KEGG   ENZYME: 1.14.19.22Help
Entry
EC 1.14.19.22               Enzyme                                 

Name
acyl-lipid omega-6 desaturase (cytochrome b5);
oleate desaturase (ambiguous);
linoleate synthase (ambiguous);
oleoyl-CoA desaturase (incorrect);
oleoylphosphatidylcholine desaturase (ambiguous);
phosphatidylcholine desaturase (ambiguous);
n-6 desaturase (ambiguous);
FAD2 (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
BRITE hierarchy
Sysname
1-acyl-2-oleoyl-sn-glycero-3-phosphocholine,ferrocytochrome-b5:oxygen oxidoreductase (12,13 cis-dehydrogenating)
Reaction(IUBMB)
an oleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+ = a linoleoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
Substrate
oleoyl-[glycerolipid];
ferrocytochrome b5 [CPD:C00999];
O2 [CPD:C00007];
H+ [CPD:C00080]
Product
linoleoyl-[glycerolipid] [CPD:C21529];
ferricytochrome b5 [CPD:C00996];
H2O [CPD:C00001]
Comment
This microsomal enzyme introduces a cis double bond in fatty acids attached to lipid molecules at a location 6 carbons away from the methyl end of the fatty acid. The distance from the carboxylic acid end of the molecule does not affect the location of the new double bond. The most common substrates are oleoyl groups attached to either the sn-1 or sn-2 position of the glycerol backbone in phosphatidylcholine. cf. EC 1.14.19.23, acyl-lipid omega-6 desaturase (ferredoxin).
History
EC 1.14.19.22 created 1984 as EC 1.3.1.35, transferred 2015 to EC 1.14.19.22
Orthology
K10256  omega-6 fatty acid desaturase / acyl-lipid omega-6 desaturase (Delta-12 desaturase)
Genes
BTAB: 109031516 109034286 109037327 109038609 109038610 109038611 109043360 109043386 109043387 109043419 109043487 109043488 109043505
NVE: 5512103
EPA: 110243700
ADF: 107344154
AMIL: 114948134 114973367
PDAM: 113667909 113682750
SPIS: 111329485 111342409
ATH: AT3G12120(FAD2)
ALY: 9318834
CRB: 17890721
BRP: 103870276
BOE: 106295108
RSZ: 108862913
CPAP: 110811677
CIT: 102627239
GMX: 100780912(GMFAD2-2D) 100805040(GMFAD2-2B) 100805777(FAD2-1B) 100809290(GMFAD2-2A) 102662570 547814(FAD2-1A) 547815(FAD2-2) 732617(FAD2-3)
LJA: Lj1g3v4317650.1(Lj1g3v4317650.1) Lj5g3v2237610.1(Lj5g3v2237610.1) Lj5g3v2237610.2(Lj5g3v2237610.2)
ZJU: 107432351
CSV: 101222623
CMO: 103486770
MCHA: 111024845
RCU: 8267086
JCU: 105646514
POP: 7479530
VVI: 100252129
SIND: 105159686 105163532(FAD2)
OSA: 4330523
DOSA: Os02t0716500-01(Os02g0716500) Os07t0416700-00(Os07g0416700)
OBR: 102719739
ATS: 109756354(LOC109756354) 109768827(LOC109768827) 109774922(LOC109774922) 109774938(LOC109774938) 109781985(LOC109781985)
ZMA: 100282744
PDA: 103713931
EGU: 105050201
MUS: 103980416
ATR: 18439780
MNG: MNEG_7806
ERC: Ecym_2769
TDL: TDEL_0E01510(TDEL0E01510)
PIC: PICST_72518(ODD1)
CAL: CAALFM_C601110WA(FAD2)
SLB: AWJ20_1033(ODE1)
NCR: NCU02209(ods)
NTE: NEUTE1DRAFT86398(NEUTE1DRAFT_86398)
MGR: MGG_01985
SSCK: SPSK_00128
MAW: MAC_06628
MAJ: MAA_07889
CMT: CCM_06866
ANI: AN1037.2
ANG: ANI_1_742074(An08g05160)
ABE: ARB_01637
TVE: TRV_05152
PTE: PTT_13901
CNE: CND02750
CNB: CNBD3600
LBC: LACBIDRAFT_292603(FAD12-5)
ABP: AGABI1DRAFT113988(AGABI1DRAFT_113988)
ABV: AGABI2DRAFT193825(AGABI2DRAFT_193825)
MGL: MGL_1345
MRT: MRET_4015
NGD: NGA_0081700(DES)
TCR: 429257.20
 » show all
Taxonomy
Reference
1  [PMID:166662]
  Authors
Pugh EL, Kates M.
  Title
Characterization of a membrane-bound phospholipid desaturase system of candida lipolytica.
  Journal
Biochim Biophys Acta 380:442-53 (1975)
DOI:10.1016/0005-2760(75)90112-5
Reference
2  [PMID:475773]
  Authors
Slack CR, Roughan PG, Browse J.
  Title
Evidence for an oleoyl phosphatidylcholine desaturase in microsomal preparations from cotyledons of safflower (Carthamus tinctorius) seed.
  Journal
Biochem J 179:649-56 (1979)
DOI:10.1042/bj1790649
Reference
3  [PMID:710426]
  Authors
Stymne S, Appelqvist LA.
  Title
The biosynthesis of linoleate from oleoyl-CoA via oleoyl-phosphatidylcholine in microsomes of developing safflower seeds.
  Journal
Eur J Biochem 90:223-9 (1978)
DOI:10.1111/j.1432-1033.1978.tb12594.x
Reference
4  [PMID:2264826]
  Authors
Smith MA, Cross AR, Jones OT, Griffiths WT, Stymne S, Stobart K
  Title
Electron-transport components of the 1-acyl-2-oleoyl-sn-glycero-3-phosphocholine  delta 12-desaturase (delta 12-desaturase) in microsomal preparations from developing safflower (Carthamus tinctorius L.) cotyledons.
  Journal
Biochem J 272:23-9 (1990)
DOI:10.1042/bj2720023
Reference
5  [PMID:1989527]
  Authors
Kearns EV, Hugly S, Somerville CR
  Title
The role of cytochrome b5 in delta 12 desaturation of oleic acid by microsomes of safflower (Carthamus tinctorius L.).
  Journal
Arch Biochem Biophys 284:431-6 (1991)
DOI:10.1016/0003-9861(91)90319-E
Reference
6  [PMID:1730697]
  Authors
Miquel M, Browse J
  Title
Arabidopsis mutants deficient in polyunsaturated fatty acid synthesis. Biochemical and genetic characterization of a plant oleoyl-phosphatidylcholine desaturase.
  Journal
J Biol Chem 267:1502-9 (1992)
Other DBs
ExplorEnz - The Enzyme Database: 1.14.19.22
IUBMB Enzyme Nomenclature: 1.14.19.22
ExPASy - ENZYME nomenclature database: 1.14.19.22
BRENDA, the Enzyme Database: 1.14.19.22
CAS: 72536-70-0

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