Entry
Name
camphor 5-monooxygenase;
camphor 5-exo-methylene hydroxylase;
2-bornanone 5-exo-hydroxylase;
bornanone 5-exo-hydroxylase;
camphor 5-exo-hydroxylase;
camphor 5-exohydroxylase;
camphor hydroxylase;
d-camphor monooxygenase;
methylene hydroxylase;
methylene monooxygenase;
D-camphor-exo-hydroxylase;
camphor methylene hydroxylase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
(+)-camphor,reduced putidaredoxin:oxygen oxidoreductase (5-hydroxylating)
Reaction(IUBMB)
(+)-camphor + reduced putidaredoxin + O2 = (+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O [RN:
R03818 ]
Reaction(KEGG)
Substrate
Product
(+)-exo-5-hydroxycamphor [CPD:
C03448 ];
oxidized putidaredoxin [CPD:
C03302 ];
H2O [CPD:
C00001 ]
Comment
A heme-thiolate protein (P-450). Also acts on (-)-camphor and 1,2-campholide, forming 5-exo-hydroxy-1,2-campholide.
History
EC 1.14.15.1 created 1972, modified 1986
Pathway
ec00907 Pinene, camphor and geraniol degradation
ec01120 Microbial metabolism in diverse environments
Orthology
K21569 camphor 5-monooxygenase
Genes
SPOG : EEB18_005500 EEB18_007630 EEB18_007765
SPYF : EAO27_03140 EAO27_06110 EAO27_09420 EAO27_13100
Taxonomy
Reference
Authors
Hedegaard J, Gunsalus IC.
Title
Mixed function oxidation. IV. An induced methylene hydroxylase in camphor oxidation.
Journal
J Biol Chem 240:4038-43 (1965)
Reference
Authors
Tyson CA, Lipscomb JD, Gunsalus IC.
Title
The role of putidaredoxin and P450 cam in methylene hydroxylation.
Journal
J Biol Chem 247:5777-84 (1972)
Other DBs
ExPASy - ENZYME nomenclature database: 1.14.15.1
UM-BBD (Biocatalysis/Biodegradation Database): 1.14.15.1