KEGG   ENZYME: 1.14.14.90
Entry
EC 1.14.14.90               Enzyme                                 

Name
isoflavone 2'-hydroxylase;
isoflavone 2'-monooxygenase;
CYP81E1;
CYP Ge-3
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
isoflavone,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (2'-hydroxylating)
Reaction(IUBMB)
an isoflavone + [reduced NADPH---hemoprotein reductase] + O2 = a 2'-hydroxyisoflavone + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R07371]
Reaction(KEGG)
Substrate
isoflavone [CPD:C00799];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007]
Product
2'-hydroxyisoflavone [CPD:C02921];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001]
Comment
A cytochrome P-450 (heme-thiolate) protein. Acts on daidzein, formononetin and genistein. EC 1.14.14.89, 4'-methoxyisoflavone 2'-hydroxylase, has the same reaction but is more specific as it requires a 4'-methoxyisoflavone.
History
EC 1.14.14.90 created 2005 as EC 1.14.13.89, transferred 2018 to EC 1.14.14.90
Pathway
ec00943  Isoflavonoid biosynthesis
ec01110  Biosynthesis of secondary metabolites
Orthology
K13260  isoflavone/4'-methoxyisoflavone 2'-hydroxylase
Genes
CPAP: 110822377
CIT: 102624248
CIC: CICLE_v10025420mg
TCC: 18601853 18601857 18601860 18601861
GRA: 105763176 105789260
GHI: 107916731 107936806 107945765 107953970
GAB: 108470315 108476762
DZI: 111282012 111282131 111282224 111282978 111283036 111283932 111308417
EGR: 104420349
GMX: 100784650 100790507 100797371 100798269 100801092(CYP81E11) 100804509 100807819 100811727 100812102
GSJ: 114367022 114367290 114368609 114373520 114390631 114404224 114424679 114425468 114425469 114425838
PVU: PHAVU_002G014700g PHAVU_002G014800g PHAVU_002G187700g PHAVU_004G068400g PHAVU_009G244000g PHAVU_009G244100g PHAVU_009G244200g PHAVU_010G012900g PHAVU_010G013000g PHAVU_010G013100g
VRA: 106758033 106759715 106760052 106760053 106761763 106764429 106766213 106773653 106773682 106773765 106773766 106774281 106774974 106776630 106777449 106778042
VAR: 108319103 108321061 108321087 108321100 108321119 108321122 108334554 108335141 108343787 108344905 108345040 108346675 108346914 108347035 108347128
VUN: 114163099 114163100 114163781 114164214 114165712 114165763 114167228 114167561 114173717 114174265 114174626 114175001 114181477 114182143 114182199 114182463 114182619
CCAJ: 109790994 109792385 109793792 109794026 109794035 109806902 109811112
MTR: MTR_2g437840 MTR_4g094775 MTR_4g095050 MTR_5g016410 MTR_5g016440
CAM: 101489020 101502156(cyp81E3v2) 101502474(cyp81E3) 101502775 101503091 101505947 101506258(cyp81E2)
LJA: Lj0g3v0281229.2(Lj0g3v0281229.2) Lj2g3v1925640.1(Lj2g3v1925640.1) Lj4g3v0189840.1(Lj4g3v0189840.1) Lj4g3v0189850.1(Lj4g3v0189850.1)
ADU: 107480513 107482081 107483699 107487681 107487702 107487703 107487739
AIP: 107627314 107632124 107639093 107642734 107642736 107642737
LANG: 109329825 109331947 109334087 109334259 109345024 109352214 109353350 109353351 109353352 109357642
FVE: 101304511 101305403 101305693
RCN: 112184375 112186881 112190515
PPER: 18773681 18773715 18774146 18774496 18774581 18774650
PMUM: 103337225 103337235 103337245 103337257 103337275 103337298
PAVI: 110759579 110771616 110771619
MDM: 103401458 103432708 114821502
PXB: 103961611 103961612 103961613
ZJU: 107404429 107404433 107405743 107424869 107424872 107424876 107424878
CSV: 101210967 101211829 101215449
CMO: 103484810 103484812 103495911
MCHA: 111005286 111017882
CMAX: 111477798 111478140 111478736 111483621
CMOS: 111448607 111452969 111453889
CPEP: 111777333 111803141 111803665
RCU: 8285607 8285608
JCU: 105639782 105639783 105639784 105639785
HBR: 110645016 110668814
MESC: 110629129 110629218 110629283 110629407 110629531
POP: 18101164
JRE: 109007026 109007027 109010646
QSU: 112017242 112017252 112025873 112025878 112034317 112034350
VVI: 100249057 100250944 100259358 100261095
SLY: 101250559
SPEN: 107011466
SOT: 102601877
CANN: 107860230 107860232 107860235
NTA: 107782936
NSY: 104238470
NTO: 104120546
NAU: 109213256
INI: 109151908
SIND: 105162115 105162117
OEU: 111367220
DCR: 108196113
 » show all
Reference
1  [PMID:9790908]
  Authors
Akashi T, Aoki T, Ayabe S
  Title
CYP81E1, a cytochrome P450 cDNA of licorice (Glycyrrhiza echinata L.), encodes isoflavone 2'-hydroxylase.
  Journal
Biochem Biophys Res Commun 251:67-70 (1998)
DOI:10.1006/bbrc.1998.9414
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.90
IUBMB Enzyme Nomenclature: 1.14.14.90
ExPASy - ENZYME nomenclature database: 1.14.14.90
BRENDA, the Enzyme Database: 1.14.14.90
CAS: 110183-49-8

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Pathway (4)   
   KEGG PATHWAY (4)   
Chemical substance (16)   
   KEGG COMPOUND (16)   
Chemical reaction (7)   
   KEGG REACTION (6)   
   KEGG RCLASS (1)   
Gene (242)   
   KEGG ORTHOLOGY (1)   
   KEGG GENES (241)   
Protein sequence (4)   
   UniProt (3)   
   SWISS-PROT (1)   
Protein domain (4)   
   InterPro (4)   
All databases (277)   

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