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Entry
EC 1.14.15.14               Enzyme                                 

Name
methyl-branched lipid omega-hydroxylase;
CYP124
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
methyl-branched lipid,reduced-ferredoxin:oxygen oxidoreductase (omega-hydroxylating)
Reaction(IUBMB)
a methyl-branched lipid + O2 + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ = an omega-hydroxy-methyl-branched lipid + H2O + 2 oxidized ferredoxin [iron-sulfur] cluster
Substrate
methyl-branched lipid;
O2 [CPD:C00007];
reduced ferredoxin [iron-sulfur] cluster [CPD:C00138];
H+ [CPD:C00080]
Product
omega-hydroxy-methyl-branched lipid;
H2O [CPD:C00001];
oxidized ferredoxin [iron-sulfur] cluster [CPD:C00139]
Comment
The enzyme, found in pathogenic and nonpathogenic mycobacteria species, actinomycetes, and some proteobacteria, hydroxylates the omega-carbon of a number of methyl-branched lipids, including (2E,6E)-farnesol, phytanate, geranylgeraniol, 15-methylpalmitate and (2E,6E)-farnesyl diphosphate. It is a P-450 heme-thiolate enzyme.
History
EC 1.14.15.14 created 2015
Orthology
K20497  methyl-branched lipid omega-hydroxylase
Genes
SCL: sce7867
SCU: SCE1572_45270
NGL: RG1141_PB00370
OTM: OSB_05620 OSB_15900
NTD: EGO55_14220
MTU: Rv2266(cyp124)
MTV: RVBD_2266
MTC: MT2328
MRA: MRA_2286(cyp124)
MTF: TBFG_12293
MTUR: CFBS_2399(cyp124)
MTO: MTCTRI2_2301(cyp124)
MTD: UDA_2266(cyp124)
MTN: ERDMAN_2490(cyp124)
MTUB: MT7199_2297(cyp124)
MTUC: J113_15800
MTUE: J114_12155
MTUH: I917_15990
MTUL: TBHG_02216
MTUT: HKBT1_2390(cyp124)
MTUU: HKBT2_2392(cyp124)
MTQ: HKBS1_2396(cyp124)
MBO: BQ2027_MB2289(cyp124)
MBB: BCG_2283(cyp124)
MBT: JTY_2277(cyp124)
MBM: BCGMEX_2271(cyp124)
MBX: BCGT_2089
MAF: MAF_22760(cyp124)
MMIC: RN08_2514
MCE: MCAN_22901(cyp124)
MCQ: BN44_50212(cyp)
MCV: BN43_31508(cyp)
MCX: BN42_40188(cyp)
MCZ: BN45_50597(cyp)
MPA: MAP_2015
MAO: MAP4_1810
MAVI: RC58_09040
MAVU: RE97_09045
MAV: MAV_2174
MUL: MUL_1282(cyp124A1)
MMC: Mmcs_2742
MKM: Mkms_2786
MJL: Mjls_2772
MMI: MMAR_3361(cyp124A1)
MMAE: MMARE11_32610(cyp124A1) MMARE11_36680(cyp268A2)
MLI: MULP_03625(cyp124A1) MULP_04016(cyp268A2)
MHAD: B586_09820
MSG: MSMEI_3470(cyp124) MSMEI_4503
MVA: Mvan_3029
MGI: Mflv_3307
MVQ: MYVA_3042
MJD: JDM601_1471(cyp268A2)
MTER: 4434518_01367(cyp268A2_1)
RER: RER_07870
ROP: ROP_71490
SGB: WQO_32195
SFA: Sfla_5961
SBH: SBI_08399(nanP)
STRP: F750_0609
SFI: SFUL_6845
STRM: M444_15025
SPRI: SPRI_2879
SMAL: SMALA_7272
KSK: KSE_61620
TCU: Tcur_1580
SRO: Sros_7140
FAL: FRAAL3377
BSD: BLASA_4033(cyp)
MMAR: MODMU_4611(cyp)
PDX: Psed_5473
PSEA: WY02_09315
PSEH: XF36_01040
ACTN: L083_3648
CAI: Caci_0813
TBI: Tbis_2431
 » show all
Taxonomy
Reference
1  [PMID:19933331]
  Authors
Johnston JB, Kells PM, Podust LM, Ortiz de Montellano PR
  Title
Biochemical and structural characterization of CYP124: a methyl-branched lipid omega-hydroxylase from Mycobacterium tuberculosis.
  Journal
Proc Natl Acad Sci U S A 106:20687-92 (2009)
DOI:10.1073/pnas.0907398106
  Sequence
[mtu:Rv2266]
Other DBs
ExplorEnz - The Enzyme Database: 1.14.15.14
IUBMB Enzyme Nomenclature: 1.14.15.14
ExPASy - ENZYME nomenclature database: 1.14.15.14
BRENDA, the Enzyme Database: 1.14.15.14

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