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Entry
EC 1.14.15.13               Enzyme                                 

Name
pulcherriminic acid synthase;
cyclo-L-leucyl-L-leucyl dipeptide oxidase;
CYP134A1;
CypX (ambiguous)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
cyclo(L-leucyl-L-leucyl),reduced-ferredoxin:oxygen oxidoreductase (N-hydroxylating,aromatizing)
Reaction(IUBMB)
cyclo(L-leucyl-L-leucyl) + 6 reduced ferredoxin + 3 O2 = pulcherriminic acid + 6 oxidized ferredoxin + 4 H2O [RN:R10322]
Reaction(KEGG)
Substrate
cyclo(L-leucyl-L-leucyl) [CPD:C20514];
reduced ferredoxin [CPD:C00138];
O2 [CPD:C00007]
Product
pulcherriminic acid [CPD:C20515];
oxidized ferredoxin [CPD:C00139];
H2O [CPD:C00001]
Comment
A heme-thiolate (P-450) enzyme from the bacterium Bacillus subtilis. The order of events during the overall reaction is unknown. Pulcherrimic acid spontaneously forms an iron chelate with Fe(3+) to form the red pigment pulcherrimin [2].
History
EC 1.14.15.13 created 2013
Orthology
K17474  pulcherriminic acid synthase
Genes
PLU: plu0296
MME: Marme_0277
BSU: BSU35060(cypX)
BSH: BSU6051_35060(cypX)
BSUT: BSUB_03736(prfB)
BSUL: BSUA_03736(prfB)
BSUS: Q433_19190
BSS: BSUW23_17185(cypX)
BST: GYO_3843
BSQ: B657_35060(cypX)
BLI: BL00818(cypX)
BLD: BLi03567(cypX)
BLH: BaLi_c36290(cypX)
BACL: BS34A_38160(cypX)
BALM: BsLM_3513
SEPS: DP17_2386
SHA: pSHaeC07
SDT: SPSE_0788(cypX)
PDX: Psed_2589
 » show all
Taxonomy
Reference
1  [PMID:5837792]
  Authors
MacDonald JC
  Title
Biosynthesis of pulcherriminic acid.
  Journal
Biochem J 96:533-8 (1965)
Reference
2  [PMID:20690619]
  Authors
Cryle MJ, Bell SG, Schlichting I
  Title
Structural and biochemical characterization of the cytochrome P450 CypX (CYP134A1) from Bacillus subtilis: a cyclo-L-leucyl-L-leucyl dipeptide oxidase.
  Journal
Biochemistry 49:7282-96 (2010)
DOI:10.1021/bi100910y
  Sequence
[bsu:BSU35060]
Other DBs
ExplorEnz - The Enzyme Database: 1.14.15.13
IUBMB Enzyme Nomenclature: 1.14.15.13
ExPASy - ENZYME nomenclature database: 1.14.15.13
BRENDA, the Enzyme Database: 1.14.15.13

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