KEGG   ENZYME: 1.14.15.38
Entry
EC 1.14.15.38               Enzyme                                 

Name
N,N-dimethyl phenylurea N-demethylase;
pdmAB (gene names)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
N,N-dimethyl-N'-phenylurea compound,NAD(P)H:oxygen oxidoreductase (formaldehyde-forming)
Reaction(IUBMB)
an N,N-dimethyl-N'-phenylurea compound + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 = an N-methyl-N'-phenylurea compound + formaldehyde + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O [RN:R12673]
Reaction(KEGG)
R12673
Substrate
N,N-dimethyl-N'-phenylurea compound [CPD:C22321];
reduced ferredoxin [iron-sulfur] cluster [CPD:C00138];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
N-methyl-N'-phenylurea compound [CPD:C22322];
formaldehyde [CPD:C00067];
oxidized ferredoxin [iron-sulfur] cluster [CPD:C00139];
H2O [CPD:C00001]
Comment
The enzyme, found in members of the Sphingobium genus, initiates the degradation of N,N-dimethyl-phenylurea herbicides by mono-N-demethylation. The catalytic unit contains a Rieske [2Fe-2S] iron-sulfur cluster, and catalyses the monooxygenation of a methyl group. The resulting N-methoxyl group is unstable and decomposes spontaneously to form formaldehyde. The enzyme associates with additional proteins (a reductase and a [3Fe-4S] type ferredoxin) that are involved in the transfer of electrons from NAD(P)H to the active site.
History
EC 1.14.15.38 created 2020
Orthology
K24702  N,N-dimethyl phenylurea N-demethylase alpha subunit
K24703  N,N-dimethyl phenylurea N-demethylase beta subunit
Genes
ABOU: ACBO_15530 ACBO_15540
SYB: TZ53_25065 TZ53_25070
SPHT: K426_22024 K426_22029
SHYD: CJD35_19475 CJD35_19480
MSA: Mycsm_01928 Mycsm_01929
Reference
1  [PMID:24123738]
  Authors
Gu T, Zhou C, Sorensen SR, Zhang J, He J, Yu P, Yan X, Li S.
  Title
The novel bacterial N-demethylase PdmAB is responsible for the initial step of N,N-dimethyl-substituted phenylurea herbicide degradation.
  Journal
Appl Environ Microbiol 79:7846-56 (2013)
DOI:10.1128/AEM.02478-13
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.15.38
IUBMB Enzyme Nomenclature: 1.14.15.38
ExPASy - ENZYME nomenclature database: 1.14.15.38
BRENDA, the Enzyme Database: 1.14.15.38

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