KEGG   ENZYME: 1.14.18.12
Entry
EC 1.14.18.12               Enzyme                                 

Name
2-hydroxy fatty acid dioxygenase;
MPO1 (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With another compound as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
2-hydroxy fatty acid:oxygen oxidoreductase (CO2,H2O-forming)
Reaction(IUBMB)
a (2R)-2-hydroxy Cn-fatty acid + O2 = a Cn-1-fatty acid + H2O + CO2 [RN:R12585]
Reaction(KEGG)
R12585
Substrate
(2R)-2-hydroxy Cn-fatty acid;
O2 [CPD:C00007]
Product
Cn-1-fatty acid;
H2O [CPD:C00001];
CO2 [CPD:C00011]
Comment
Requires Fe(II). The enzyme, characterized from yeast, is involved in phytosphingosine metabolism. The reaction is mediated by iron(IV) peroxide and results in the release of a water molecule and a carbon dioxide molecule, shortening the substrate by a single carbon atom and forming an odd-numbered fatty acid. Both oxygen atoms of the original carboxylate group are released - one as the leaving water molecule, the other as one of the oxygens of the carbon dioxide molecule. The two oxygen atoms in the newly-formed carboxylate originate from the 2-hydroxy group and from molecular oxygen, respectively. The other oxygen atom of the molecular oxygen is incorporated into the leaving CO2 molecule. The enzyme from the yeast Saccharomyces cerevisiae is active at least toward C14 to C26 2-hydroxy fatty acids, but not against C8 2-hydroxy fatty acid.
History
EC 1.14.18.12 created 2020
Orthology
K24121  2-hydroxy fatty acid dioxygenase
Genes
SCE: YGL010W(MPO1)
AGO: AGOS_AGR171C
ERC: Ecym_7232
KLA: KLLA0_A05005g
KMX: KLMA_50156
LTH: KLTH0E07766g
VPO: Kpol_1061p46
ZRO: ZYRO0G10120g
CGR: CAGL0A00341g
NCS: NCAS_0A01650(NCAS0A01650)
NDI: NDAI_0D03570(NDAI0D03570)
TPF: TPHA_0F00660(TPHA0F00660)
TBL: TBLA_0C04090(TBLA0C04090)
TDL: TDEL_0F02370(TDEL0F02370)
KAF: KAFR_0C02100(KAFR0C02100)
CAL: CAALFM_C201660CA(CaO19.1477)
NCR: NCU02660
NTE: NEUTE1DRAFT91884(NEUTE1DRAFT_91884)
MGR: MGG_00509
SSCK: SPSK_09755
MAW: MAC_04150
MAJ: MAA_07224
CMT: CCM_07062
BFU: BCIN_15g01810(Bcmpo1)
MBE: MBM_02330
ANI: AN1522.2
ANG: ANI_1_986144(An16g07420)
ABE: ARB_06552
TVE: TRV_04430
CNE: CNC04620
CNB: CNBC2550
TASA: A1Q1_03768
ABP: AGABI1DRAFT113620(AGABI1DRAFT_113620)
ABV: AGABI2DRAFT191730(AGABI2DRAFT_191730)
MGL: MGL_2025
MRT: MRET_1556
 » show all
Reference
1  [PMID:25345524]
  Authors
Kondo N, Ohno Y, Yamagata M, Obara T, Seki N, Kitamura T, Naganuma T, Kihara A
  Title
Identification of the phytosphingosine metabolic pathway leading to odd-numbered  fatty acids.
  Journal
Nat Commun 5:5338 (2014)
DOI:10.1038/ncomms6338
  Sequence
[sce:YGL010W]
Reference
2  [PMID:30530523]
  Authors
Seki N, Mori K, Kitamura T, Miyamoto M, Kihara A
  Title
Yeast Mpo1 Is a Novel Dioxygenase That Catalyzes the alpha-Oxidation of a 2-Hydroxy Fatty Acid in an Fe(2+)-Dependent Manner.
  Journal
Mol Cell Biol 39 (2019)
DOI:10.1128/MCB.00428-18
Other DBs
ExplorEnz - The Enzyme Database: 1.14.18.12
IUBMB Enzyme Nomenclature: 1.14.18.12
ExPASy - ENZYME nomenclature database: 1.14.18.12
BRENDA, the Enzyme Database: 1.14.18.12

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