KEGG   ENZYME: 1.14.18.8Help
Entry
EC 1.14.18.8                Enzyme                                 

Name
7alpha-hydroxycholest-4-en-3-one 12alpha-hydroxylase;
7alpha-hydroxy-4-cholesten-3-one 12alpha-monooxygenase;
CYP12;
sterol 12alpha-hydroxylase (ambiguous);
HCO 12alpha-hydroxylase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With another compound as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
7alpha-hydroxycholest-4-en-3-one,ferrocytochrome-b5:oxygen oxidoreductase (12alpha-hydroxylating)
Reaction(IUBMB)
7alpha-hydroxycholest-4-en-3-one + 2 ferrocytochrome b5 + 2 H+ + O2 = 7alpha,12alpha-dihydroxycholest-4-en-3-one + 2 ferricytochrome b5 + + H2O [RN:R04826]
Reaction(KEGG)
Substrate
7alpha-hydroxycholest-4-en-3-one [CPD:C05455];
ferrocytochrome b5 [CPD:C00999];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
7alpha,12alpha-dihydroxycholest-4-en-3-one;
ferricytochrome b5 [CPD:C00996];
+ H2O
Comment
A P-450 heme-thiolate protein. Requires EC 1.6.2.4, NADPH---hemoprotein reductase and cytochrome b5 for maximal activity. This enzyme is important in bile acid biosynthesis, being responsible for the balance between the formation of cholic acid and chenodeoxycholic acid [2].
History
EC 1.14.18.8 created 2005 as EC 1.14.13.95, transferred 2015 to EC 1.14.18.8
Pathway
ec00120  Primary bile acid biosynthesis
ec01100  Metabolic pathways
Orthology
K07431  sterol 12-alpha-hydroxylase
Genes
HSA: 1582(CYP8B1)
PTR: 736962(CYP8B1)
PPS: 100969929
GGO: 101147556
PON: 100433242
NLE: 100605497
MCC: 717247
MCF: 102132602
CSAB: 103227471(CYP8B1)
RRO: 104670956
RBB: 108532333
CJC: 100402181(CYP8B1)
SBQ: 101037790
MMU: 13124(Cyp8b1)
MCAL: 110302470
MPAH: 110327161
RNO: 81924(Cyp8b1)
MUN: 110554660
CGE: 100770995
NGI: 103747146
CCAN: 109691739
OCU: 100009301(CYP8B1)
TUP: 102479742
CFA: 485619
VVP: 112921604
AML: 100464768
UMR: 103682072
UAH: 113253657
ORO: 101374077
FCA: 101082004
PTG: 102956513
PPAD: 109248827
AJU: 106966675
BTA: 538964(CYP8B1)
BOM: 102278686
BIU: 109576421
BBUB: 102407955
CHX: 102173339
OAS: 101119853
SSC: 403328(CYP8B1)
CFR: 102524173
CDK: 105084606
BACU: 103000983
LVE: 103085360
OOR: 101285967
DLE: 111164311
PCAD: 102990799
ECB: 100054970
EPZ: 103559259
EAI: 106843165
MYB: 102245777
MYD: 102765424
MNA: 107524319
HAI: 109377081
DRO: 112309563
PALE: 102877747
RAY: 107519986
MJV: 108397210
LAV: 100665029
TMU: 101356001
MDO: 100026549(CYP8B)
PCW: 110217059
OAA: 103166427
GGA: 425055(CYP8B1)
MGP: 100546803
CJO: 107308837
NMEL: 110395135
APLA: 101797490
ACYG: 106036789
TGU: 100217899
LSR: 110474519
SCAN: 103812684
GFR: 102041987
FAB: 101809893
PHI: 102112501
PMAJ: 107201006
CCAE: 111925558
CCW: 104686389
ETL: 114064499
FPG: 101924073
FCH: 102052323
CLV: 102083443
EGZ: 104124585
NNI: 104021734
ACUN: 113476903
PADL: 103924714
ASN: 102374897
AMJ: 102573091(CYP8B1)
PSS: 102444673
CMY: 102934823
CPIC: 101950173
XLA: 108718630 108718631(cyp8b1.L) 379482(cyp8b1.S)
XTR: 100488015 100488168(cyp8b1)
NPR: 108788787
DRE: 445281(cyp8b1)
AMEX: 103039627
OLA: 101157008
XMA: 102227191
XCO: 114147167
PRET: 103479711
CVG: 107081670
NFU: 107383849
CSEM: 103396384
SDU: 111231161
SLAL: 111653251
HCQ: 109521313
SALP: 111956862
CMK: 103188298
RTP: 109923397
 » show all
Taxonomy
Reference
1  [PMID:1400444]
  Authors
Ishida H, Noshiro M, Okuda K, Coon MJ.
  Title
Purification and characterization of 7 alpha-hydroxy-4-cholesten-3-one 12 alpha-hydroxylase.
  Journal
J Biol Chem 267:21319-23 (1992)
Reference
2  [PMID:8943286]
  Authors
Eggertsen G, Olin M, Andersson U, Ishida H, Kubota S, Hellman U, Okuda KI, Bjorkhem I.
  Title
Molecular cloning and expression of rabbit sterol 12alpha-hydroxylase.
  Journal
J Biol Chem 271:32269-75 (1996)
DOI:10.1074/jbc.271.50.32269
  Sequence
[ocu:100009301]
Reference
3  [PMID:12543708]
  Authors
Russell DW.
  Title
The enzymes, regulation, and genetics of bile acid synthesis.
  Journal
Annu Rev Biochem 72:137-74 (2003)
DOI:10.1146/annurev.biochem.72.121801.161712
  Sequence
[hsa:1582] [mmu:13124]
Other DBs
ExplorEnz - The Enzyme Database: 1.14.18.8
IUBMB Enzyme Nomenclature: 1.14.18.8
ExPASy - ENZYME nomenclature database: 1.14.18.8
BRENDA, the Enzyme Database: 1.14.18.8

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