KEGG   ENZYME: 1.14.19.22
Entry
EC 1.14.19.22               Enzyme                                 
Name
acyl-lipid omega-6 desaturase (cytochrome b5);
oleate desaturase (ambiguous);
linoleate synthase (ambiguous);
oleoyl-CoA desaturase (incorrect);
oleoylphosphatidylcholine desaturase (ambiguous);
phosphatidylcholine desaturase (ambiguous);
n-6 desaturase (ambiguous);
FAD2 (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
Sysname
1-acyl-2-oleoyl-sn-glycero-3-phosphocholine,ferrocytochrome-b5:oxygen oxidoreductase (12,13 cis-dehydrogenating)
Reaction(IUBMB)
an oleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+ = a linoleoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
Substrate
oleoyl-[glycerolipid];
ferrocytochrome b5 [CPD:C00999];
O2 [CPD:C00007];
H+ [CPD:C00080]
Product
linoleoyl-[glycerolipid] [CPD:C21529];
ferricytochrome b5 [CPD:C00996];
H2O [CPD:C00001]
Comment
This microsomal enzyme introduces a cis double bond in fatty acids attached to lipid molecules at a location 6 carbons away from the methyl end of the fatty acid. The distance from the carboxylic acid end of the molecule does not affect the location of the new double bond. The most common substrates are oleoyl groups attached to either the sn-1 or sn-2 position of the glycerol backbone in phosphatidylcholine. cf. EC 1.14.19.23, acyl-lipid omega-6 desaturase (ferredoxin).
History
EC 1.14.19.22 created 1984 as EC 1.3.1.35, transferred 2015 to EC 1.14.19.22
Orthology
K10256  omega-6 fatty acid desaturase / acyl-lipid omega-6 desaturase (Delta-12 desaturase)
Genes
AJM119047578
BCOO119067137 119073867 119077976 119084236 119085821
BTAB109031516 109034286 109037327 109038609 109038610 109038611 109043360 109043386 109043387 109043419 109043487 109043488 109043505
SGRE126324549
PVUL126811615
GAE121374992 121375004
HRF124116169
HRJ124268532 124268533
OSN115209645
LJP135479498 135479727
NVE5512103
EPA110243700
ATEN116303763
ADF107344154
AMIL114948134 114973367
PDAM113667909 113682750
SPIS111329485 111342409
ATHAT3G12120(FAD2)
ALY9318834
CRB17890721
CSAT104745425 104764975 104776214
EUSEUTSA_v10020910mg
BRP103870276
BNA106417584 106452409 106452523
BOE106295108
RSZ108862913 130497099 130504985
THJ104799296 104826770
CPAP110811677
CIT102611163 102627239
CICCICLE_v10011949mg CICLE_v10015614mg
PVY116125738 116136611
MINC123198158 123214921 123225237 123228010
TCC18589688 18599101
GRA105761671 105783314 105785452 105785453
GHI107901973 107921152 107921339 107924178 107934594 107941903 107945411 121212649
GAB108450424 108473778 108480547 108482302
HSYR120153918 120158105 120183510 120183934
DZI111295947 111304019 111311572
EGR104433804 104445836
GMX100780912(FAD2-2D) 100805040(FAD2-2B) 100805777(FAD2-1B) 100809290(FAD2-2A) 102662570 547814(FAD2-1A) 547815(FAD2-2) 732617(FAD2-3)
GSJ114367074 114370405 114400352 114401640 114406779
PVUPHAVU_001G142100g PHAVU_007G0234001 PHAVU_007G259500g
VRA106757695 106770720 106771256
VAR108323476 108325188 108325364
VUN114174864 114189943 114190784
VUM124820456 124827773 124827774 124830459
CCAJ109794836 109795239 109796052 109796649
APRC113861993 113872359 113873750
MTR11444765 25483518 25485564
TPRA123888747 123898279 123899802
CAM101489419 101507051 101513688
PSAT127098513 127126234
VVO131621445 131621556 131636358
LJALj1g3v4317650.1(Lj1g3v4317650.1) Lj5g3v2237610.1(Lj5g3v2237610.1) Lj5g3v2237610.2(Lj5g3v2237610.2)
ADU107464693 107467337 107492893
AIP107616829 107619143 107645414
AHF112696114 112710390 112712140 112755017 112776164 112779709
LANG109328907 109331342 109345961 109346314 109347587
PCIN129301231 129303850 129319588
QSAO6P43_004880 O6P43_005841 O6P43_008751 O6P43_009477
FVE101290788 101309231
RCN112194753 112201466
PPER18769718 18774949
PMUM103339420 103341921
PAVI110763023 110770740
PDUL117633799
MDM103427045 103443127 103454260
MSYL126592667 126599950 126616101
PXB103958977 103963937 103964864
ZJU107432351
MNT21386779 21391283 21401389
CSAV115701196 115718446 115719000 115719251 115719329
CSV101222623
CMO103486770
BHJ120088910 120090365
MCHA111024845
CMAX111470178 111481665 111484610 111490747
CMOS111441899 111448352 111448353 111459302
CPEP111777569 111777619 111786454 111786608 111789928 111795654 111800320
RCU8267086
MEAN126676920
JCU105646514 105650891
HBR110635031 110638358 110650724
MESC110621646 110621802 110622513
POP18094295 7455763 7479530 7483979
PEU105111221 105131774 105134978 105134979 105134982
PALZ118043755 118049781 118049805 118049806 118049817 118052395
JRE109001694 109011954 109021930
CILL122276083 122278215 122292068
CAVE132164512 132164706 132164733 132191453
QSU112012330 112015494
QLO115952674 115977653
TWL119995107 119996264 119998754 120017269
VVI100252129 100258789
VRI117915771 117920052
SLY101248657 101250481 101255474 101259966 109119181 112941338 544107(cevi19)
SPEN107003514 107005223 107005224 107005731 107005782 107005832 107006602 107015240 107017128
SOT102579196 102579518 102579844 102580407 102581806 102584924 102585247 102593159 102596973 102597622 102597941 102598049 102598267 102598608 102600240
SSTN125856711 125858454 125862308 125862309 125866588 125866589 125866590 125866591 125866592 125866605 125872824
SDUL129870260 129871090 129871655 129887782 129888911 129891120 129891129 129891137 129894236 129902073 129902272 129902336 129902412 129902486 129902550
CANN107843168 107849596 107849612 107851251 107851421 107851434 107851473 107851685 107851744 107873633 124894986
LBB132602713 132602715 132602716 132602717 132603721 132603722 132603733 132603735 132626002 132635573
NTA107773163 107797104 107814090 107823061(FAD2) 107829678 107831844
NSY104226786 104230317 104246180
NTO104089719 104090741 104098987 104106672 104121261
NAU109215055 109215819 109216417 109216418 109218761 109235687
INI109150720 109152414 109182042
ITR116007185 116007582 116007943 116024476 116029392
SIND105159686 105163532(FAD2)
OEU111365935 111371390 111390764 111393798 111399693
EGT105951255 105955229
SSPL121747569 121751845 121794328 121798703
SMIL131000922
SHIS125188161 125205310
APAN127258679 127258874 127263751
HAN110865031 110865032 110865236 110865239 110865277 110865281 110865284 110865285 110867681 110868684 110879372 110894444 110894736 110897314 110897316 110897318 110897319 110897568 110904312 110938218 110938220 110938221 110939886 110939887 110939961 110939971
ECAD122578445 122578764 122579373 122582919 122590523 122591112 122591269 122591356 122591604 122606452 122607353 122608062 122608674 122611246
LSV111879673 111882294 111884484 111885018 111889077 111889078 111889080 111889206 111890254 111896570 111896575 111896578 111896579 111896580 111896581 111896582 111897874 111899826 111900952 111900953 111904205 111908893 111909558 111915490 111918622
CCAV112505457 112505482 112505726 112506618 112506654 112506812 112506837 112506966 112506967 112506968 112512117 112513526 112521107 112521212 112521380 112521518 112521640 112521907 112521908 112521909 112521958 112525343 112526112 112527377 112527536 112527987 112528359 112528364
DCR108193493 108194523 108197230 108197256 108197419 108198633 108198634 108198635 108198637 108198638 108199375 108202677 108204656 108204690 108207522 108210933 108211018 108212237 108217089 108217440 108218481 108218482 108218742 108218743 108219326 108220169 108220600 108220695 108221065 108221821 108221989 108222165 108222186 108223766 108223875 108224006 108225347 108227781
CSIN114267865 114282301
RVL131315019 131315020 131324332 131324333
AEW130781454 130785560
BVG104889198 104889897
SOE110785100 110800912
CQI110689244 110695943 110734516 110736134
ATRI130796765 130820812 130825871
MOF131148005 131165865 131167705 131167706 131167708
NNU104597556 104603541 104611778
MING122061917 122087967
TSS122642840 122642959
PSOM113311026 113322906
OSA4330523 4343026 4343027
DOSAOs02t0716500-01(Os02g0716500) Os07t0416700-00(Os07g0416700) Os07t0416900-01(Os07g0416900) Os07t0417200-01(Os07g0417200)
OBR102719739
OGL127762104 127780007 127780184
BDI100835723 100837786
ATS109756354 109768827 109774922 109774938 109781985
TDC119317653 119317654 119321176 119322537 119326524
TAES123128186 123130860 123130862 123134421 123134423 123138008 123145288 123145289 123145293 123145295
TUA125512392 125512434 125514359 125514360
LPER127306411 127311774 127311775
LRD124692997
SBI110435045 8060698 8066368
ZMA100191328 100273207 100282744 100381639 103631986 103640919 118475586
SITA101768137 101783555
SVS117835694 117853495
PVIR120650240 120657027 120677804 120701919
PHAI112886426 112890525 112896900
PDA103713931 103723899
EGU105050201 105061227
MUS103971019 103976995 103980416 103997712 103999878
ZOF121993200 121997168 122020013 122023662 122032659 122035107
DCT110101255 110108827
PEQ110023778 110033260
AOF109833613 109843629 109843630
MSIN131222383
NCOL116257778
ATR18439780
SMOSELMODRAFT_163663
PPP112278989 112291137
CRECHLRE_17g711150v5
VCNVOLCADRAFT_66373
MNGMNEG_7806
CVRCHLNCDRAFT_48812
CCPCHC_T00008296001
AGOAGOS_AFR589C
ERCEcym_2769
KLAKLLA0_F07095g
KMXKLMA_20392
LTHKLTH0D13640g
ZROZYRO0B07414g
TGBHG536_0E01410
TDLTDEL_0E01510(TDEL0E01510)
ZMKHG535_0F01710
PPAPAS_chr4_0052
DHADEHA2G05346g
PICPICST_72518(ODD1)
PGUPGUG_02928
SPAASPAPADRAFT_63149
LELPVL30_005361
CALCAALFM_C601110WA(FAD2)
CTPCTRG_02975
COTCORT_0F04690
CDUCD36_61210
CTEN18247366(PSN45_004733)
YLIYALI0B10153g
CLUCLUG_01582
CLUSA9F13_13g00209
CAURCJI96_0004561
SLBAWJ20_1033(ODE1)
PKZC5L36_0C09550 C5L36_0C09560
BNNFOA43_000373
BBRXBRETT_002981
OPAHPODL_03473
NCRNCU02209(ods)
NTENEUTE1DRAFT86398(NEUTE1DRAFT_86398)
SMPSMAC_03650
PBELQC761_504330(FAH12)
PPSDQC762_504330(FAH12)
PPSPQC763_504330(FAH12)
PPSAQC764_504330(FAH12)
TTTTHITE_76756
MTMMYCTH_88639
CTHRCTHT_0000240
MGRMGG_01985
PPEIPpBr36_10589
PGRIPgNI_05225
TMNUCRPA7_5047
SSCKSPSK_00128
FGRFGSG_05784
FPUFPSE_04515
FPOAFPOAC1_008748(FAH12)
FVNFVRRES_09344
FVRFVEG_05830
FOXFOXG_08835
NHENECHADRAFT_66003
FFCNCS54_00197700
FKRNCS57_00193500
FMUJ7337_004932(FAH12)
TRETRIREDRAFT_45250
TRRM419DRAFT_108308
MAW19250939(FAH12_1)
MAJMAA_07889
PCHMVFPPC_10440
CMTCCM_06866
AMUSLMH87_010184
PLJ28892358(PLICBS_000183)
PTKZJDV02_003432(FAH12)
VDAVDAG_02971
CFJCFIO01_09629
CLUPCLUP02_11556
CHIGCH63R_11053
SAPOSAPIO_CDS0643
ELAUCREL1_5610
PFYPFICI_02105
SSLSS1G_13683
BFUBCIN_01g05120 BCIN_02g06310 BCIN_13g00280
MBEMBM_04489 MBM_08304
PSCOLY89DRAFT_613354 LY89DRAFT_693845 LY89DRAFT_706947
GLZGLAREA_04493 GLAREA_09934
ANIANIA_01037
AFMAFUA_1G12530
ACTACLA_022950
NFINFIA_013020
AORAO090001000224
ANGAn08g05160
AFVAFLA_008570
ALUCAKAW2_30542S(FAH12)
ACHEACHE_30343S(FAH12)
APUUAPUU_50747S(FAH12)
PCSN7525_002119 N7525_002710
PDPPDIP_27200
POUPOX_c04082
TMFEYB26_005492
TRGTRUGW13939_09539
CIMCIMG_06871
CPWCPC735_032710
UREUREG_03614
PBLPAAG_08655
PBNPADG_07259
ABEARB_01637
TVETRV_05152
AJEHCAG_01866
BGHBDBG_00166
PNOSNOG_01471
PTEPTT_13901
BZECOCCADRAFT_95357
BSCCOCSADRAFT_255949
BORCOCMIDRAFT_99684
AALTCC77DRAFT_261211 CC77DRAFT_946470
ARABEKO05_0008621
PFJMYCFIDRAFT_72590
FFUCLAFUR5_03699
CBETCB0940_02620
BCOMBAUCODRAFT_24063
NPAUCRNP2_969
TMLGSTUM_00002898001
CNECND02750
CNBCNBD3600
CNGCNAG_01150
CGICGB_D5300C
CDEUCNBG_0718
TMSTREMEDRAFT_45585
TASAA1Q1_01239 A1Q1_06326
CCACCcaHIS019_0112250(CcaverHIS019_0112250) CcaHIS019_0508550(CcaverHIS019_0508550)
TVSTRAVEDRAFT_147014 TRAVEDRAFT_28463 TRAVEDRAFT_38834
DSQDICSQDRAFT_102286
PCOPHACADRAFT_265001
SHSSTEHIDRAFT_104093
HIRHETIRDRAFT_440056
PSQPUNSTDRAFT_141429
ADLAURDEDRAFT_115855
FMEFOMMEDRAFT_128530
GTRGLOTRDRAFT_61689
RSXRhiXN_08914 RhiXN_08917
LBCLACBIDRAFT_292603(FAD12-5)
CCICC1G_03449
ABPAGABI1DRAFT113988(AGABI1DRAFT_113988)
ABVAGABI2DRAFT193825(AGABI2DRAFT_193825)
PCUBJR316_0007061 JR316_0012985
MRRMoror_13992
MOREE1B28_012456 E1B28_013509
SCMSCHCO_02615271(SCHCODRAFT_02615271)
CPUTCONPUDRAFT_56006
SLASERLADRAFT_469833
WSEWALSEDRAFT_64045
WICJ056_000579
UMAUMAG_11270
PFPPFL1_00921
MGLMGL_1345
MRTMRET_4015
MSYMMSY001_0308
PGRPGTG_00088 PGTG_00121
PTRCPtA15_1A110 PtA15_1A111 PtA15_1A125
PSTRPst134EA_001028 Pst134EA_001035 Pst134EA_001054 Pst134EA_004855 Pst134EA_004856
MLRMELLADRAFT_70376 MELLADRAFT_75337
SREPTSG_08157
NGDNGA_0081700(DES)
TBRTb927.2.3080
TBGTbgDal_II1450
TCR429257.20
LMALMJF_33_3270
LIFLINJ_33_3420
LDOLDBPK_333420
LMILMXM_32_3270
LBZLBRM_33_3550
LPANLPMP_101260 LPMP_333400
 » show all
Reference
1  [PMID:166662]
  Authors
Pugh EL, Kates M.
  Title
Characterization of a membrane-bound phospholipid desaturase system of candida lipolytica.
  Journal
Biochim Biophys Acta 380:442-53 (1975)
DOI:10.1016/0005-2760(75)90112-5
Reference
2  [PMID:475773]
  Authors
Slack CR, Roughan PG, Browse J.
  Title
Evidence for an oleoyl phosphatidylcholine desaturase in microsomal preparations from cotyledons of safflower (Carthamus tinctorius) seed.
  Journal
Biochem J 179:649-56 (1979)
DOI:10.1042/bj1790649
Reference
3  [PMID:710426]
  Authors
Stymne S, Appelqvist LA.
  Title
The biosynthesis of linoleate from oleoyl-CoA via oleoyl-phosphatidylcholine in microsomes of developing safflower seeds.
  Journal
Eur J Biochem 90:223-9 (1978)
DOI:10.1111/j.1432-1033.1978.tb12594.x
Reference
4  [PMID:2264826]
  Authors
Smith MA, Cross AR, Jones OT, Griffiths WT, Stymne S, Stobart K
  Title
Electron-transport components of the 1-acyl-2-oleoyl-sn-glycero-3-phosphocholine  delta 12-desaturase (delta 12-desaturase) in microsomal preparations from developing safflower (Carthamus tinctorius L.) cotyledons.
  Journal
Biochem J 272:23-9 (1990)
DOI:10.1042/bj2720023
Reference
5  [PMID:1989527]
  Authors
Kearns EV, Hugly S, Somerville CR
  Title
The role of cytochrome b5 in delta 12 desaturation of oleic acid by microsomes of safflower (Carthamus tinctorius L.).
  Journal
Arch Biochem Biophys 284:431-6 (1991)
DOI:10.1016/0003-9861(91)90319-E
Reference
6  [PMID:1730697]
  Authors
Miquel M, Browse J
  Title
Arabidopsis mutants deficient in polyunsaturated fatty acid synthesis. Biochemical and genetic characterization of a plant oleoyl-phosphatidylcholine desaturase.
  Journal
J Biol Chem 267:1502-9 (1992)
Other DBs
ExplorEnz - The Enzyme Database: 1.14.19.22
IUBMB Enzyme Nomenclature: 1.14.19.22
ExPASy - ENZYME nomenclature database: 1.14.19.22
BRENDA, the Enzyme Database: 1.14.19.22
CAS: 72536-70-0

DBGET integrated database retrieval system