Entry
Name
calcidiol 1-monooxygenase;
25-hydroxycholecalciferol 1-hydroxylase;
25-hydroxycholecalciferol 1-monooxygenase;
1-hydroxylase-25-hydroxyvitamin D3;
25-hydroxy D3-1alpha-hydroxylase;
25-hydroxycholecalciferol 1alpha-hydroxylase;
25-hydroxyvitamin D3 1alpha-hydroxylase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
calcidiol,adrenodoxin:oxygen oxidoreductase (1-hydroxylating)
Reaction(IUBMB)
(1) calcidiol + 2 reduced adrenodoxin + 2 H+ + O2 = calcitriol + 2 oxidized adrenodoxin + H2O [RN:
R03610 ];
(2) secalciferol + 2 reduced adrenodoxin + 2 H+ + O2 = calcitetrol + 2 oxidized adrenodoxin + H2O [RN:
R11324 ]
Reaction(KEGG)
Substrate
Product
Comment
A P-450 (heme-thiolate) enzyme found in mammals.
History
EC 1.14.15.18 created 1976 as EC 1.14.13.13, transferred 2016 to EC 1.14.15.18
Pathway
Orthology
K07438 calcidiol 1-monooxygenase (25-hydroxyvitamin D3 1alpha-hydroxylase)
Genes
TALA : 116963700 122153819
XLA : 108708601(cyp27b1.L) 108709871(cyp27b1.S)
MAMB : 125256888 125261911
SASA : 106565446 106583283
STRU : 115150468 115207954
SALP : 111965588 111970194
CCLU : 121575162 121577839
ARUT : 117400985 117966943
PVUL : 126815583 126817612 126819097
ATEN : 116299173 116299174
» show all
Taxonomy
Reference
Authors
Gray RW, Omdahl JL, Ghazarian JG, DeLuca HF.
Title
25-Hydroxycholecalciferol-1-hydroxylase. Subcellular location and properties.
Journal
J Biol Chem 247:7528-32 (1972)
Reference
Authors
Sakaki T, Sawada N, Takeyama K, Kato S, Inouye K
Title
Enzymatic properties of mouse 25-hydroxyvitamin D3 1 alpha-hydroxylase expressed in Escherichia coli.
Journal
Reference
Authors
Sawada N, Sakaki T, Kitanaka S, Takeyama K, Kato S, Inouye K
Title
Enzymatic properties of human 25-hydroxyvitamin D3 1alpha-hydroxylase coexpression with adrenodoxin and NADPH-adrenodoxin reductase in Escherichia coli.
Journal
Other DBs