KEGG   ENZYME: 1.14.15.18
Entry
EC 1.14.15.18               Enzyme                                 
Name
calcidiol 1-monooxygenase;
25-hydroxycholecalciferol 1-hydroxylase;
25-hydroxycholecalciferol 1-monooxygenase;
1-hydroxylase-25-hydroxyvitamin D3;
25-hydroxy D3-1alpha-hydroxylase;
25-hydroxycholecalciferol 1alpha-hydroxylase;
25-hydroxyvitamin D3 1alpha-hydroxylase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
calcidiol,adrenodoxin:oxygen oxidoreductase (1-hydroxylating)
Reaction(IUBMB)
(1) calcidiol + 2 reduced adrenodoxin + 2 H+ + O2 = calcitriol + 2 oxidized adrenodoxin + H2O [RN:R03610];
(2) secalciferol + 2 reduced adrenodoxin + 2 H+ + O2 = calcitetrol + 2 oxidized adrenodoxin + H2O [RN:R11324]
Reaction(KEGG)
R03610 R11324
Substrate
calcidiol [CPD:C01561];
reduced adrenodoxin [CPD:C00662];
H+ [CPD:C00080];
O2 [CPD:C00007];
secalciferol [CPD:C07712]
Product
calcitriol [CPD:C01673];
oxidized adrenodoxin [CPD:C00667];
H2O [CPD:C00001];
calcitetrol [CPD:C18231]
Comment
A P-450 (heme-thiolate) enzyme found in mammals.
History
EC 1.14.15.18 created 1976 as EC 1.14.13.13, transferred 2016 to EC 1.14.15.18
Pathway
ec00100  Steroid biosynthesis
ec01100  Metabolic pathways
Orthology
K07438  calcidiol 1-monooxygenase (25-hydroxyvitamin D3 1alpha-hydroxylase)
Genes
HSA1594(CYP27B1)
PTR452027(CYP27B1)
PPS100974264(CYP27B1)
GGO101146696
PON100443915
NLE100594622
MCC715747(CYP27B1)
MCF102117707(CYP27B1)
CSAB103238591(CYP27B1)
CATY105587234
PANU101009903
TGE112634833
RRO104682342
RBB108524407
TFN117089857
PTEH111541786
CJC100388588(CYP27B1)
SBQ101042462
CSYR103264442
MMUR105877998
OGA100961635
MMU13115(Cyp27b1)
MCAL110303958
MPAH110327080
RNO114700(Cyp27b1)
MCOC116075946
MUN110561784
CGE100760414
PLEU114704779
NGI103742896
HGL101700851
CPOC100717678
CCAN109686086
DORD105990140
DSP122124179
NCAR124983979
OCU100341053
OPI101530921
TUP102492875
CFA481133(CYP27B1)
VVP112922328
VLG121491053
AML100481484
UMR103656705
UAH113256358
UAR123787194
ELK111160181
LLV125106498
MPUF101674682
ORO101363421
EJU114215050
ZCA113922553
MLX118008655
FCA100750221(CYP27B1)
PYU121040851
PBG122473840
PTG102971063
PPAD109270394
AJU106975877
HHV120248015
BTA539630(CYP27B1)
BOM102278257
BIU109559305
BBUB102414554
CHX102170758
OAS101116039
ODA120857512
CCAD122427212
SSC397055(CYP27B1)
CFR102522811
CBAI105062143
CDK105100328
VPC102532825
BACU103001013
LVE103084276
OOR101284262
DLE111173817
PCAD102984886
PSIU116760774
ECB100034100(CYP27B1)
EPZ103553643
EAI106833020
MYB102262846
MYD102757775
MMYO118649575
MLF102431071
MNA107546167
PKL118730267
HAI109390756
DRO112319168
SHON118975885
AJM119065237
PDIC114513469
PHAS123812846
MMF118623548
RFQ117028656
PALE102894468
PGIG120604970
PVP105307396
RAY107501490
MJV108387803
TOD119248104
SARA101536895
LAV100657886
TMU101355020
DNM101414278
MDO100031497
GAS123250198
SHR100913970
PCW110210991
OAA114814717
LSR110473893
SCAN103824978
PMOA120499536
OTC121334301
PRUF121356351
FAB101816678
PHI102103763
PMAJ107199279
NNI104021543
TALA116963700 122153819
ACHC115350948
AROW112972148
NPD112952263
ASN102386966
AMJ102574984
CPOO109324487
PSS102443605
CMY102946801
CPIC101954100
TST117888845
CABI116828443
MRV120391239
ACS100559713
PVT110074451
SUND121920838
PBI103060795
PMUR107291644
TSR106552999
PGUT117662682
VKO123029856
PMUA114593032
ZVI118078986
GJA107119675
XLA108708601(cyp27b1.L) 108709871(cyp27b1.S)
XTR448754(cyp27b1)
NPR108791588
RTEM120929439
BBUF120996179
BGAR122931986
DRE793309(cyp27b1)
SRX107747831
SANH107684010
SGH107561793
CCAR109089182
CAUA113068164
IPU108254794
SMEO124402328
TFD113639045
AMEX103035224
EEE113572450
TRU101062641
TNGGSTEN00033821G001
LCO109142364
NCC104955571
CGOB115008521
ELY117257290
PLEP121956007
SLUC116066246
ECRA117942881
PFLV114554512
GAT120835315
PPUG119194159
MSAM119886596
CUD121528681
MZE101476393
ONL100706100
OAU116326616
OLA101172325
OML112145670
XMA102222483
XCO114134887
XHE116709734
PRET103465325
PFOR103129584
PLAI106935833
PMEI106929457
GAF122834207
CVG107082096
CTUL119795482
GMU124856373
NFU107385653
KMR108235620
ALIM106518084
NWH119412594
AOCE111587639
CSEM103386049
POV109628943
SSEN122767755
HHIP117761420
LCF108898968
SDU111221905
SLAL111667688
XGL120806983
HCQ109522134
BPEC110171326
MALB109956137
SASA106565446 106583283
OTW112254699 112262073
OMY110494198 110528221
OGO124003988 124019410
ONE115143688 115146075
SALP111965588 111970194
SNH120062013 120064579
ELS105017033
SFM108918367 108939170
PKI111854850
AANG118210837
LOC102685924
PSPA121308962
ARUT117400985 117966943
LCM102346327
CMK103191415
RTP109925523
PPOT106099942
BGT106053581
GAE121389521
CRG105346613
MMER123550682
OSN115209661
ATEN116299173 116299174
 » show all
Reference
1  [PMID:4404596]
  Authors
Gray RW, Omdahl JL, Ghazarian JG, DeLuca HF.
  Title
25-Hydroxycholecalciferol-1-hydroxylase. Subcellular location and properties.
  Journal
J Biol Chem 247:7528-32 (1972)
Reference
2  [PMID:10092858]
  Authors
Sakaki T, Sawada N, Takeyama K, Kato S, Inouye K
  Title
Enzymatic properties of mouse 25-hydroxyvitamin D3 1 alpha-hydroxylase expressed  in Escherichia coli.
  Journal
Eur J Biochem 259:731-8 (1999)
DOI:10.1046/j.1432-1327.1999.00096.x
Reference
3  [PMID:10518789]
  Authors
Sawada N, Sakaki T, Kitanaka S, Takeyama K, Kato S, Inouye K
  Title
Enzymatic properties of human 25-hydroxyvitamin D3 1alpha-hydroxylase coexpression with adrenodoxin and NADPH-adrenodoxin reductase in Escherichia coli.
  Journal
Eur J Biochem 265:950-6 (1999)
DOI:10.1046/j.1432-1327.1999.00794.x
Other DBs
ExplorEnz - The Enzyme Database: 1.14.15.18
IUBMB Enzyme Nomenclature: 1.14.15.18
ExPASy - ENZYME nomenclature database: 1.14.15.18
BRENDA, the Enzyme Database: 1.14.15.18
CAS: 9081-36-1

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