KEGG   ENZYME: 1.14.14.90Help
Entry
EC 1.14.14.90               Enzyme                                 

Name
isoflavone 2'-hydroxylase;
isoflavone 2'-monooxygenase;
CYP81E1;
CYP Ge-3
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
isoflavone,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (2'-hydroxylating)
Reaction(IUBMB)
an isoflavone + [reduced NADPH---hemoprotein reductase] + O2 = a 2'-hydroxyisoflavone + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R07371]
Reaction(KEGG)
Substrate
isoflavone [CPD:C00799];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007]
Product
2'-hydroxyisoflavone [CPD:C02921];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001]
Comment
A cytochrome P-450 (heme-thiolate) protein. Acts on daidzein, formononetin and genistein. EC 1.14.14.89, 4'-methoxyisoflavone 2'-hydroxylase, has the same reaction but is more specific as it requires a 4'-methoxyisoflavone.
History
EC 1.14.14.90 created 2005 as EC 1.14.13.89, transferred 2018 to EC 1.14.14.90
Pathway
ec00943  Isoflavonoid biosynthesis
ec01110  Biosynthesis of secondary metabolites
Orthology
K13260  isoflavone/4'-methoxyisoflavone 2'-hydroxylase
Genes
CRB: 17888475
CSAT: 104710714 104713761 104716389 104716391 104719942 104721029 104729432 104729436 104729437 104750436
EUS: EUTSA_v10000659mg EUTSA_v10000720mg EUTSA_v10002362mg EUTSA_v10002364mg
BRP: 103842423 103854187
BNA: 106365171 106371131 106373964 106391411 106399661 106421608 106451545 111209880
BOE: 106306241 106324700
RSZ: 108809686 108809696 108811699 108851562
THJ: 104807607 104811160
CPAP: 110822257 110822446
CIT: 102624820
TCC: 18601858
GAB: 108456333
LJA: Lj0g3v0281229.1(Lj0g3v0281229.1) Lj0g3v0281229.2(Lj0g3v0281229.2) Lj0g3v0281239.1(Lj0g3v0281239.1) Lj4g3v0189840.1(Lj4g3v0189840.1) Lj4g3v0189850.1(Lj4g3v0189850.1)
RCN: 112190707
PAVI: 110771618
MDM: 114821502
PEU: 105142153
SPEN: 114076841
NTA: 107793695
NSY: 104238470
NAU: 109240371
INI: 109170795
CCAV: 112511745
CQI: 110691472
 » show all
Taxonomy
Reference
1  [PMID:9790908]
  Authors
Akashi T, Aoki T, Ayabe S.
  Title
CYP81E1, a cytochrome P450 cDNA of licorice (Glycyrrhiza echinata L.), encodes isoflavone 2'-hydroxylase.
  Journal
Biochem Biophys Res Commun 251:67-70 (1998)
DOI:10.1006/bbrc.1998.9414
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.90
IUBMB Enzyme Nomenclature: 1.14.14.90
ExPASy - ENZYME nomenclature database: 1.14.14.90
BRENDA, the Enzyme Database: 1.14.14.90
CAS: 110183-49-8

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