KEGG   ENZYME: 1.14.19.16
Entry
EC 1.14.19.16               Enzyme                                 

Name
linoleoyl-lipid Delta12 conjugase (11E,13Z-forming);
Fac (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
Sysname
linoleoyl-lipid,ferrocytochrome-b5:oxygen 11,14 allylic oxidase (11E,13Z-forming)
Reaction(IUBMB)
a linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+ = a (9Z,11E,13Z)-octadeca-9,11,13-trienoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
Substrate
linoleoyl-[glycerolipid] [CPD:C21529];
ferrocytochrome b5 [CPD:C00999];
O2 [CPD:C00007];
H+ [CPD:C00080]
Product
(9Z,11E,13Z)-octadeca-9,11,13-trienoyl-[glycerolipid];
ferricytochrome b5 [CPD:C00996];
H2O [CPD:C00001]
Comment
The enzyme, characterized from the plants Punica granatum (pomegranate) and Trichosanthes kirilowii (Mongolian snake-gourd), converts a single cis double bond at position 12 of linoleate incorporated into phosphatidylcholine into conjugated 11-trans and 13-cis double bonds. cf. EC 1.14.19.33, Delta12 acyl-lipid conjugase (11E,13E-forming).
History
EC 1.14.19.16 created 2015
Orthology
K21710  linoleoyl-lipid Delta12 conjugase (11E,13Z-forming)
Genes
CSV: 101217279
CMO: 103491678
Reference
1  [PMID:12354116]
  Authors
Hornung E, Pernstich C, Feussner I
  Title
Formation of conjugated Delta11Delta13-double bonds by Delta12-linoleic acid (1,4)-acyl-lipid-desaturase in pomegranate seeds.
  Journal
Eur J Biochem 269:4852-9 (2002)
DOI:10.1046/j.1432-1033.2002.03184.x
  Sequence
Reference
2  [PMID:12464604]
  Authors
Iwabuchi M, Kohno-Murase J, Imamura J
  Title
Delta 12-oleate desaturase-related enzymes associated with formation of conjugated trans-delta 11, cis-delta 13 double bonds.
  Journal
J Biol Chem 278:4603-10 (2003)
DOI:10.1074/jbc.M210748200
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.19.16
IUBMB Enzyme Nomenclature: 1.14.19.16
ExPASy - ENZYME nomenclature database: 1.14.19.16
BRENDA, the Enzyme Database: 1.14.19.16

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