Entry |
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Name |
isoflavone 2'-hydroxylase;
isoflavone 2'-monooxygenase;
CYP81E1;
CYP Ge-3
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Class |
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
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Sysname |
isoflavone,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (2'-hydroxylating)
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Reaction(IUBMB) |
an isoflavone + [reduced NADPH---hemoprotein reductase] + O2 = a 2'-hydroxyisoflavone + [oxidized NADPH---hemoprotein reductase] + H2O [RN: R07371]
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Reaction(KEGG) |
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Substrate |
isoflavone [CPD: C00799];
[reduced NADPH---hemoprotein reductase] [CPD: C03024];
O2 [CPD: C00007]
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Product |
2'-hydroxyisoflavone [CPD: C02921];
[oxidized NADPH---hemoprotein reductase] [CPD: C03161];
H2O [CPD: C00001]
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Comment |
A cytochrome P-450 (heme-thiolate) protein. Acts on daidzein, formononetin and genistein. EC 1.14.14.89, 4'-methoxyisoflavone 2'-hydroxylase, has the same reaction but is more specific as it requires a 4'-methoxyisoflavone.
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History |
EC 1.14.14.90 created 2005 as EC 1.14.13.89, transferred 2018 to EC 1.14.14.90
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Pathway |
ec01110 | Biosynthesis of secondary metabolites |
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Orthology |
K13260 | isoflavone/4'-methoxyisoflavone 2'-hydroxylase |
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Genes |
» show all
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Reference |
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Authors |
Akashi T, Aoki T, Ayabe S. |
Title |
CYP81E1, a cytochrome P450 cDNA of licorice (Glycyrrhiza echinata L.), encodes isoflavone 2'-hydroxylase. |
Journal |
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Sequence |
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Other DBs |
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