KEGG   ENZYME: 1.14.14.90Help
Entry
EC 1.14.14.90               Enzyme                                 

Name
isoflavone 2'-hydroxylase;
isoflavone 2'-monooxygenase;
CYP81E1;
CYP Ge-3
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
isoflavone,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (2'-hydroxylating)
Reaction(IUBMB)
an isoflavone + [reduced NADPH---hemoprotein reductase] + O2 = a 2'-hydroxyisoflavone + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R07371]
Reaction(KEGG)
Substrate
isoflavone [CPD:C00799];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007]
Product
2'-hydroxyisoflavone [CPD:C02921];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001]
Comment
A cytochrome P-450 (heme-thiolate) protein. Acts on daidzein, formononetin and genistein. EC 1.14.14.89, 4'-methoxyisoflavone 2'-hydroxylase, has the same reaction but is more specific as it requires a 4'-methoxyisoflavone.
History
EC 1.14.14.90 created 2005 as EC 1.14.13.89, transferred 2018 to EC 1.14.14.90
Pathway
ec00943  Isoflavonoid biosynthesis
ec01110  Biosynthesis of secondary metabolites
Orthology
K13260  isoflavone/4'-methoxyisoflavone 2'-hydroxylase
Genes
CRB: 17888475
CSAT: 104710714 104713761 104716389 104716391 104719942 104721029 104729432 104729436 104729437 104750436
EUS: EUTSA_v10000659mg EUTSA_v10000720mg EUTSA_v10002362mg EUTSA_v10002364mg
BRP: 103842423 103854187
BNA: 106365171 106371131 106373964 106391411 106399661 106421608 106451545 111209880
BOE: 106306241 106324700
RSZ: 108809686 108809696 108811699 108851562
THJ: 104807607 104811160
CPAP: 110822257 110822446
CIT: 102624820
CIC: CICLE_v10027560mg
TCC: 18601858
GAB: 108456333
DZI: 111282012 111282978 111283036 111297590
EGR: 104420349 104420374 108957241
GMX: 100784650 100790507 100798269 100811727
GSJ: 114367290 114390631 114424679 114425838
PVU: PHAVU_002G187700g PHAVU_004G0682000 PHAVU_004G0682002 PHAVU_004G0683000 PHAVU_004G068400g PHAVU_009G244000g PHAVU_009G244100g PHAVU_009G244200g PHAVU_010G012900g PHAVU_010G013000g PHAVU_010G013100g
VRA: 106758033 106759715 106760052 106760053 106761763 106764429 106766213 106773653 106773682 106773765 106773766 106774281 106774974
VAR: 108319103 108321061 108321087 108321100 108321119 108321122 108343787 108344905 108345040 108346675 108346914 108347035 108347128
VUN: 114163099 114163100 114163781 114164214 114165712 114165763 114167228 114167561 114175001 114181477 114182143 114182199 114182463 114182619
CCAJ: 109790994 109792385 109792390 109793792 109794026 109794035 109794878 109806902 109813366
MTR: MTR_2g437840 MTR_4g094775 MTR_4g095050 MTR_5g016410
CAM: 101489020 101502156(cyp81E3v2) 101502474(cyp81E3) 101502775 101503091 101505947
LJA: Lj0g3v0281229.1(Lj0g3v0281229.1) Lj0g3v0281229.2(Lj0g3v0281229.2) Lj0g3v0281239.1(Lj0g3v0281239.1) Lj4g3v0189840.1(Lj4g3v0189840.1) Lj4g3v0189850.1(Lj4g3v0189850.1)
ADU: 107480513 107482081 107483699 107487681 107487702 107487703
AIP: 107627314 107632124 107639093 107642734 107642736
LANG: 109329825 109334087 109334259 109345024 109352214 109353350 109353351 109353352 109357642
FVE: 101305403 101315312
RCN: 112190707
PPER: 18773681 18773715 18774146 18774650
PMUM: 103337225 103337235 103337245 103337257 103337298
PAVI: 110771618
MDM: 114821502
PXB: 103961611 103961612 103961613
ZJU: 107404429 107404430 107404433 107405738 107424872 107424876 107424878 107433736 112488770
CSV: 101204413 101210967 101211829 101215449 101215922 101216167
CMO: 103484810 103484812 103495911 103495921
MCHA: 111008554 111017881 111017882 111017935
CMAX: 111477798 111478140 111478736 111481120 111483621
CMOS: 111443444 111452969 111453889
CPEP: 111777333 111787702 111803141 111803665
JCU: 105639782 105639786 110009166
HBR: 110643429 110663656 110672986
MESC: 110629283 110629407
POP: 18101164 7454262
PEU: 105142153
JRE: 109007026 109007027 109010646 109010647 109018593 109018594 109018595
QSU: 112015863 112017238 112017245 112017252 112025881 112034346 112034347 112034350 112034362
VVI: 100247722 100249138 100254242
SPEN: 114076841
CANN: 107867202 107867206
NTA: 107793695
NSY: 104238470
NAU: 109240371
INI: 109170795
SIND: 105162115 105162116 105162117 105162174 105177796 105177797
HAN: 110915326 110936025 110939925 110939927
CCAV: 112511745
DCR: 108217085 108217088 108219248
CQI: 110691472
 » show all
Taxonomy
Reference
1  [PMID:9790908]
  Authors
Akashi T, Aoki T, Ayabe S.
  Title
CYP81E1, a cytochrome P450 cDNA of licorice (Glycyrrhiza echinata L.), encodes isoflavone 2'-hydroxylase.
  Journal
Biochem Biophys Res Commun 251:67-70 (1998)
DOI:10.1006/bbrc.1998.9414
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.90
IUBMB Enzyme Nomenclature: 1.14.14.90
ExPASy - ENZYME nomenclature database: 1.14.14.90
BRENDA, the Enzyme Database: 1.14.14.90
CAS: 110183-49-8

  All links  
Pathway (4)   
   KEGG PATHWAY (4)   
Chemical substance (16)   
   KEGG COMPOUND (16)   
Chemical reaction (7)   
   KEGG REACTION (6)   
   KEGG RCLASS (1)   
Gene (255)   
   KEGG ORTHOLOGY (1)   
   KEGG GENES (254)   
Protein sequence (4)   
   UniProt (3)   
   SWISS-PROT (1)   
All databases (286)   

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