KEGG   ENZYME: 1.14.15.22Help
Entry
EC 1.14.15.22               Enzyme                                 

Name
vitamin D 1,25-hydroxylase;
CYP105A1;
Streptomyces griseolus cytochrome P450SU-1
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
calciol,ferredoxin:oxygen oxidoreductase (1,25-hydroxylating)
Reaction(IUBMB)
(1) calciol + O2 + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ = calcidiol + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O [RN:R11458];
(2) calcidiol + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 = calcitriol + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O [RN:R11459]
Reaction(KEGG)
Substrate
calciol [CPD:C05443];
O2 [CPD:C00007];
reduced ferredoxin [iron-sulfur] cluster [CPD:C00138];
H+ [CPD:C00080];
calcidiol [CPD:C01561]
Product
calcidiol [CPD:C01561];
oxidized ferredoxin [iron-sulfur] cluster [CPD:C00139];
H2O [CPD:C00001];
calcitriol [CPD:C01673]
Comment
A P-450 (heme-thiolate) enzyme found in the bacterium Streptomyces griseolus. cf. EC 1.14.14.24, vitamin D 25-hydroxylase and EC 1.14.15.18, calcidiol 1-monooxygenase.
History
EC 1.14.15.22 created 2016
Pathway
ec00100  Steroid biosynthesis
ec01100  Metabolic pathways
Orthology
K21146  vitamin D 1,25-hydroxylase
Genes
NBR: O3I_016665
SSX: SACTE_6300
SALU: DC74_467
SALL: SAZ_02815
STRE: GZL_08768
SNR: SNOUR_39820
Taxonomy
Reference
1  [PMID:15207715]
  Authors
Sawada N, Sakaki T, Yoneda S, Kusudo T, Shinkyo R, Ohta M, Inouye K
  Title
Conversion of vitamin D3 to 1alpha,25-dihydroxyvitamin D3 by Streptomyces griseolus cytochrome P450SU-1.
  Journal
Biochem Biophys Res Commun 320:156-64 (2004)
DOI:10.1016/j.bbrc.2004.05.140
  Sequence
Reference
2  [PMID:18314962]
  Authors
Sugimoto H, Shinkyo R, Hayashi K, Yoneda S, Yamada M, Kamakura M, Ikushiro S, Shiro Y, Sakaki T
  Title
Crystal structure of CYP105A1 (P450SU-1) in complex with 1alpha,25-dihydroxyvitamin D3.
  Journal
Biochemistry 47:4017-27 (2008)
DOI:10.1021/bi7023767
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.15.22
IUBMB Enzyme Nomenclature: 1.14.15.22
ExPASy - ENZYME nomenclature database: 1.14.15.22
BRENDA, the Enzyme Database: 1.14.15.22

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