KEGG   ENZYME: 1.14.19.23
Entry
EC 1.14.19.23               Enzyme                                 

Name
acyl-lipid (n+3)-(Z)-desaturase (ferredoxin);
acyl-lipid omega6-desaturase (ferredoxin);
oleate desaturase (ambiguous);
linoleate synthase (ambiguous);
oleoyl-CoA desaturase (ambiguous);
oleoylphosphatidylcholine desaturase (ambiguous);
phosphatidylcholine desaturase (ambiguous);
FAD6 (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
Sysname
oleoyl-[glycerolipid],ferredoxin:oxygen oxidoreductase (12,13 cis-dehydrogenating)
Reaction(IUBMB)
an oleoyl-[glycerolipid] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+ = a linoleoyl-[glycerolipid] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
Substrate
oleoyl-[glycerolipid];
reduced ferredoxin [iron-sulfur] cluster [CPD:C00138];
O2 [CPD:C00007];
H+ [CPD:C00080]
Product
linoleoyl-[glycerolipid] [CPD:C21529];
oxidized ferredoxin [iron-sulfur] cluster [CPD:C00139];
H2O [CPD:C00001]
Comment
This plastidial enzyme is able to insert a cis double bond in monounsaturated fatty acids incorporated into glycerolipids. The enzyme introduces the new bond at a position 3 carbons away from the existing double bond, towards the methyl end of the fatty acid. The native substrates are oleoyl (18:1 Delta9) and (Z)-hexadec-7-enoyl (16:1 Delta7) groups attached to either position of the glycerol backbone in glycerolipids, resulting in the introduction of the second double bond at positions 12 and 10, respectively This prompted the suggestion that this is an omega6 desaturase. However, when acting on palmitoleoyl groups(16:1 Delta9), the enzyme introduces the second double bond at position 12 (omega4), indicating it is an (n+3) desaturase [3]. cf. EC 1.14.19.34, acyl-lipid (9+3)-(E)-desaturase.
History
EC 1.14.19.23 created 2015
Orthology
K10255  acyl-lipid omega-6 desaturase (Delta-12 desaturase)
Genes
ATH: AT4G30950(FAD6)
ALY: 9303391
CRB: 17878072
CSAT: 104717096 104727652 104730201
EUS: EUTSA_v10025201mg
BRP: 103834644 103852217
BNA: 106376162 106424191 106434448 111209020
BOE: 106322999 106336480
RSZ: 108819010 108825849 108831671 108834911
THJ: 104803563 104819907
CPAP: 110815673
CIT: 102608557
TCC: 18587855
GRA: 105782308
GHI: 107893433
GAB: 108464339
GMX: 100798793(FAD6.2) 547807(FAD6.1)
VRA: 106769001
VAR: 108323518
VUN: 114177711
CCAJ: 109810165
CAM: 101512983
LJA: Lj4g3v1983380.1(Lj4g3v1983380.1)
ADU: 107459168
AIP: 107641211
FVE: 101314027
RCN: 112186629
PPER: 18771470
PMUM: 103341259
PAVI: 110756926
MDM: 114821832
PXB: 103949078
ZJU: 107426965
CSV: 101210863
CMO: 103487358
MCHA: 111007246
RCU: 8276565
JCU: 105633504
HBR: 110663179
PEU: 105131919
JRE: 108993197
QSU: 111987099
VVI: 100248377
SLY: 101265653
SPEN: 107025896
SOT: 102590621
CANN: 107877621
NSY: 104246884
NTO: 104090666
NAU: 109213035
INI: 109166268
SIND: 105162940
OEU: 111376237
HAN: 110886589
LSV: 111909246
CCAV: 112503657
DCR: 108223364
BVG: 104900797
SOE: 110799552
OSA: 4345686
OBR: 102707796
BDI: 100833718
ATS: 109775718(LOC109775718)
SBI: 8077844
ZMA: 100101547
SITA: 101784231
PDA: 103714237
EGU: 105060194
MUS: 103974437
PEQ: 110034119
AOF: 109820085
ATR: 18447056
APRO: F751_3772
PFL: PFL_0265
PPRC: PFLCHA0_c02700(desA)
PPRO: PPC_0278
PSEM: TO66_25565
MBS: MRBBS_1979(des)
LPO: LPO_2516
LLO: LLO_1421
MEJ: Q7A_835
AEH: Mlg_1774
HHA: Hhal_0399
TVR: TVD_07725
GAI: IMCC3135_23190(des)
HCO: LOKO_02828(des)
APAC: S7S_15160
TOL: TOL_2835
OAI: OLEAN_C09380(desA)
SVA: SVA_2820
REH: H16_A1012(h16_A1012)
RME: Rmet_0888
CGD: CR3_0823
BMA: BMA0599
BMAL: DM55_1973
BMAE: DM78_1191
BMAQ: DM76_1948
BMAI: DM57_2614
BMAF: DM51_385
BMAZ: BM44_1462
BMAB: BM45_1025
BPS: BPSL2382
BPSE: BDL_3108
BPSM: BBQ_930
BPSU: BBN_1057
BPSD: BBX_1509
BPK: BBK_2574
BPSH: DR55_2139
BPSA: BBU_3179
BPSO: X996_1783
BUT: X994_55
BTE: BTH_I1784
BTQ: BTQ_2131
BTJ: BTJ_180
BTZ: BTL_1475
BTD: BTI_1253
BTV: BTHA_1579
BTHE: BTN_3300
BTHM: BTRA_1701
BTHA: DR62_70
BTHL: BG87_1688
BOK: DM82_1996
BOC: BG90_2716
BCT: GEM_0728
BUB: BW23_2182
BGO: BM43_3359
BUL: BW21_1402
PNU: Pnuc_0032
PNE: Pnec_0028
HYF: DTO96_101909(desA)
TEA: KUI_0809
TEG: KUK_0647
TAS: TASI_0874
TAT: KUM_0089
AMIM: MIM_c19480(desA)
BPSI: IX83_04375
POL: Bpro_4293
LIM: L103DPR2_00206(desA)
LIH: L63ED372_00157(desA)
HPSE: HPF_05925(desA)
CBAA: SRAA_1604
CBAB: SMCB_1509
MPT: Mpe_A3596
CFU: CFU_1080(desA)
CARE: LT85_4631
BPRC: D521_0028
DEU: DBW_2083(des)
DFL: DFE_2500
PPRF: DPRO_1331(des)
DALK: DSCA_47420(des)
CCX: COCOR_03682(des)
HOH: Hoch_5461
MLO: mlr3223
MHUA: MCHK_4718
AMIH: CO731_01147(des)
PLA: Plav_3282
RBS: RHODOSMS8_02108(des)
SME: SMa0130
SMI: BN406_05015(des)
SMER: DU99_25100
SMD: Smed_5160
SFD: USDA257_c29680(des)
SIX: BSY16_465
SAME: SAMCFNEI73_pC0188(des)
ATF: Ach5_35920(desA)
RLE: RL1400
RLG: Rleg_1030
RHT: NT26_1947(des)
BJA: blr2247
BBT: BBta_3000
BRS: S23_57700
BRAD: BF49_2678
RPA: RPA1603
RPE: RPE_1624
RPT: Rpal_1793
VGO: GJW-30_1_04435(des)
SNO: Snov_0783
MDI: METDI3756
MEX: Mext_2986
MCH: Mchl_3212
META: Y590_14475
MAQU: Maq22A_c18435(desA)
HDN: Hden_3148
PLEO: OHA_1_02879(des)
MMED: Mame_01682(des)
HDI: HDIA_4009(des)
RBM: TEF_21325
SIL: SPO2327
RUT: FIU92_01705(des1) FIU92_09815(des2)
JAN: Jann_2995
RDE: RD1_0987
RLI: RLO149_c001940(des)
OTM: OSB_19200(des)
MALG: MALG_02005
LABT: FIU93_29745(des)
SPSE: SULPSESMR1_02345(des)
RID: RIdsm_02229(des_1) RIdsm_05013(des_2)
ROH: FIU89_07830(des)
AHT: ANTHELSMS3_03872(des)
ROT: FIV09_02915(des)
HBA: Hbal_1590
ELI: ELI_09590
ERF: FIU90_15490(des)
SHUM: STHU_34550
AZL: AZL_011320(desA)
TMO: TMO_c0486
AFR: AFE_1964
ACU: Atc_1645
ATX: GCD22_01405(fad6)
BSU: BSU19180(des)
BSL: A7A1_2788
BSH: BSU6051_19180(des)
BSUT: BSUB_02064(des)
BSUL: BSUA_02064(des)
BSUS: Q433_11415
BSS: BSUW23_02535(dfaD) BSUW23_10270(des)
BSO: BSNT_08479(des)
BSQ: B657_19180(des)
BSX: C663_0487(FAD6) C663_1968(yocE)
BLI: BL02106(des) BL02692
BLD: BLi00576(des1) BLi02807(des2)
BLH: BaLi_c06010(des1) BaLi_c29020(des2)
BAQ: BACAU_3701(des)
BYA: BANAU_0451(des1) BANAU_3870(des3)
BAML: BAM5036_3606(des)
BAMA: RBAU_0506 RBAU_3814(des)
BAMN: BASU_0497 BASU_3596(des)
BAMB: BAPNAU_0463(desA) BAPNAU_3877(des)
BAMT: AJ82_20790
BMP: NG74_00509(des_1) NG74_03861(des_2)
BAO: BAMF_3796(des)
BAZ: BAMTA208_20070(des)
BQL: LL3_04119(des)
BXH: BAXH7_04117(des)
BQY: MUS_0493 MUS_4378(des)
BAMI: KSO_000655
BAMF: U722_19635
BAN: BA_3000
BAR: GBAA_3000
BAT: BAS2788
BANT: A16_58815
BANR: A16R_59655
BANS: BAPAT_2877
BANV: DJ46_1761
BCE: BC2983
BCA: BCE_3036
BCQ: BCQ_2816
BCX: BCA_3070
BNC: BCN_2840
BCF: bcf_14645
BCER: BCK_19910
BTL: BALH_2685
BTT: HD73_2987
BTHI: BTK_14805
BTM: MC28_2187
BTG: BTB_c31130(des)
BTI: BTG_04415
BTW: BF38_4153
BWW: bwei_2028(des1)
BMYO: BG05_3030
BPU: BPUM_2588
BPUM: BW16_13955
BPUS: UP12_13240
BMQ: BMQ_1492(des)
BMD: BMD_1474(des)
BJS: MY9_2098
BMET: BMMGA3_08030(des)
BACW: QR42_13050
BACP: SB24_10325
BACO: OXB_0780
BACL: BS34A_21270(des)
BALM: BsLM_2036
BEO: BEH_22575
BBEV: BBEV_0053(des)
BCL: ABC0734(des)
BPF: BpOF4_10025(desA)
BKW: BkAM31D_02770(des)
OIH: OB2852
LSP: Bsph_0178
HHD: HBHAL_5012(des)
HLI: HLI_12850
PSYO: PB01_00630
BSE: Bsel_3286
SXO: SXYL_02322(desA)
MCL: MCCL_0154
MCAK: MCCS_02710(des)
ESI: Exig_2596
EAN: Eab7_2435
BBE: BBR47_07920(des)
PPY: PPE_00885
PPM: PPSC2_04595(des)
PPO: PPM_0861(des)
PPOL: X809_04210
PPQ: PPSQR21_009170(desA)
PPOY: RE92_07370
PMS: KNP414_07230(des)
PMW: B2K_34290
PRI: PRIO_3035(des)
PSWU: SY83_01890
SIV: SSIL_0449
JEO: JMA_06180
KUR: ASO14_349
STRM: M444_36405
SALJ: SMD11_0945
CWO: Cwoe_3764
SYN: slr1350(desA)
SYZ: MYO_116090(desA)
SYY: SYNGTS_1594(desA)
SYT: SYNGTI_1594(desA)
SYS: SYNPCCN_1593(desA)
SYQ: SYNPCCP_1593(desA)
SYJ: D082_29510(desA)
SYW: SYNW1696
SYG: sync_0396
SYP: SYNPCC7002_A2756(desA)
SYNR: KR49_12985
SYH: Syncc8109_1775(desA)
SYNW: SynWH8103_01945(desC)
PSER: ABRG53_1847(desA)
PMA: Pro_1208(desA)
PMM: PMM1378(des)
PMT: PMT_0797
PMH: P9215_16141(desA)
PRC: EW14_1688
AMR: AM1_4659
MAR: MAE_53570(desA)
MPK: VL20_1481
MVZ: myaer102_26250(desA)
CYT: cce_3178(desA)
TER: Tery_0142
GVI: gll0171 gvip364(desA)
GLJ: GKIL_2247(desA) GKIL_3937
ANA: all1598(desA)
NSH: GXM_01595
AVA: Ava_4211
NAZ: Aazo_3555
CALH: IJ00_05010
DOU: BMF77_00180(desA_1) BMF77_00980(des)
CTHE: Chro_0114
CEO: ETSB_0346(desA)
CER: RGRSB_0303(desA)
ATM: ANT_25280
TTH: TT_C0582
TTJ: TTHA0948
TAQ: TO73_0845
CAA: Caka_1354
MIN: Minf_0111(desA)
MKC: kam1_131
PLH: VT85_15130(desA)
LIL: LA_0502
LIE: LIF_A0419
LIC: LIC_13053(desA)
LIS: LIL_13164(desA2)
GBA: J421_1530
DORI: FH5T_11125
CPI: Cpin_2594
KOS: KORDIASMS9_02140(des)
KAN: IMCC3317_39030(des)
BPI: BPLAN_488
BMM: MADAR_465
CABY: Cabys_2212
 » show all
Reference
1  [PMID:11607123]
  Authors
Schmidt H, Heinz E
  Title
Desaturation of oleoyl groups in envelope membranes from spinach chloroplasts.
  Journal
Proc Natl Acad Sci U S A 87:9477-80 (1990)
DOI:10.1073/pnas.87.23.9477
Reference
2  [PMID:16667689]
  Authors
Schmidt H, Heinz E
  Title
Involvement of ferredoxin in desaturation of lipid-bound oleate in chloroplasts.
  Journal
Plant Physiol 94:214-20 (1990)
DOI:10.1104/pp.94.1.214
Reference
3  [PMID:8066133]
  Authors
Hitz WD, Carlson TJ, Booth JR Jr, Kinney AJ, Stecca KL, Yadav NS
  Title
Cloning of a higher-plant plastid omega-6 fatty acid desaturase cDNA and its expression in a cyanobacterium.
  Journal
Plant Physiol 105:635-41 (1994)
DOI:10.1104/pp.105.2.635
  Sequence
[bna:106434448] [gmx:547807]
Reference
4  [PMID:7846158]
  Authors
Falcone DL, Gibson S, Lemieux B, Somerville C
  Title
Identification of a gene that complements an Arabidopsis mutant deficient in chloroplast omega 6 desaturase activity.
  Journal
Plant Physiol 106:1453-9 (1994)
DOI:10.1104/pp.106.4.1453
  Sequence
[ath:AT4G30950]
Reference
5  [PMID:7948918]
  Authors
Schmidt H, Dresselhaus T, Buck F, Heinz E
  Title
Purification and PCR-based cDNA cloning of a plastidial n-6 desaturase.
  Journal
Plant Mol Biol 26:631-42 (1994)
DOI:10.1007/bf00013749
Other DBs
ExplorEnz - The Enzyme Database: 1.14.19.23
IUBMB Enzyme Nomenclature: 1.14.19.23
ExPASy - ENZYME nomenclature database: 1.14.19.23
BRENDA, the Enzyme Database: 1.14.19.23

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