Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor

(+)-germacrene-A,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (12-hydroxylating)

(+)-germacrene A + 3 [reduced NADPH---hemoprotein reductase] + 3 O2 = germacra-1(10),4,11(13)-trien-12-oate + 3 [oxidized NADPH---hemoprotein reductase] + 4 H2O (overall reaction) [RN:R12128];
(1a) (+)-germacrene A + O2 + [reduced NADPH---hemoprotein reductase] = germacra-1(10),4,11(13)-trien-12-ol + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R09549];
(1b) germacra-1(10),4,11(13)-trien-12-ol + O2 + [reduced NADPH---hemoprotein reductase] = germacra-1(10),4,11(13)-trien-12-al + [oxidized NADPH---hemoprotein reductase] + 2 H2O [RN:R12129];
(1c) germacra-1(10),4,11(13)-trien-12-al + O2 + [reduced NADPH---hemoprotein reductase] = germacra-1(10),4,11(13)-trien-12-oate + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R12130]

R09549 R12128 R12129 R12130;
(other) R09548

(+)-germacrene A [CPD:C16141];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007];
germacra-1(10),4,11(13)-trien-12-ol [CPD:C19676];
germacra-1(10),4,11(13)-trien-12-al [CPD:C19677]

A cytochrome P-450 (heme-thiolate) protein. This plant enzyme catalyses three steps in a pathway that leads to the biosynthesis of many sesquiterpenoid lactones.

EC 1.14.14.95 created 2011 as EC 1.14.13.123, transferred 2018 to EC 1.14.14.95

1  [PMID:20351109]

Nguyen DT, Gopfert JC, Ikezawa N, Macnevin G, Kathiresan M, Conrad J, Spring O, Ro DK

Biochemical conservation and evolution of germacrene A oxidase in asteraceae.

J Biol Chem 285:16588-98 (2010)
DOI:10.1074/jbc.M110.111757

[ag:ADF43080 ADF32078]

2  [PMID:24704560]

Liu Q, Manzano D, Tanic N, Pesic M, Bankovic J, Pateraki I, Ricard L, Ferrer A, de Vos R, van de Krol S, Bouwmeester H

Elucidation and in planta reconstitution of the parthenolide biosynthetic pathway.

Metab Eng 23:145-53 (2014)
DOI:10.1016/j.ymben.2014.03.005