ricinoleic acid synthase;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With another compound as one donor, and incorporation of one atom of oxygen into the other donor
1-acyl-2-oleoyl-sn-glycero-3-phosphocholine,ferrocytochrome-b5:oxygen oxidoreductase (12-hydroxylating)
a 1-acyl-2-oleoyl-sn-glycero-3-phosphocholine + 2 ferrocytochrome b5 + O2 + 2 H+ = a 1-acyl-2-[(12R)-12-hydroxyoleoyl]-sn-glycero-3-phosphocholine + 2 ferricytochrome b5 + H2O [RN:
ferrocytochrome b5 [CPD:
ferricytochrome b5 [CPD:
The enzyme, characterized from the plant Ricinus communis (castor bean), is involved in production of the 12-hydroxylated fatty acid ricinoleate. The enzyme, which shares sequence similarity with fatty-acyl desaturases, requires a cytochrome b5 as the electron donor.
EC 188.8.131.52 created 1984 as EC 184.108.40.206, transferred 2015 to EC 220.127.116.11
K21704 phosphatidylcholine 12-monooxygenase
Galliard T, Stumpf PK.
Fat metabolism in higher plants. 30. Enzymatic synthesis of ricinoleic acid by a microsomal preparation from developing Ricinus communis seeds.
J Biol Chem 241:5806-12 (1966)
Moreau RA, Stumpf PK.
Recent Studies of the Enzymic Synthesis of Ricinoleic Acid by Developing Castor Beans.
Smith MA, Jonsson L, Stymne S, Stobart K
Evidence for cytochrome b5 as an electron donor in ricinoleic acid biosynthesis in microsomal preparations from developing castor bean (Ricinus communis L.).
Lin JT, McKeon TA, Goodrich-Tanrikulu M, Stafford AE
Characterization of oleoyl-12-hydroxylase in castor microsomes using the putative substrate, 1-acyl-2-oleoyl-sn-glycero-3-phosphocholine.
Broun P, Somerville C
Accumulation of ricinoleic, lesquerolic, and densipolic acids in seeds of transgenic Arabidopsis plants that express a fatty acyl hydroxylase cDNA from castor bean.
ExPASy - ENZYME nomenclature database: 18.104.22.168