KEGG ENZYME: 1.14.19.21

ENZYME: 1.14.19.21

Entry

EC 1.14.19.21               Enzyme

Name

cholesterol 7-desaturase;
nvd (gene name);
daf-36 (gene name)

Class

Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water

Sysname

cholesterol,NAD(P)H:oxygen oxidoreductase (7,8 dehydrogenating)

Reaction(IUBMB)

cholesterol + O2 + NAD(P)H + H+ = cholesta-5,7-dien-3beta-ol + NAD(P)+ + 2 H2O [RN:R11007 R11008]

Reaction(KEGG)

R11007 R11008

Substrate

cholesterol [CPD:C00187];
O2 [CPD:C00007];
NADH [CPD:C00004];
NADPH [CPD:C00005];
H+ [CPD:C00080]

Product

cholesta-5,7-dien-3beta-ol [CPD:C01164];
NAD+ [CPD:C00003];
NADP+ [CPD:C00006];
H2O [CPD:C00001]

Comment

The enzyme, characterized from several organisms including the worm Caenorhabditis elegans, the fly Drosophila melanogaster, and the ciliate Tetrahymena thermophila, is a Rieske oxygenase. In insects it participates in the the biosythesis of ecdysteroid hormones. The electrons are transferred from NAD(P)H via an electron transfer chain likely to include ferredoxin reductase and ferredoxin. The enzyme differs from regular desaturases, such as EC 1.14.19.20, 7-sterol 5(6)-desaturase, which are cytochrome b5-dependent and contain the three His-boxes that are typical to most desaturases.

History

EC 1.14.19.21 created 2015

Pathway

ec00981

Insect hormone biosynthesis

Orthology

K14938

cholesterol 7-desaturase

Genes

GGA:

427775

PCOC:

116234422

MGP:

104913710

CJO:

107321655

LMUT:

125702752

NMEL:

110407053

APLA:

101805447

ACYG:

125180915

CATA:

118257067

AFUL:

116497035

ETL:

114064164

FPG:

101919122

FCH:

102047190

CLV:

102098254

EGZ:

104124000

NNI:

104022360

PLET:

104627819

EHS:

104505667

PCAO:

104048586

ACUN:

113486626

TALA:

104361340

PADL:

103918965

AFOR:

103902418

ACHC:

115335319

HALD:

104310400

HLE:

104830020

AGEN:

126052516

GCL:

127023818

CCRI:

104154540

CSTI:

104549732

CMAC:

104480871

BREG:

104629094

FGA:

104078860

GSTE:

104264726

OHA:

104335383

NNT:

104402505

SHAB:

115617191

PGUU:

104465442

AVIT:

104267132

CVF:

104291118

CUCA:

104059142

TEO:

104381409

AAM:

106487012

AROW:

112963078

NPD:

112955952

TGT:

104573347

DNE:

112991357

SCAM:

104139811

ASN:

102371146

AMJ:

102565749

CPOO:

109314108

GGN:

109292718

PSS:

102455749

CMY:

102936991

CCAY:

125623666

DCC:

119844323

CPIC:

101940467

TST:

117867185

CABI:

116820424

MRV:

120386933

ACS:

100558783

PVT:

110087450

SUND:

121936429

PBI:

103066999

PMUR:

107302524

CTIG:

120308615

TSR:

106548819

PGUT:

117676660

APRI:

131185942

PTEX:

113444025

NSS:

113424726

VKO:

123028208

PMUA:

114590059 114592319 114592320

PRAF:

128409391

ZVI:

118080215

HCG:

128339121

GJA:

107120404

STOW:

125442159

EMC:

129342293

XLA:	108698103(XB5992703.L) 779348(XB5992703.S)

XTR:

100216141

NPR:

108795927

RTEM:

120913993

BBUF:

120977408 120977409

BGAR:

122919655 122946149 122946150 122946762

MUO:

115472163

GSH:

117367721

DRE:	436885(zgc:92275)

SRX:	107739715 107754619

SANH:

107662064 107665231

SGH:

107584623

CCAR:

109045525

CAUA:

113063929

CGIB:

127941321 127985608(zgc:92275)

PTET:

122326366(zgc:92275)

LROH:

127153055(zgc:92275)

OMC:	131528618(zgc:92275)

PPRM:

120490655(zgc:92275)

MAMB:

125252778(zgc:92275)

CIDE:

127503337 127525455

MASI:

127428623(zgc:92275) 127431275

TROS:

130570563(zgc:92275)

IPU:	108279335(zgc:92275)

PHYP:

113535713

SMEO:

124397555(zgc:92275)

TFD:	113646467(zgc:92275)

AMEX:

103022783

CMAO:

118820347(zgc:92275)

EEE:

113573769

CHAR:

105902521(zgc:92275)

TRU:

101069484

TFS:	130517529(zgc:92275)

TNG:	GSTEN00005122G001

LCO:

104936331

NCC:

104958128

CGOB:

115016994

ELY:	117269523(zgc:92275)

EFO:	125906002(zgc:92275)

PLEP:

121960166(zgc:92275)

SLUC:

116034845(zgc:92275)

ECRA:

117959585(zgc:92275)

ESP:

116704543

PFLV:

114571346

GAT:	120831891(zgc:92275)

PPUG:

119226249(zgc:92275)

AFB:	129102857(zgc:92275)

CLUM:

117740739(zgc:92275)

MSAM:

119914737(zgc:92275)

SCHU:

122865580(zgc:92275)

CUD:	121524811(zgc:92275)

ALAT:

119030527(zgc:92275)

MZE:

101475379

ONL:

100702604

OAU:	116319597(zgc:92275)

OLA:	101170113 105358384

OML:	112162855(zgc:92275)

XMA:

102227123

XCO:

114150182

XHE:

116724557

PRET:

103473999

PFOR:

103154387

PLAI:

106937655

PMEI:

106907543

GAF:	122819185(zgc:92275)

CVG:

107102253

CTUL:

119772015(zgc:92275)

GMU:	124873052(zgc:92275)

NFU:

107375133

KMR:	108241378(zgc:92275)

ALIM:

106513553 106533739

NWH:	119424478(zgc:92275)

AOCE:

111571560

MCEP:

124996303(zgc:92275)

CSEM:

103389306

POV:

109640312

SSEN:

122758614(zgc:92275)

HHIP:

117771622(zgc:92275)

HSP:	118125834(zgc:92275)

LCF:	108894422(zgc:92275)

SDU:

111238492

SLAL:

111650147

XGL:	120806669(zgc:92275)

SSCV:

125979739(zgc:92275)

BPEC:

110172811

BSPL:

114866897(zgc:92275)

SASA:

106565467

STRU:

115159509

OTW:	112219356(zgc:92275)

OMY:	110523638(zgc:92275)

OGO:	124048900(zgc:92275)

ONE:

115123474

OKI:	109868855 109873612

SALP:

111961562

SNH:	120025543(zgc:92275)

CCLU:

121582460

ELS:

105024189

SFM:

108922591

PKI:

111853969

AANG:

118206903(zgc:92275)

LOC:

102691906

PSPA:

121306732(zgc:92275)

ARUT:

117396731(zgc:92275) 117974032

PSEX:

120542954(zgc:92275)

LCM:

102356630

CMK:	103183010(zgc:92275)

RTP:

109938749

CPLA:

122564641(zgc:92275)

LERI:

129712167(zgc:92275)

BFO:	118414807 118420780

BBEL:

109466201 109482902

CIN:	100176071 100177386

SCLV:

120327085 120337189 120337310 120337807 120343680

SPU:

592788

LPIC:

129277485 129277488

APLC:

110977513 110977515 110977953 110977954 110980559 110985648 110986001

AJC:

117100009

SKO:

100374996

DME:	Dmel_CG40050(nvd)

DER:

26527012

DYA:	Dyak_GE28326

DSR:

110187751

DPO:

26533291

DPE:

113565812

DMN:

108153908

DWI:

6648040

DGR:

116806032

DMO:	Dmoj_GI25582

DAZ:

108621623

DNV:

108649185

DHE:

111598510

CCAT:

101462794

TDA:

119677549

SCAC:

106084232

LSQ:

119600883

HIS:

119656808

AGA:	AgaP_AGAP001038

ACOZ:

120957273

AARA:

120906649

AMER:

121596536

ASTE:

118502971

AFUN:

125771269

AALI:

118459676

AAG:

5574523

AALB:

109405340 115255620

CQU:	CpipJ_CPIJ006320

BCOO:

119069940 119075266

AME:

107965828

ACER:

108001271

ALAB:

122720233

ADR:

102679678

AFLR:

100864931

BIM:

100745011

BBIF:

117204682

BVK:

117232584

BVAN:

117156288

BTER:

100649007

BAFF:

126922147

BPYO:

122567532

CCAL:

108631726

OBB:

114876484

MGEN:

117224230

NMEA:

116426513

CGIG:

122399135

SOC:

105204704

MPHA:

105836516

AEC:

105154519

ACEP:

105621110 105621113

PBAR:

105430807

VEM:

105563098

HST:

105187219

DQU:

106741859

CFO:

105251516

FEX:

115236188

LHU:

105674672

PGC:

109856152

OBO:

105277313

PCF:

106783952

PFUC:

122524986

VPS:

122627660

NVI:	100119735 100119998

CSOL:

105367830

TPRE:

106651441

LHT:

122498793

LBD:

127279370

MDL:	103572543 103573967

CGLO:

123262462

FAS:

105262869

DAM:

107037320

AGIF:

122855124

CINS:

118073524

CCIN:

107270451

DSM:

124413684

NPT:

124222514

NFB:

124184555

NLO:

107225306

NVG:

124307222

BMOR:

733067(Nvd)

BMAN:

114251510

MSEX:

115451343

DPL:	KGM_215135

BANY:

112048798

MJU:

123879537

NIQ:

126779861

VCD:

124542283

MCIX:

123666034

PMAC:

106708229 106708230

PPOT:

106105902

PXU:

106126060

PRAP:

110997305

PBX:

123715475

PNAP:

125061310

ZCE:	119838466 119838565

CCRC:

123704539 123704683 123704684

LSIN:

126973934

HAW:

110382619

HZE:

124644511

TNL:

113509023

SLIU:

111360496

OFU:	114360096 114362450

PXY:

105380845

PGW:

126379673

CFEL:

113367651 113367652

CCRN:

123296378

API:

100162250

DNX:

107165578

AGS:

114126496

RMD:

113551662

BTAB:

109029997 109030017 109030061 109030063 109030501 109030523 109032522 109036892 109043093

DCI:	103506558 103506559

CLEC:

106673050

HHAL:

106685329

NLU:

111056285

HVI:

124356117

PHU:	Phum_PHUM458690

FOC:

113204432

TPAL:

117641049

ZNE:

110826966

CSEC:

111867050

SGRE:

126365945

IEL:

124155461

FCD:	110842436 110846580 110846657 110848904 110854554 110854966 110857831 110861595

DPX:	DAPPUDRAFT_18823 DAPPUDRAFT_308492 DAPPUDRAFT_311567

DMK:	116926277 116932277

DPZ:	124315332 124335932

PVM:

113823163

PJA:

122253458

PCHN:

125026412

PMOO:

119580273

HAME:

121872029

PCLA:

123757872

PTRU:

123515039

ESN:

126982508

HAZT:

108673947

EAF:	111708930 111716479

LSM:

121127772

PPOI:

119111722

ISC:	IscW_ISCW001214 IscW_ISCW001215 IscW_ISCW001216 IscW_ISCW005748 IscW_ISCW009663 IscW_ISCW009664 IscW_ISCW009665 IscW_ISCW010160 IscW_ISCW010161 IscW_ISCW012052 IscW_ISCW015282 IscW_ISCW021241

DSV:	119437085 119437095 119437107 119437150 119451449 119451504

RSAN:

119379265 119386907 119398458 119398507

RMP:	119161394 119161395 119162163 119162376

TUT:

107371675

DPTE:

113795498

DFR:

124489826

CSCU:

111614253 111614257 111614720 111615414 111617134 111621076 111621087 111623385 111629464 111629490 111630269 111630271 111633589 111633591 111633595 111636474 111637897 111638991 111639843 111640992 111641705 111641850

UDV:	129221062 129224184

SDM:

118201532

LPOL:

106459473

CEL:	CELE_C12D8.5(daf-36)

CBR:	CBG_09553(Cbr-daf-36)

CRQ:	GCK72_017584 GCK72_025847

BMY:	BM_BM11695(Bma-daf-36)

LOA:	LOAG_18474

NAI:	NECAME_15984

HRO:	HELRODRAFT_107375 HELRODRAFT_87564

LGI:	LOTGIDRAFT_130105

PCAN:

112575618

BGT:

106064009

GAE:	121382624 121384285

HRF:	124138371 124138372

HRJ:	124267628 124267629

CRG:	105340983 105340985

CVN:	111123313 111123314 111126872 111127059

CANU:

128155544 128156847

OED:	125681243 125681336

MYI:

110464414

PMAX:

117321955

MCAF:

127728652

MMER:

123557356 123557370

DPOL:

127837758 127856245

OBI:

106883186

OSN:

115223821

LAK:	106164789 106177872

NVE:

5515211

EPA:

110251413

ATEN:

116304131

ADF:

107344862

AMIL:

114947222

PDAM:

113681662

SPIS:

111326686

DGT:

114530380

XEN:

124439673

AQU:

100634894

NCOL:

116268054

SRE:	PTSG_07043

ACAN:

ACA1_048400 ACA1_164170

PTM:	GSPATT00025959001 GSPATT00032008001 GSPATT00035183001 GSPATT00035190001 GSPATT00036085001

SMIN:

v1.2.004636.t1(symbB.v1.2.004636.t1)

AAF:	AURANDRAFT_31508

NGR:	NAEGRDRAFT_78012 NAEGRDRAFT_78121

FAU:	Fraau_1241

HALC:

EY643_18085

SOK:	D0B54_08475

BMJ:	BMULJ_05577

BMU:

Bmul_5949

BMK:

DM80_6398

VBO:	CKY39_30055

SRC:	M271_41250

SMAL:

SMALA_1177

SAUO:

BV401_09655

SGV:	B1H19_02775

TCU:

Tcur_2516

PSEA:

WY02_22000

PSEH:

XF36_05215

AYM:	YM304_00970

TER:

Tery_0576

» show all

Reference

1 [PMID:21632547]

Authors

Yoshiyama-Yanagawa T, Enya S, Shimada-Niwa Y, Yaguchi S, Haramoto Y, Matsuya T, Shiomi K, Sasakura Y, Takahashi S, Asashima M, Kataoka H, Niwa R

Title

The conserved Rieske oxygenase DAF-36/Neverland is a novel cholesterol-metabolizing enzyme.

Journal

J Biol Chem 286:25756-62 (2011)
DOI:10.1074/jbc.M111.244384

Sequence

[cel:CELE_C12D8.5]

Reference

2 [PMID:21749634]

Authors

Wollam J, Magomedova L, Magner DB, Shen Y, Rottiers V, Motola DL, Mangelsdorf DJ, Cummins CL, Antebi A

Title

The Rieske oxygenase DAF-36 functions as a cholesterol 7-desaturase in steroidogenic pathways governing longevity.

Journal

Aging Cell 10:879-84 (2011)
DOI:10.1111/j.1474-9726.2011.00733.x

Sequence

[cel:CELE_C12D8.5]

Reference

3 [PMID:23603937]

Authors

Najle SR, Nusblat AD, Nudel CB, Uttaro AD

Title

The Sterol-C7 desaturase from the ciliate Tetrahymena thermophila is a Rieske Oxygenase, which is highly conserved in animals.

Journal

Mol Biol Evol 30:1630-43 (2013)
DOI:10.1093/molbev/mst076

Reference

4 [PMID:24244885]

Authors

Barry SM, Challis GL

Title

Mechanism and Catalytic Diversity of Rieske Non-Heme Iron-Dependent Oxygenases.

Journal

ACS Catal 3:2362–2370 (2013)
DOI:10.1021/cs400087p

Other DBs

ExplorEnz - The Enzyme Database:

1.14.19.21

IUBMB Enzyme Nomenclature:

1.14.19.21

ExPASy - ENZYME nomenclature database:

1.14.19.21

BRENDA, the Enzyme Database:

1.14.19.21

DBGET integrated database retrieval system