KEGG   ENZYME: 1.14.15.13
Entry
EC 1.14.15.13               Enzyme                                 
Name
pulcherriminic acid synthase;
cyclo-L-leucyl-L-leucyl dipeptide oxidase;
CYP134A1;
CypX (ambiguous)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
cyclo(L-leucyl-L-leucyl),reduced-ferredoxin:oxygen oxidoreductase (N-hydroxylating,aromatizing)
Reaction(IUBMB)
cyclo(L-leucyl-L-leucyl) + 6 reduced ferredoxin + 3 O2 = pulcherriminic acid + 6 oxidized ferredoxin + 4 H2O [RN:R10322]
Reaction(KEGG)
R10322
Substrate
cyclo(L-leucyl-L-leucyl) [CPD:C20514];
reduced ferredoxin [CPD:C00138];
O2 [CPD:C00007]
Product
pulcherriminic acid [CPD:C20515];
oxidized ferredoxin [CPD:C00139];
H2O [CPD:C00001]
Comment
A heme-thiolate (P-450) enzyme from the bacterium Bacillus subtilis. The order of events during the overall reaction is unknown. Pulcherrimic acid spontaneously forms an iron chelate with Fe(3+) to form the red pigment pulcherrimin [2].
History
EC 1.14.15.13 created 2013
Orthology
K17474  pulcherriminic acid synthase
Genes
PLUplu0296
PLUMA4R40_01445
PAKHB0X70_01350
PLUICE143_01350
MMEMarme_0277
BSUBSU35060(cypX)
BSHBSU6051_35060(cypX)
BSUTBSUB_03736(prfB)
BSULBSUA_03736(prfB)
BSUSQ433_19190
BSQB657_35060(cypX)
BSPU712_17560
BSSBSUW23_17185(cypX)
BSTGYO_3843
BLIBL00818(cypX)
BLDBLi03567(cypX)
BLHBaLi_c36290(cypX)
BMOJHC660_33910(cypX)
BBYCY96_28935
BACLBS34A_38160(cypX)
BALMBsLM_3513
BITBIS30_07155
BGIBGM20_11660
ACAIISX45_01295
SEPSDP17_2386
SHApSHaeC07
SSDSPSINT_1707
SDTSPSE_0788(cypX)
SDPNCTC12225_00488(cypX)
STRMM444_03610
SCZABE83_28615
SGSAVL59_18625
SLXSLAV_36095(cypX2)
SLKSLUN_30550
SVNCP980_02950
SCHACP983_42200
SNFJYK04_00990(cypX)
KABB7C62_06170
 » show all
Reference
1  [PMID:5837792]
  Authors
MacDonald JC
  Title
Biosynthesis of pulcherriminic acid.
  Journal
Biochem J 96:533-8 (1965)
DOI:10.1042/bj0960533
Reference
2  [PMID:20690619]
  Authors
Cryle MJ, Bell SG, Schlichting I
  Title
Structural and biochemical characterization of the cytochrome P450 CypX (CYP134A1) from Bacillus subtilis: a cyclo-L-leucyl-L-leucyl dipeptide oxidase.
  Journal
Biochemistry 49:7282-96 (2010)
DOI:10.1021/bi100910y
  Sequence
[bsu:BSU35060]
Other DBs
ExplorEnz - The Enzyme Database: 1.14.15.13
IUBMB Enzyme Nomenclature: 1.14.15.13
ExPASy - ENZYME nomenclature database: 1.14.15.13
BRENDA, the Enzyme Database: 1.14.15.13

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