KEGG   ENZYME: 1.14.15.31
Entry
EC 1.14.15.31               Enzyme                                 
Name
2-hydroxy-5-methyl-1-naphthoate 7-hydroxylase;
NcsB3
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
2-hydroxy-5-methyl-1-naphthoate,reduced ferredoxin:oxygen oxidoreductase (7-hydroxylating)
Reaction(IUBMB)
2-hydroxy-5-methyl-1-naphthoate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 = 2,7-dihydroxy-5-methyl-1-naphthoate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O [RN:R10796]
Reaction(KEGG)
R10796
Substrate
2-hydroxy-5-methyl-1-naphthoate [CPD:C20856];
reduced ferredoxin [iron-sulfur] cluster [CPD:C00138];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
2,7-dihydroxy-5-methyl-1-naphthoate [CPD:C20857];
oxidized ferredoxin [iron-sulfur] cluster [CPD:C00139];
H2O [CPD:C00001]
Comment
A cytochrome P-450 (heme-thiolate) protein involved in the synthesis of neocarzinostatin in the bacterium Streptomyces carzinostaticus.
History
EC 1.14.15.31 created 2014 as EC 1.14.99.49, transferred 2018 to EC 1.14.15.31
Pathway
ec01059  Biosynthesis of enediyne antibiotics
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K20420  2-hydroxy-5-methyl-1-naphthoate 7-hydroxylase
Genes
LBQCKQ53_05320
ETPLU633_02190
XORXOC_0085
XOZBE73_23900
XTNFD63_13545
XTHG4Q83_03485
MLOmll6125
MERDEB233_29925
NFANFA_22930 NFA_45180 NFA_53110
NFRERS450000_01804 ERS450000_04570 ERS450000_05413
NCYNOCYR_0216 NOCYR_3413 NOCYR_4397 NOCYR_4398
NBRO3I_001245 O3I_016330 O3I_021950 O3I_027950
NNONONO_c13610 NONO_c15520 NONO_c55450
NSLBOX37_14605 BOX37_23285
NSRNS506_02937 NS506_04941
NTPCRH09_09685 CRH09_15665 CRH09_18305
NOZDMB37_06955 DMB37_22310 DMB37_37765
NODFOH10_08035 FOH10_19640 FOH10_22545
NAHF5544_08475 F5544_18890
NADNCTC11293_03171 NCTC11293_04204 NCTC11293_05655
NWLNWFMUON74_26820 NWFMUON74_41780 NWFMUON74_44890 NWFMUON74_51250 NWFMUON74_53680
NIEKV110_01240 KV110_15580 KV110_16390 KV110_18315 KV110_27215
NTCKHQ06_14085 KHQ06_15520 KHQ06_27165
NHUH0264_13770 H0264_14795
NYALTV02_08580 LTV02_15325 LTV02_18310 LTV02_19085
NGPLTT66_00215 LTT66_00410 LTT66_00425 LTT66_07700 LTT66_28845
SCOSCO2884(SCE6.21) SCO7417(SC6D11.13c)
SALBXNR_0810 XNR_2289 XNR_2319
SMASAVERM_109(cyp1) SAVERM_1308(cyp6) SAVERM_3704(cyp17) SAVERM_3882(cyp19)
SGRSGR_1085 SGR_3108 SGR_622
SGBWQO_20200 WQO_30190
SCBSCAB_20211 SCAB_32131
SSXSACTE_2263 SACTE_3804 SACTE_6025
SVLStrvi_2196 Strvi_7659
SHYSHJG_0095 SHJG_2299 SHJG_8229
SHOSHJGH_2064 SHJGH_7990
SRCM271_14635 M271_32985
SMALSMALA_2464 SMALA_5783
SSOII1A49_15785 I1A49_32635
SFASfla_0779 Sfla_2701 Sfla_4117 Sfla_5505
SBHSBI_01881(cyp20) SBI_07324(cyp35) SBI_07588(cyp38)
SVESVEN_0422
SDVBN159_0797 BN159_1304
SALSSLNWT_4502 SLNWT_7122
STRPF750_1106 F750_2599 F750_4099 F750_6081
SFISFUL_4231 SFUL_6410
SCIB446_03455
SALUDC74_1310 DC74_3059 DC74_329
SALLSAZ_02095 SAZ_07045 SAZ_16330
SLVSLIV_00215 SLIV_02225 SLIV_23265
SGUSGLAU_25545(cyp17) SGLAU_31595
SVTSVTN_04005 SVTN_35390
STREGZL_05877 GZL_08215
SCWTU94_00970 TU94_03385
SLDT261_05751
SLCSL103_06415
SXISXIM_32420
STRMM444_02645 M444_05420 M444_06720 M444_37900
STRCAA958_20030
SAMBSAM23877_0230(cyp107B) SAM23877_0787 SAM23877_0848 SAM23877_7443(cyp107B)
SPRISPRI_0135 SPRI_0320 SPRI_4276 SPRI_7033 SPRI_7218
SCZABE83_03465 ABE83_04305 ABE83_15155
SCXAS200_25700 AS200_41585
SRWTUE45_00479 TUE45_00829(eryF) TUE45_01059(vdh_1)
STRFASR50_18825
SLEsle_03480(sle_03480) sle_12110(sle_12110) sle_62170(sle_62170)
SPAVSpa2297_01655 Spa2297_12320
STRTA8713_01270 A8713_29885
SCLFBB341_11715 BB341_27285 BB341_27625 BB341_27875
SGSAVL59_26255 AVL59_26840 AVL59_28135 AVL59_32365
STSIA4E84_01955 A4E84_20615
SKYD0C37_00925 D0C37_05465 D0C37_13220 D0C37_13385
SALWCP975_15440 CP975_30160
SGMGCM10017557_33360 GCM10017557_56600
SNRSNOUR_00270 SNOUR_06910 SNOUR_27655 SNOUR_36945(cyp6) SNOUR_43680
SPLULK06_000705 LK06_002680
STRDNI25_24330
SNWBBN63_00305 BBN63_09460 BBN63_21345
SAUOBV401_15850 BV401_32800
SSIAA7J05_00560 A7J05_19955 A7J05_33655 A7J05_36175
SVUB1H20_02120 B1H20_02150 B1H20_20375 B1H20_31005
SPUNBFF78_07355
SGVB1H19_14615
SLAUSLA_0018 SLA_0318 SLA_6823
SALJSMD11_0702 SMD11_1711
SLXSLAV_06825(vdh1) SLAV_33495
STROSTRMOE7_13470
SFKKY5_0136c KY5_0744 KY5_3596 KY5_6077 KY5_8070c
SNZDC008_00615 DC008_32125
SRJSRO_1158 SRO_1505
SLKSLUN_00485 SLUN_15750 SLUN_38635
SGDELQ87_01940 ELQ87_05105 ELQ87_36190
SQZFQU76_13310 FQU76_22270 FQU76_26530 FQU76_32330
SCYAEJ357_35275 EJ357_42475 EJ357_45190
STIRDDW44_00810 DDW44_29175 DDW44_31375
SKACP970_10575 CP970_24295 CP970_39280 CP970_43350
SGZC0216_11955
SVNCP980_02070 CP980_05510
SNKCP967_02920 CP967_04280 CP967_14380 CP967_15145 CP967_22285
SHAWCEB94_02430 CEB94_20755
SRKFGW37_24875
SFICEIZ62_05535 EIZ62_15365 EIZ62_18440 EIZ62_30720
SGALCP966_34265
SSPODDQ41_00860 DDQ41_21455 DDQ41_30005 DDQ41_30940
SVRCP971_10550 CP971_15130
SPADDVK44_27765
SFYGFH48_22780 GFH48_36815 GFH48_38935
SAQUEJC51_22545 EJC51_36380
SGFHEP81_01125 HEP81_01693 HEP81_07958
SCAVCVT27_19390 CVT27_29455 CVT27_30230
SSEOD0Z67_03615 D0Z67_22675 D0Z67_28045
SBYH7H31_06885 H7H31_25020
SRIMCP984_15090 CP984_35310 CP984_38550
SSUBCP968_03305 CP968_05965 CP968_30745
SPHVF9278_38030
SCINCP977_01840 CP977_03670 CP977_10085 CP977_31880
SFUGCNQ36_15300
SPACB1H29_23075
STSUB7R87_00250 B7R87_01695 B7R87_12915 B7R87_21820 B7R87_26385
SCHACP983_37555 CP983_41400
SGXH4W23_04555 H4W23_36190 H4W23_37305
SCOECP976_22205 CP976_33635 CP976_40710
SBATG4Z16_02345 G4Z16_08235 G4Z16_08740 G4Z16_19925
SSPBCP982_02885 CP982_39980
SNFJYK04_06995(ncsB3)
SPLACP981_12975
SBROGQF42_06550 GQF42_39935
STUIGCM10017668_35600 GCM10017668_55660 GCM10017668_67040
SATAC5746_23480
SCHFIPT68_16280
SHUNDWB77_04303(ncsB3)
SHARHUT13_16700
SCYGS1361_02770(eryF1)
SFEUIM697_24500 IM697_25075
SLIAHA039_18790
SDWK7C20_12250 K7C20_23035
SVDCP969_00465 CP969_17840 CP969_20590
SCALI6J39_02245 I6J39_02275 I6J39_20185 I6J39_30810
SAUHSU9_010515 SU9_021050 SU9_030625
SMOBJ7W19_13355 J7W19_24670
SPRACP972_03595 CP972_16040 CP972_28990
SPEUCGZ69_34815
SROIIAG44_02330 IAG44_03650 IAG44_38110
SXNIAG42_07270 IAG42_15655 IAG42_31110 IAG42_33875
SGJIAG43_00205 IAG43_00935 IAG43_13315 IAG43_31565
SHKJ2N69_03375 J2N69_15875
SANUK7396_04935 K7396_13960 K7396_24345
STRYEQG64_29730 EQG64_30775 EQG64_31580
SDDD9753_02285 D9753_32560
SACTDMT42_02400
SMAOCAG99_15930 CAG99_18330
SGOBtest1122_06935
SINEKI385_15970
SLFJEQ17_15890 JEQ17_19930 JEQ17_43355 JEQ17_49110
SDECL3078_35525 L3078_43210
SAOVG3H79_00240 G3H79_02610 G3H79_38355 G3H79_40300
KSKKSE_03080 KSE_07770 KSE_19190 KSE_19210 KSE_42760 KSE_74300
KABB7C62_00645 B7C62_20460 B7C62_32065
KAUB6264_05620 B6264_10165
KITCFP65_4254 CFP65_5244 CFP65_5759 CFP65_7617
STRIC7M71_026660
YIALO772_22550
TFUTfu_1257 Tfu_1748 Tfu_2802 Tfu_2976
THAONI17_007995 NI17_008210 NI17_017550 NI17_023955
TALXFOF52_03235 FOF52_08235 FOF52_14995 FOF52_16170
NDANdas_2750 Ndas_4555
NALB005_0905 B005_1483 B005_2426 B005_5303
NEXNE857_03680 NE857_19420 NE857_19430 NE857_21025
STRREKD16_06410 EKD16_16105 EKD16_18695(vdh1) EKD16_18950(vdh2) EKD16_21840(vdh3) EKD16_23225
TCUTcur_1432
AGRAAGRA3207_001012
SROSros_6983 Sros_8715
NOABKM31_06905 BKM31_10805 BKM31_24015 BKM31_30205 BKM31_50570
NOWGBF35_14365 GBF35_31095 GBF35_34610
NCXNocox_13485 Nocox_24380
NGNLCN96_23305 LCN96_30555 LCN96_32525 LCN96_34585 LCN96_50775
FALFRAAL1514
AMDAMED_7339
AMNRAM_37725
AMMAMES_7228
AMZB737_7228
AOIAORI_4218
AJAAJAP_17820
AMYCCU254_13715 CU254_41355
AMYBBKN51_28845 BKN51_28940 BKN51_41810
AABA4R43_37295 A4R43_40390
AMYYYIM_37285 YIM_40790
AORISD37_05155 SD37_23115
APRECNX65_15545
AMIAmir_3059
SESPBN6_38980 BN6_40190 BN6_48460
SSYIEKG83_16920 EKG83_22955 EKG83_30320
KALKALB_3732
KPHYAOZ06_23035 AOZ06_31150
AHMTL08_17105 TL08_17125
ACTIUA75_16455 UA75_20810 UA75_20825
ACADUA74_15990 UA74_20305 UA74_20320
AHGAHOG_16510(eryF) AHOG_18165 AHOG_18180
ACTAC1701_20910
ALOCRK59466
ACTUActkin_05259
STPStrop_2724
SAQSare_4949
MTEMGCE86_08090
VMAVAB18032_17045 VAB18032_29041
AIHAiant_18010
PLABC6361_26025 C6361_26075
PLATC6W10_12775 C6W10_12825
PSUUPsuf_070200
CAICaci_4185 Caci_5282
AEYCDG81_11605 CDG81_12515
 » show all
Reference
1  [PMID:20735485]
  Authors
Hang VT, Oh TJ, Yamaguchi T, Sohng JK
  Title
In vivo characterization of NcsB3 to establish the complete biosynthesis of the naphthoic acid moiety of the neocarzinostatin chromophore.
  Journal
FEMS Microbiol Lett 311:119-25 (2010)
DOI:10.1111/j.1574-6968.2010.02075.x
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.15.31
IUBMB Enzyme Nomenclature: 1.14.15.31
ExPASy - ENZYME nomenclature database: 1.14.15.31
BRENDA, the Enzyme Database: 1.14.15.31

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