KEGG   ENZYME: 1.14.19.21
Entry
EC 1.14.19.21               Enzyme                                 
Name
cholesterol 7-desaturase;
nvd (gene name);
daf-36 (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
Sysname
cholesterol,NAD(P)H:oxygen oxidoreductase (7,8 dehydrogenating)
Reaction(IUBMB)
cholesterol + O2 + NAD(P)H + H+ = cholesta-5,7-dien-3beta-ol + NAD(P)+ + 2 H2O [RN:R11007 R11008]
Reaction(KEGG)
R11007 R11008
Substrate
cholesterol [CPD:C00187];
O2 [CPD:C00007];
NADH [CPD:C00004];
NADPH [CPD:C00005];
H+ [CPD:C00080]
Product
cholesta-5,7-dien-3beta-ol [CPD:C01164];
NAD+ [CPD:C00003];
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Comment
The enzyme, characterized from several organisms including the worm Caenorhabditis elegans, the fly Drosophila melanogaster, and the ciliate Tetrahymena thermophila, is a Rieske oxygenase. In insects it participates in the the biosythesis of ecdysteroid hormones. The electrons are transferred from NAD(P)H via an electron transfer chain likely to include ferredoxin reductase and ferredoxin. The enzyme differs from regular desaturases, such as EC 1.14.19.20, 7-sterol 5(6)-desaturase, which are cytochrome b5-dependent and contain the three His-boxes that are typical to most desaturases.
History
EC 1.14.19.21 created 2015
Pathway
ec00981  Insect hormone biosynthesis
Orthology
K14938  cholesterol 7-desaturase
Genes
GGA427775
PCOC116234422
MGP104913710
CJO107321655
NMEL110407053
APLA101805447
AFUL116497035
ETL114064164
FPG101919122
FCH102047190
CLV102098254
EGZ104124000
NNI104022360
ACUN113486626
TALA104361340
PADL103918965
ACHC115335319
AAM106487012
AROW112963078
NPD112955952
DNE112991357
ASN102371146
AMJ102565749
CPOO109314108
GGN109292718
PSS102455749
CMY102936991
CPIC101940467
TST117867185
CABI116820424
MRV120386933
ACS100558783
PVT110087450
SUND121936429
PBI103066999
PMUR107302524
TSR106548819
PGUT117676660
VKO123028208
PMUA114590059 114592319 114592320
ZVI118080215
GJA107120404
XLA108698103(XB5992703.L) 779348(XB5992703.S)
XTR100216141
NPR108795927
RTEM120913993
BBUF120977408 120977409
BGAR122919655 122946149 122946150 122946762
DRE436885(zgc:92275)
SRX107739715 107754619
SANH107662064 107665231
SGH107584623
CCAR109045525
CAUA113063929
IPU108279335(zgc:92275)
PHYP113535713
SMEO124397555(zgc:92275)
AMEX103022783
EEE113573769
TRU101069484
TNGGSTEN00005122G001
LCO104936331
NCC104958128
CGOB115016994
ELY117269523(zgc:92275)
PLEP121960166(zgc:92275)
SLUC116034845(zgc:92275)
ECRA117959585(zgc:92275)
PFLV114571346
GAT120831891(zgc:92275)
PPUG119226249(zgc:92275)
MSAM119914737(zgc:92275)
CUD121524811(zgc:92275)
MZE101475379
ONL100702604
OAU116319597(zgc:92275)
OLA101170113 105358384
OML112162855(zgc:92275)
XMA102227123
XCO114150182
XHE116724557
PRET103473999
PFOR103154387
PLAI106937655
PMEI106907543
GAF122819185(zgc:92275)
CVG107102253
CTUL119772015(zgc:92275)
GMU124873052(zgc:92275)
NFU107375133
KMR108241378(zgc:92275)
ALIM106513553 106533739
NWH119424478(zgc:92275)
AOCE111571560
CSEM103389306
POV109640312
SSEN122758614(zgc:92275)
HHIP117771622(zgc:92275)
LCF108894422
SDU111238492
SLAL111650147
XGL120806669(zgc:92275)
BPEC110172811
SASA106565467
OTW112219356(zgc:92275)
OMY110523638(zgc:92275)
OGO124048900(zgc:92275)
ONE115123474
SALP111961562
SNH120025543(zgc:92275)
ELS105024189
SFM108922591
PKI111853969
AANG118206903(zgc:92275)
LOC102691906
PSPA121306732(zgc:92275)
ARUT117396731(zgc:92275) 117974032
LCM102356630
CMK103183010(zgc:92275)
RTP109938749
BFO118414807 118420780
BBEL109466201 109482902
CIN100176071 100177386
SCLV120327085 120337189 120337310 120337807 120343680
SPU592788
APLC110977513 110977515 110977953 110977954 110980559 110985648 110986001
SKO100374996
DMEDmel_CG40050(nvd)
DER26527012
DYADyak_GE28326
DSR110187751
DPODpse_GA31871
DPE113565812
DMN108153908
DWI6648040
DGR116806032
DMODmoj_GI25582
DAZ108621623
DNV108649185
DHE111598510
CCAT101462794
SCAC106084232
LCQ111688255
AGAAgaP_AGAP001038
ACOZ120957273
AARA120906649
AAG5574523
AALB109405340 115255620
CQUCpipJ_CPIJ006320
AME107965828
ACER108001271
ALAB122720233
BIM100745011
BBIF117204682
BVK117232584
BVAN117156288
BTER100649007
BPYO122567532
CCAL108631726
OBB114876484
MGEN117224230
NMEA116426513
CGIG122399135
SOC105204704
MPHA105836516
AEC105154519
ACEP105621110 105621113
PBAR105430807
VEM105563098
HST105187219
DQU106741859
CFO105251516
FEX115236188
LHU105674672
PGC109856152
OBO105277313
PCF106783952
PFUC122524986
VPS122627660
NVI100119735 100119998
CSOL105367830
TPRE106651441
MDL103572543 103573967
CGLO123262462
FAS105262869
DAM107037320
AGIF122855124
CCIN107270451
BMOR733067(Nvd)
BMAN114251510
MSEX115451343
DPLKGM_215135
BANY112048798
PMAC106708229 106708230
PPOT106105902
PXU106126060
PRAP110997305
ZCE119838466 119838565
HAW110382619 110383239
TNL113509023
OFU114360096 114362450
PXY105380845
API100162250
DNX107165578
AGS114126496
RMD113551662
BTAB109029997 109030017 109030061 109030063 109030501 109030523 109032522 109036892 109043093
DCI103506558 103506559
CLEC106673050
HHAL106685329
NLU111056285
PHUPhum_PHUM458690
FOC113204432
ZNE110826966
CSEC111867050
FCD110842436 110846580 110846657 110848904 110854554 110854966 110857831 110861595
DPXDAPPUDRAFT_18823 DAPPUDRAFT_308492 DAPPUDRAFT_311567
DMK116926277 116932277
PVM113823163
PJA122253458
HAME121872029
PCLA123757872
PTRU123515039
HAZT108673947
EAF111708930 111716479
ISCIscW_ISCW001214 IscW_ISCW001215 IscW_ISCW001216 IscW_ISCW005748 IscW_ISCW009663 IscW_ISCW009664 IscW_ISCW009665 IscW_ISCW010160 IscW_ISCW010161 IscW_ISCW012052 IscW_ISCW015282 IscW_ISCW021241
DSV119437085 119437095 119437107 119437136 119437150 119451449 119451504
RSAN119379265 119386907 119398458
RMP119161394 119161395 119162163 119162376
TUT107371675
DPTE113795498
DFR124489826
CSCU111614253 111614257 111614720 111615414 111617134 111621076 111621087 111623385 111629464 111629490 111630269 111630271 111633589 111633591 111633595 111636474 111637897 111638991 111639843 111640992 111641705 111641850
SDM118201532
CELCELE_C12D8.5(daf-36)
CBRCBG_09553(Cbr-daf-36)
BMYBM_BM11695(Bma-daf-36)
LOALOAG_18474
NAINECAME_15984
HROHELRODRAFT_107375 HELRODRAFT_87564
LGILOTGIDRAFT_130105
PCAN112575618
BGT106064009
GAE121382624 121384285
HRF124138371 124138372
HRJ124267628 124267629
CRG105340983 105340985
MYI110464414
PMAX117321955
MMER123528575 123557356 123557370
OBI106883186
OSN115223821
LAK106164789 106177872
NVE5515211
EPA110251413
ATEN116304131
ADF107344862
AMIL114947222
PDAM113681662
SPIS111326686
DGT114530380
XEN124439673
AQU100634894
NCOL116268054
SREPTSG_07043
ACANACA1_048400 ACA1_164170
PTMGSPATT00025959001 GSPATT00032008001 GSPATT00035183001 GSPATT00035190001 GSPATT00036085001
SMINv1.2.004636.t1(symbB.v1.2.004636.t1)
AAFAURANDRAFT_31508
NGRNAEGRDRAFT_78012 NAEGRDRAFT_78121
FAUFraau_1241
PCHEY04_14500
HALCEY643_18085
SOKD0B54_08475
BCNBcen_1322
BCHBcen2424_6507
BMJBMULJ_05577
BMUBmul_5949
BMKDM80_6398
VBOCKY39_30055
SRCM271_41250
SMALSMALA_1177
SAUOBV401_09655
SGVB1H19_02775
TCUTcur_2516
PSEAWY02_22000
PSEHXF36_05215
AYMYM304_00970
TERTery_0576
 » show all
Reference
1  [PMID:21632547]
  Authors
Yoshiyama-Yanagawa T, Enya S, Shimada-Niwa Y, Yaguchi S, Haramoto Y, Matsuya T, Shiomi K, Sasakura Y, Takahashi S, Asashima M, Kataoka H, Niwa R
  Title
The conserved Rieske oxygenase DAF-36/Neverland is a novel cholesterol-metabolizing enzyme.
  Journal
J Biol Chem 286:25756-62 (2011)
DOI:10.1074/jbc.M111.244384
  Sequence
Reference
2  [PMID:21749634]
  Authors
Wollam J, Magomedova L, Magner DB, Shen Y, Rottiers V, Motola DL, Mangelsdorf DJ, Cummins CL, Antebi A
  Title
The Rieske oxygenase DAF-36 functions as a cholesterol 7-desaturase in steroidogenic pathways governing longevity.
  Journal
Aging Cell 10:879-84 (2011)
DOI:10.1111/j.1474-9726.2011.00733.x
  Sequence
Reference
3  [PMID:23603937]
  Authors
Najle SR, Nusblat AD, Nudel CB, Uttaro AD
  Title
The Sterol-C7 desaturase from the ciliate Tetrahymena thermophila is a Rieske Oxygenase, which is highly conserved in animals.
  Journal
Mol Biol Evol 30:1630-43 (2013)
DOI:10.1093/molbev/mst076
Reference
4  [PMID:24244885]
  Authors
Barry SM, Challis GL
  Title
Mechanism and Catalytic Diversity of Rieske Non-Heme Iron-Dependent Oxygenases.
  Journal
ACS Catal 3 (2013)
DOI:10.1021/cs400087p
Other DBs
ExplorEnz - The Enzyme Database: 1.14.19.21
IUBMB Enzyme Nomenclature: 1.14.19.21
ExPASy - ENZYME nomenclature database: 1.14.19.21
BRENDA, the Enzyme Database: 1.14.19.21

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