KEGG   ENZYME: 1.14.14.94
Entry
EC 1.14.14.94               Enzyme                                 

Name
leukotriene-B4 20-monooxygenase;
leukotriene-B4 20-hydroxylase;
leucotriene-B4 omega-hydroxylase;
LTB4 20-hydroxylase;
LTB4 omega-hydroxylase;
CYP4F2 (gene name);
CYP4F3 (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
(6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-6,8,10,14-tetraenoate,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (20-hydroxylating)
Reaction(IUBMB)
(6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-6,8,10,14-tetraenoate + [reduced NADPH---hemoprotein reductase] + O2 = (6Z,8E,10E,14Z)-(5S,12R)-5,12,20-trihydroxyicosa-6,8,10,14-tetraenoate + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R03866]
Reaction(KEGG)
R03866
Substrate
(6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-6,8,10,14-tetraenoate [CPD:C02165];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007]
Product
(6Z,8E,10E,14Z)-(5S,12R)-5,12,20-trihydroxyicosa-6,8,10,14-tetraenoate [CPD:C04853];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001]
Comment
A cytochrome P-450 (heme-thiolate) protein found in mammals.
History
EC 1.14.14.94 created 1989 as EC 1.14.13.30, transferred 2018 to EC 1.14.14.94
Pathway
ec00590  Arachidonic acid metabolism
Orthology
K17726  phylloquinone omega-hydroxylase / docosahexaenoic acid omega-hydroxylase / leukotriene-B4 20-monooxygenase
Genes
HSA: 4051(CYP4F3) 8529(CYP4F2)
PTR: 100609185 748117(CYP4F3) 748531(CYP4F2)
PPS: 100967411 100969141 100969847 100982619
GGO: 101125692 101135418 101152881
PON: 100434096 100437847 100453229
NLE: 100579320 100588006 115836968
MCC: 718349 719317
MCF: 102130440(CYP4F3v2) 102134301(CYP4F45)
CSAB: 103234079 103234089
RRO: 104661012 104661014
CJC: 100411160 100412575(CYP4F2)
TUP: 102483333
DRO: 112305042
 » show all
Reference
1  [PMID:3027095]
  Authors
Romano MC, Eckardt RD, Bender PE, Leonard TB, Straub KM, Newton JF.
  Title
Biochemical characterization of hepatic microsomal leukotriene B4 hydroxylases.
  Journal
J Biol Chem 262:1590-5 (1987)
Reference
2  [PMID:4044832]
  Authors
Shak S, Goldstein IM.
  Title
Leukotriene B4 omega-hydroxylase in human polymorphonuclear leukocytes. Partial purification and identification as a cytochrome P-450.
  Journal
J Clin Invest 76:1218-28 (1985)
DOI:10.1172/JCI112077
Reference
3  [PMID:2986111]
  Authors
Soberman RJ, Harper TW, Murphy RC, Austen KF.
  Title
Identification and functional characterization of leukotriene B4 20-hydroxylase of human polymorphonuclear leukocytes.
  Journal
Proc Natl Acad Sci U S A 82:2292-5 (1985)
DOI:10.1073/pnas.82.8.2292
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.94
IUBMB Enzyme Nomenclature: 1.14.14.94
ExPASy - ENZYME nomenclature database: 1.14.14.94
BRENDA, the Enzyme Database: 1.14.14.94
CAS: 90119-11-2

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