Entry
Name
acyl-lipid (n+3)-(Z)-desaturase (ferredoxin);
acyl-lipid omega6-desaturase (ferredoxin);
oleate desaturase (ambiguous);
linoleate synthase (ambiguous);
oleoyl-CoA desaturase (ambiguous);
oleoylphosphatidylcholine desaturase (ambiguous);
phosphatidylcholine desaturase (ambiguous);
FAD6 (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
BRITE hierarchy
Sysname
oleoyl-[glycerolipid],ferredoxin:oxygen oxidoreductase (12,13 cis-dehydrogenating)
Reaction(IUBMB)
an oleoyl-[glycerolipid] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+ = a linoleoyl-[glycerolipid] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
Substrate
oleoyl-[glycerolipid];
reduced ferredoxin [iron-sulfur] cluster [CPD:
C00138 ];
O2 [CPD:
C00007 ];
H+ [CPD:
C00080 ]
Product
linoleoyl-[glycerolipid] [CPD:
C21529 ];
oxidized ferredoxin [iron-sulfur] cluster [CPD:
C00139 ];
H2O [CPD:
C00001 ]
Comment
This plastidial enzyme is able to insert a cis double bond in monounsaturated fatty acids incorporated into glycerolipids. The enzyme introduces the new bond at a position 3 carbons away from the existing double bond, towards the methyl end of the fatty acid. The native substrates are oleoyl (18:1 Delta9) and (Z)-hexadec-7-enoyl (16:1 Delta7) groups attached to either position of the glycerol backbone in glycerolipids, resulting in the introduction of the second double bond at positions 12 and 10, respectively This prompted the suggestion that this is an omega6 desaturase. However, when acting on palmitoleoyl groups(16:1 Delta9), the enzyme introduces the second double bond at position 12 (omega4), indicating it is an (n+3) desaturase [3]. cf. EC
1.14.19.34 , acyl-lipid (9+3)-(E)-desaturase.
History
EC 1.14.19.23 created 2015
Orthology
K10255 acyl-lipid omega-6 desaturase (Delta-12 desaturase)
Genes
CSAT : 104717096 104727652 104730201
BNA : 106359134 106376162 106424191 106434448
RSZ : 108819010 108825849 108831671 108834911
GMX : 100798793(FAD6.2) 547807(FAD6.1)
PSAT : 127097830 127138422
LJA : Lj4g3v1983380.1(Lj4g3v1983380.1)
LANG : 109327644 109354996
PCIN : 129295355 129309392
CMAX : 111465334 111485774
CMOS : 111429776 111436860
CPEP : 111779726 111792327
MESC : 110607377 110617171
SSPL : 121749743 121750630
ECAD : 122596092 122597594
MOF : 131158874 131158876 131158877 131158880
PSOM : 113313245 113325422 113332079 113347290
TAES : 100125744 123052234 123188165
PVIR : 120677325 120712480
SMO : SELMODRAFT_111378 SELMODRAFT_111460 SELMODRAFT_122097 SELMODRAFT_122116 SELMODRAFT_13166 SELMODRAFT_153911
PPP : 112289446 112292746 112293087
CRE : CHLRE_06g288650v5 CHLRE_13g590500v5
MNG : MNEG_14165 MNEG_3236
PPRC : PFLCHA0_c02700(desA)
REH : H16_A1012(h16_A1012)
BVI : Bcep1808_2819 Bcep1808_4012
BSTG : WT74_12815 WT74_14380
LIM : L103DPR2_00206(desA)
LIH : L63ED372_00157(desA)
BBAG : E1O_11130 E1O_21310
RBS : RHODOSMS8_02108(des)
SAME : SAMCFNEI73_pC0188(des)
RHL : LPU83_3623 LPU83_pLPU83d0685(des)
NHA : Nham_1170 Nham_2541 Nham_4601
MAQU : Maq22A_c18435(desA)
RHZ : RHPLAN_52980 RHPLAN_57690
TSV : DSM104635_02735(des)
RUA : D1823_03105 D1823_11775
RUT : FIU92_01705(des1) FIU92_09815(des2)
RCON : K3740_02400 K3740_10790 K3740_20885 K3740_21470
PGA : PGA1_c17310 PGA1_c18980
PGL : PGA2_c17110 PGA2_c18060
PHP : PhaeoP97_01734 PhaeoP97_01934
PPIC : PhaeoP14_01629 PhaeoP14_01746
PHQ : D1820_04610 D1820_05085
OCT : FTO60_06850 FTO60_10460
LMD : METH_07930 METH_09105
LEJ : ETW24_10280 ETW24_10860
LAQU : R2C4_09190 R2C4_09825
LEV : ETW23_09915 ETW23_10730
LCAE : K3721_07365 K3721_08010 K3721_18905
CEH : CEW89_01900 CEW89_01940
SULI : C1J05_13120 C1J05_14115
SINL : DSM14862_00511(des)
SPSE : SULPSESMR1_02345(des)
RMM : ROSMUCSMR3_00803(des) ROSMUCSMR3_02984(des)
ROK : RAK1035_1883 RAK1035_3675
RID : RIdsm_02229(des_1) RIdsm_05013(des_2)
AHT : ANTHELSMS3_03872(des)
TGL : HFZ77_01450 HFZ77_06135
PZH : CX676_20805 CX676_21940
PLIA : E4191_21320 E4191_22975
RMB : K529_004305 K529_008420
LVS : LOKVESSMR4R_03368(des)
SEDI : EBB79_06385 EBB79_15255
PAED : G5B38_04870 G5B38_19825
GCE : KYE46_07445 KYE46_13505
LCK : HN018_18665 HN018_20455
HFL : PUV54_05450 PUV54_06910
ACZ : Acaty_c1394 Acaty_c1601
PPOR : JCM14722_24100(des)
MFB : MFUL124B02_30240 MFUL124B02_36860
MFU : LILAB_25445 LILAB_34815
MMAS : MYMAC_003436 MYMAC_005263
MRM : A7982_05413 A7982_13471
BSY : I653_02505 I653_09920
BSX : C663_0487(FAD6) C663_1968(yocE)
BSS : BSUW23_02535(dfaD) BSUW23_10270(des)
BIT : BIS30_00135 BIS30_13065
BLI : BL02106(des) BL02692
BLD : BLi00576(des1) BLi02807(des2)
BLH : BaLi_c06010(des1) BaLi_c29020(des2)
BAY : RBAM_005380 RBAM_036870
BYA : BANAU_0451(des1) BANAU_3870(des3)
BAMP : B938_02465 B938_18905
BQY : MUS_0493 MUS_4378(des)
BAMA : RBAU_0506 RBAU_3814(des)
BAMN : BASU_0497 BASU_3596(des)
BAMB : BAPNAU_0463(desA) BAPNAU_3877(des)
BAMY : V529_04870 V529_39540(des)
BMP : NG74_00509(des_1) NG74_03861(des_2)
BAMC : U471_05040 U471_38460
BAE : BATR1942_00140 BATR1942_08255
BSON : S101395_01720(fad6) S101395_04432
BHT : DIC78_07345 DIC78_21005
BMOJ : HC660_05680(desE) HC660_19850
BTEQ : G4P54_02810 G4P54_11020
BSTR : QI003_10375(desE) QI003_13805(desE)
BIF : N288_04805 N288_13375
BFD : NCTC4823_03211(desA)
BBAD : K7T73_08310 K7T73_10055
BCAB : EFK13_02960 EFK13_10635(desE)
BRY : M0696_02805 M0696_10325(desE)
BACB : OY17_02110 OY17_05290
BACY : QF06_01445 QF06_08705
BACA : FAY30_07900 FAY30_14280
BMEG : BG04_3784 BG04_4087
HNZ : P9989_09020 P9989_20960
BSJ : UP17_20795 UP17_23350
PBUT : DTO10_07235 DTO10_09815
PASA : BAOM_2837 BAOM_4551
PPSR : I6J18_05325 I6J18_16635
PFRI : L8956_21605 L8956_24845
NMK : CHR53_08520 CHR53_26385
NCM : QNK12_07785 QNK12_17880
BLEN : NCTC4824_02221(des)
PPQ : PPSQR21_009170(desA)
PALB : EJC50_18180 EJC50_25370
AFX : JZ786_07725 JZ786_08125
AACO : K1I37_15815 K1I37_16565
ACUR : JZ785_00660 JZ785_13040
ADAU : NZD86_02985 NZD86_12995
AFAS : NZD89_05130 NZD89_09105 NZD89_22755 NZD89_24310
SGF : HEP81_00049(desA_1) HEP81_07829(desA_2)
SBAE : DSM104329_02773(des)
SYP : SYNPCC7002_A2756(desA)
SYH : Syncc8109_1775(desA)
SYNW : SynWH8103_01945(desC)
SYV : AWQ23_13715 AWQ23_15550
SYNY : BM449_00735 BM449_11890
CMP : Cha6605_3200 Cha6605_4757 Cha6605_6102
LEP : Lepto7376_0029 Lepto7376_0786
LBO : LBWT_13260 LBWT_4850
MVZ : myaer102_26250(desA)
CWA : CwatDRAFT_3226 CwatDRAFT_5150
CYP : PCC8801_2805 PCC8801_3498
CYH : Cyan8802_2618 Cyan8802_3295
CYN : Cyan7425_2878 Cyan7425_2879 Cyan7425_4259
ARP : NIES39_A06590(desA) NIES39_A07580
PAGH : NIES204_04070 NIES204_13690(desA)
PPSU : NO713_01858(des) NO713_04820(desA)
MVAG : D0A34_03380 D0A34_05440
GVI : gll0171 glr2623(desA)
GLJ : GKIL_2247(desA) GKIL_3937
ANB : ANA_C20203 ANA_C20444
AWA : AA650_16765 AA650_25570
NSPH : BDGGKGIB_02540(desA_2)
DOU : BMF77_00180(desA_1) BMF77_00980(des)
DFS : HGD76_14235 HGD76_17200
AFLO : HEQ12_18920 HEQ12_19075
LKB : LPTSP3_g23720(desA_2)
KOS : KORDIASMS9_02140(des)
» show all
Taxonomy
Reference
Authors
Schmidt H, Heinz E
Title
Desaturation of oleoyl groups in envelope membranes from spinach chloroplasts.
Journal
Reference
Authors
Schmidt H, Heinz E
Title
Involvement of ferredoxin in desaturation of lipid-bound oleate in chloroplasts.
Journal
Reference
Authors
Hitz WD, Carlson TJ, Booth JR Jr, Kinney AJ, Stecca KL, Yadav NS
Title
Cloning of a higher-plant plastid omega-6 fatty acid desaturase cDNA and its expression in a cyanobacterium.
Journal
Sequence
Reference
Authors
Falcone DL, Gibson S, Lemieux B, Somerville C
Title
Identification of a gene that complements an Arabidopsis mutant deficient in chloroplast omega 6 desaturase activity.
Journal
Sequence
Reference
Authors
Schmidt H, Dresselhaus T, Buck F, Heinz E
Title
Purification and PCR-based cDNA cloning of a plastidial n-6 desaturase.
Journal
Other DBs