Entry
Name
CMP-N-acetylneuraminate monooxygenase;
CMP-N-acetylneuraminic acid hydroxylase;
CMP-Neu5Ac hydroxylase;
cytidine monophosphoacetylneuraminate monooxygenase;
N-acetylneuraminic monooxygenase;
cytidine-5'-monophosphate-N-acetylneuraminic acid hydroxylase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With another compound as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
CMP-N-acetylneuraminate,ferrocytochrome-b5:oxygen oxidoreductase (N-acetyl-hydroxylating)
Reaction(IUBMB)
CMP-N-acetylneuraminate + 2 ferrocytochrome b5 + O2 + 2 H+ = CMP-N-glycoloylneuraminate + 2 ferricytochrome b5 + H2O [RN:
R01115 ]
Reaction(KEGG)
Substrate
Product
CMP-N-glycoloylneuraminate [CPD:
C03691 ];
ferricytochrome b5 [CPD:
C00996 ];
H2O [CPD:
C00001 ]
Comment
This enzyme contains both a Rieske-type [2Fe-2S] cluster and a second iron site. The ferricytochrome b5 produced is reduced by NADH and cytochrome-b5 reductase (EC
1.6.2.2 ). The enzyme can be activated by Fe2+ or Fe3+.
History
EC 1.14.18.2 created 1992 as EC 1.14.13.45, transferred 2003 to EC 1.14.18.2
Pathway
ec00520 Amino sugar and nucleotide sugar metabolism
Orthology
K08080 CMP-N-acetylneuraminate monooxygenase
Genes
RTEM : 120940782 120940783
BBUF : 121002189 121002190
BGAR : 122931557 122938794 122939448
SANH : 107656456 107658750
CCAR : 109063653 109102374
CAUA : 113048495 113059734
CGIB : 127956500 127979083
LROH : 127163864 127163865
CIDE : 127511545 127511546
MASI : 127435451 127436525
TROS : 130547845(cmah) 130550021
MANU : 129432719 129432720(cmah)
SPIL : 134061667 134061668 134061801 134061802 134062096
TRU : 115246491 115246687 777967
GACU : 117551232 117551235
MSAM : 119918133 119918134 119918437
LMIX : 132956293(cmah) 132956309
SASA : 106604887 106609768 106609788
STRU : 115180355 115199149
OTW : 112214917(cmah) 121845873
OMY : 110499771 110499779 110499782 110500969 110500972 110518324
OKI : 109876981 109876982 116363549
AANG : 118220432 118221204 118221926 118221927
BFO : 118410108 118418888 118420159 118424237 118430247
BBEL : 109465764 109470655 109473073 109477656 109487371
LPIC : 129273945 129274958
APLC : 110979354 110986734
PFLL : 139115580 139116902 139134685 139134859 139138234
HFE : HFELIS_01840 HFELIS_09990
» show all
Taxonomy
Reference
Authors
Shaw L, Schauer R.
Title
The biosynthesis of N-glycoloylneuraminic acid occurs by hydroxylation of the CMP-glycoside of N-acetylneuraminic acid.
Journal
Reference
Authors
Kozutsumi Y, Kawano T, Yamakawa T, Suzuki A.
Title
Participation of cytochrome b5 in CMP-N-acetylneuraminic acid hydroxylation in mouse liver cytosol.
Journal
Reference
Authors
Schneckenburger P, Shaw L, Schauer R.
Title
Purification, characterization and reconstitution of CMP-N-acetylneuraminate hydroxylase from mouse liver.
Journal
Reference
Authors
Kawano T, Koyama S, Takematsu H, Kozutsumi Y, Kawasaki H, Kawashima S, Kawasaki T, Suzuki A
Title
Molecular cloning of cytidine monophospho-N-acetylneuraminic acid hydroxylase. Regulation of species- and tissue-specific expression of N-glycolylneuraminic acid.
Journal
Sequence
Reference
Authors
Schlenzka W, Shaw L, Kelm S, Schmidt CL, Bill E, Trautwein AX, Lottspeich F, Schauer R.
Title
CMP-N-acetylneuraminic acid hydroxylase: the first cytosolic Rieske iron-sulphur protein to be described in Eukarya.
Journal
Sequence
Other DBs
ExPASy - ENZYME nomenclature database: 1.14.18.2