_07195 HM131_18375
HLI : HLI_17040(hpaB) HLI_19760(hpaB)
HNZ : P9989_02560 P9989_02620(hpaB) P9989_15065(hpaB)
HSAN : MUN89_08230(hpaB) MUN89_08795(hpaB)
HSHI : MUO14_04890(hpaB) MUO14_16460(hpaB)
VIL : CFK37_10560(hpaB) CFK37_10890(hpaB)
VSS : PD280_10675(hpaB) PD280_19965(hpaB)
FPN : ABE65_004705 ABE65_007020 ABE65_019825
FAR : ABE41_002760 ABE41_004850 ABE41_008075
FEC : QNH15_09405(hpaB) QNH15_22790
ACOP : RI196_07870 RI196_16905(hpaB)
BSJ : UP17_10485 UP17_15195 UP17_22410
BMUR : ABE28_014085 ABE28_020060
PASA : BAOM_0489 BAOM_2294(hpaB) BAOM_2295(hpaB)
PFRI : L8956_05235 L8956_11350 L8956_15915(hpaB)
PSYH : D0S48_01905(hpaB) D0S48_11000(hpaB)
PSYO : PB01_01290(hpaB) PB01_10745(hpaB) PB01_19030(hpaB)
RUE : DT065_12605 DT065_13750 DT065_14095(hpaB) DT065_14180 DT065_15175
PCHU : QNI29_02955(hpaB) QNI29_07240(hpaB)
NMK : CHR53_04775 CHR53_09955(hpaB) CHR53_10175(hpaB) CHR53_16415
NDT : L1999_03220(hpaB) L1999_17470
NCM : QNK12_02810 QNK12_05460 QNK12_10790 QNK12_11590(hpaB) QNK12_14400(hpaB)
NNV : QNH39_09970(hpaB) QNH39_24840
MSEM : GMB29_05745(hpaB) GMB29_05780 GMB29_08290 GMB29_22845(hpaB)
MENL : MVE64_08585(hpaB) MVE64_18475(hpaB)
BVJ : I5776_09945(hpaB) I5776_15190(hpaB)
BOU : I5818_04760 I5818_11355(hpaB) I5818_18885(hpaB)
HSPO : JGZ69_00765(hpaB) JGZ69_18045(hpaB)
SFOR : QNH23_06980(hpaB) QNH23_14325(hpaB)
ALKG : MOJ78_04850(hpaB) MOJ78_08055(hpaB)
HYI : K2M58_04775 K2M58_04815 K2M58_06415(hpaB) K2M58_09755
BHUI : LOK74_00560 LOK74_02335
BAYD : BSPP4475_07420(hpaB)
PBJ : VN24_08080 VN24_09465 VN24_09470 VN24_23030 VN24_23040
PNP : IJ22_02430 IJ22_35230 IJ22_35330 IJ22_36190 IJ22_40300 IJ22_40320 IJ22_47340 IJ22_47350
POW : IJ21_24840 IJ21_24850
PXL : BS614_17050 BS614_22065
PCHI : PC41400_28695(hpaB)
PCEL : HUB94_10130 HUB94_10140 HUB94_23490
PTHI : NDS46_14375(hpaB) NDS46_23260(hpaB)
PMAH : PTQ21_18440 PTQ21_25030
PAMY : P9222_14865 P9222_18215
PPAB : KET34_14640 KET34_21705
PEZZ : HW560_17915 HW560_24985
ANX : ACH33_11740 ACH33_14090
ASOC : CB4_02002(hpaH) CB4_02465
ATHE : K3F53_04820(hpaB) K3F53_07200
AUD : PO771_07515 PO771_09060(hpaB)
COHN : KCTCHS21_16060 KCTCHS21_49750
ACYC : JI721_10660 JI721_10970(hpaB)
KYR : CVV65_03310 CVV65_08580(hpaB) CVV65_10375
SOB : CSE16_08880(hpaB) CSE16_18810(hpaB)
SPSY : AZE41_13970 AZE41_14175
SPOS : DV702_11180 DV702_12005(hpaB)
SPAE : E2C16_06870 E2C16_12310
SOW : QWT69_13575(hpaB) QWT69_14260(hpaB)
PANC : E2636_09660(hpaB) E2636_17240(hpaB)
MYW : BVC93_00465 BVC93_19055 BVC93_26285
MTHN : 4412656_02473(C2-hpah)
MMON : EWR22_13885 EWR22_13940(hpaB)
NFA : NFA_20870 NFA_30560 NFA_43650
NFR : ERS450000_01009(hpaB_1) ERS450000_02023(hpaB_2) ERS450000_05550(hpaB_3)
RHA : RHA1_ro01782 RHA1_ro02380 RHA1_ro02514(pheA1) RHA1_ro03853 RHA1_ro03872 RHA1_ro08077
ROP : ROP_14550 ROP_20940 ROP_22410 ROP_37410(nphA1) ROP_pROB02-01900(hpaB)
ROA : Pd630_LPD00219 Pd630_LPD05941 Pd630_LPD06576 Pd630_LPD06741
RPY : Y013_12530 Y013_13560
RBY : CEJ39_00005 CEJ39_00940
RAV : AAT18_08140 AAT18_20190 AAT18_24410
RRZ : CS378_09635 CS378_13990 CS378_23155
RKO : JWS14_09035 JWS14_12385 JWS14_13195 JWS14_21190
ROZ : CBI38_27095 CBI38_27550 CBI38_30670
RPSK : JWS13_18995 JWS13_24135 JWS13_32395 JWS13_33455 JWS13_36975
RHOU : KZJ41_08705 KZJ41_21295 KZJ41_25865
RHOW : A4U64_00155 A4U64_04530 A4U64_16440
RHOJ : JVH1_17160 JVH1_19055 JVH1_24610 JVH1_25380 JVH1_28425
RHOS : QWW67_09960 QWW67_14890 QWW67_26030
RHOQ : QWW98_09965 QWW98_14895 QWW98_26055
RHOY : QNA09_05795 QNA09_17785 QNA09_21495
GJI : H1R19_06615 H1R19_07500
SLK : SLUN_13730 SLUN_27520
SPRA : CP972_28840 CP972_29130
SLAI : P8A22_03770 P8A22_34800
ACRY : AC20117_08920 AC20117_12975 AC20117_15235
ARTP : E5206_06020 E5206_09080(hpaB)
GPR : JQN66_14280 JQN66_16275
LMOI : VV02_01280 VV02_15335
AMQ : AMETH_1856 AMETH_5243
AMYC : CU254_12330 CU254_16930
AACD : LWP59_12455 LWP59_17440 LWP59_27055 LWP59_27200
ARHD : VSH64_05485 VSH64_30385
ACOR : LCL61_23125(hpaB) LCL61_30730
ACAB : QRX50_14440 QRX50_40860
PDX : Psed_0216 Psed_3087 Psed_6066
PBRO : HOP40_33130(hpaB) HOP40_33375(hpaB)
PPEL : H6H00_08535 H6H00_18695(hpaB)
SESP : BN6_55630 BN6_58660(sam5)
BALA : DSM104299_00957(hpaH)
SBAE : DSM104329_01674 DSM104329_03719(nphA1_1) DSM104329_03725(nphA1_2) DSM104329_03727(nphA1_3)
DMR : Deima_0220 Deima_1883
DEIN : DAAJ005_01865(hpaB)
TTJ : TTHA0960(TTHA0960) TTHB249(TTHB249)
MEMJ : MJ1HA_1089 MJ1HA_2028
MHK : DFR87_00965 DFR87_09780
MPRU : DFR88_01535 DFR88_09340
MTEN : GWK48_00105 GWK48_02510
MJN : MjAS7_1859 MjAS7_2927
» show all
Taxonomy
Reference
Authors
ADACHI K, TAKEDA Y, SENOH S, KITA H.
Title
METABOLISM OF P-HYDROXYPHENYLACETIC ACID IN PSEUDOMONAS OVALIS.
Journal
Reference
Authors
Prieto MA, Perez-Aranda A, Garcia JL
Title
Characterization of an Escherichia coli aromatic hydroxylase with a broad substrate range.
Journal
Reference
Authors
Prieto MA, Garcia JL
Title
Molecular characterization of 4-hydroxyphenylacetate 3-hydroxylase of Escherichia coli. A two-protein component enzyme.
Journal
J Biol Chem 269:22823-9 (1994)
Sequence
Reference
Authors
Xun L, Sandvik ER
Title
Characterization of 4-hydroxyphenylacetate 3-hydroxylase (HpaB) of Escherichia coli as a reduced flavin adenine dinucleotide-utilizing monooxygenase.
Journal
Sequence
Reference
Authors
Galan B, Diaz E, Prieto MA, Garcia JL
Title
Functional analysis of the small component of the 4-hydroxyphenylacetate 3-monooxygenase of Escherichia coli W: a prototype of a new Flavin:NAD(P)H reductase subfamily.
Journal
Reference
Authors
Louie TM, Xie XS, Xun L
Title
Coordinated production and utilization of FADH2 by NAD(P)H-flavin oxidoreductase and 4-hydroxyphenylacetate 3-monooxygenase.
Journal
Other DBs
ExPASy - ENZYME nomenclature database: 1.14.14.9
UM-BBD (Biocatalysis/Biodegradation Database): 1.14.14.9