Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen into the other donor
progesterone,reduced-ferredoxin:oxygen oxidoreductase (15beta-hydroxylating)
progesterone + 2 reduced [2Fe-2S] ferredoxin + O2 = 15beta-hydroxyprogesterone + 2 oxidized [2Fe-2S] ferredoxin + H2O [RN:
oxidized [2Fe-2S] ferredoxin [CPD:
The enzyme from the bacterium Bacillus megaterium hydroxylates a variety of 3-oxo-Delta4-steroids in position 15beta. Ring A-reduced, aromatic, and 3beta-hydroxy-Delta4-steroids do not serve as substrates .
EC 220.127.116.11 created 2010
K21114 steroid 15beta-monooxygenase
Berg A, Ingelman-Sundberg M, Gustafsson JA
Purification and characterization of cytochrome P-450meg.
J Biol Chem 254:5264-71 (1979)
Berg A, Gustafsson JA, Ingelman-Sundberg M
Characterization of a cytochrome P-450-dependent steroid hydroxylase system present in Bacillus megaterium.
J Biol Chem 251:2831-8 (1976)
Lisurek M, Kang MJ, Hartmann RW, Bernhardt R
Identification of monohydroxy progesterones produced by CYP106A2 using comparative HPLC and electrospray ionisation collision-induced dissociation mass spectrometry.
Goni G, Zollner A, Lisurek M, Velazquez-Campoy A, Pinto S, Gomez-Moreno C, Hannemann F, Bernhardt R, Medina M
Cyanobacterial electron carrier proteins as electron donors to CYP106A2 from Bacillus megaterium ATCC 13368.
Lisurek M, Simgen B, Antes I, Bernhardt R
Theoretical and experimental evaluation of a CYP106A2 low homology model and production of mutants with changed activity and selectivity of hydroxylation.
ExPASy - ENZYME nomenclature database: 18.104.22.168