Entry
Name
acyl-lipid omega-3 desaturase (cytochrome b5);
FAD3
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
BRITE hierarchy
Sysname
(9Z,12Z)-octadeca-9,12-dienoyl-[glycerolipid],ferrocytochrome b5:oxygen oxidoreductase (15,16 cis-dehydrogenating)
Reaction(IUBMB)
a linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+ = an alpha-linolenoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O [RN:
R12416 ]
Reaction(KEGG)
Substrate
Product
alpha-linolenoyl-[glycerolipid] [CPD:
C21531 ];
ferricytochrome b5 [CPD:
C00996 ];
H2O [CPD:
C00001 ]
Comment
This microsomal enzyme introduces a cis double bond three carbons away from the methyl end of a fatty acid incorporated into a glycerolipid. The distance from the carboxylic acid end of the molecule does not have an effect. The plant enzyme acts on carbon 15 of linoleoyl groups incorporated into both the sn-1 and sn-2 positions of the glycerol backbone of phosphatidylcholine and other phospholipids, converting them into alpha-linolenoyl groups. The enzyme from the fungus Mortierella alpina acts on gamma-linolenoyl and arachidonoyl groups, converting them into stearidonoyl and icosapentaenoyl groups, respectively [3]. cf. EC
1.14.19.35 , sn-2 acyl-lipid omega-3 desaturase (ferredoxin).
History
EC 1.14.19.25 created 2015
Orthology
K10257 acyl-lipid omega-3 desaturase
Genes
CEL : CELE_W02A2.1(fat-2) CELE_Y67H2A.8(fat-1)
CBR : CBG_01790(Cbr-fat-2) CBG_01791(Cbr-fat-1)
CRQ : GCK72_014531 GCK72_014532
LGI : LOTGIDRAFT_116269 LOTGIDRAFT_74451
LJP : 135464652 135479298 135479509
ATH : AT2G29980(FAD3) AT3G11170(FAD7) AT5G05580(FAD8)
ALY : 9307241 9315309 9320901
CRB : 17881387 17889809 17891716
CSAT : 104700502 104708554 104734563 104745318 104749896 104764868 104765502 104768754 104786676
EUS : EUTSA_v10013625mg EUTSA_v10016786mg EUTSA_v10020755mg
BRP : 103850591 103857278 103859381 103864998 103867994 103870371
BNA : 106353041 106377255 106380841 106387896 106418558 106422764 106429840 106439274 106446979 106452545 111197728 125584238 125585240 125595996
BOE : 106295700 106314490 106327053 106328829 106335120 106336554 106336680
RSZ : 108811531 108837004 108843142 108844225 108848571 108859156 108862810
THJ : 104800741 104816198 104816543 104826849
CPAP : 110808059 110810025 110820958
CIC : CICLE_v10011691mg CICLE_v10028941mg
PVY : 116115152 116121787 116122546 116129441 116129513
MINC : 123192198 123201556 123209054 123209055 123228271
TCC : 18585841 18590479 18597527
GRA : 105776289 105780050 105786551 105791965 105799158
GHI : 107889065 107891556 107891614 107899542 107931808 107940880 107948941 107956656 107958053 121207887
GAB : 108454561 108457033 108461005 108464318 108466542 108480912
HSYR : 120116026 120116384 120138616 120142041 120144038 120168127 120176495 120200614 120208705 120209961
DZI : 111285613 111293917 111296353 111304348 111310113 111312056
GMX : 100037456(FAD3B) 100217335(FAD8-1) 100305351(FAD7-1) 100499626(FAD7-2) 100799517(FAD8.2) 547470(FAD3C) 547715(FAD3A) 732540(FAD3-2b)
GSJ : 114374933 114383326 114394696 114395027 114396629 114406122 114409044 114416093 114419078
PVU : 137812596 137823485 137823720 137831477
VRA : 106759364 106765282 106774181 106775023
VAR : 108329591 108337050 108341784 108347618
VUN : 114167372 114185384 114186845 114193336
VUM : 124823089 124824366 124827010 124829301
CCAJ : 109787870 109813625 109815393 109816410
APRC : 113846079 113849137 113851549 113863868
MTR : 11411563 11415576 25490906 25498767
TPRA : 123889397 123892471 123914473 123922449 123924861
CAM : 101502461 101504225 101513464 101515266
PSAT : 127084563 127107471 127118542 127127449
VVO : 131601323 131609384 131635342 131637230 131639719 131662199
LJA : 130723719 130733986 130748795
ADU : 107458340 107475149 107484970 107491190
AIP : 107609767 107625758 107639562 107644755
AHF : 112702815 112720001 112728003 112743658 112750362 112766655 112792318 112797044
LANG : 109330327 109338970 109349642 109350266 109352644 109360270 109361525
PCIN : 129289510 129290769 129298447
QSA : O6P43_014054 O6P43_016593 O6P43_020355 O6P43_026237
FVE : 101306021 101306861 101310644 101312263
RCN : 112165678 112182995 112193656
PPER : 18772228 18773198 18774962 18785351
PMUM : 103320961 103320972 103333043 103341784
PAVI : 110749762 110758660 110758749 110770476
PDUL : 117613027 117617094 117631111 117631112 117632464
MDM : 103419381 103420048 103430794 103432837 103447381 103447641
MSYL : 126590692 126590694 126592394 126603049 126616550 126617350 126617712 126629706
PXB : 103930542 103931220 103931253 103936863 103938921 103958619 103966319
ZJU : 107404190 107410778 107430888
CSAV : 115697681 115705594 115720710
CSV : 101209295 101223135(FAD7)
CMAX : 111469542 111489597 111491157 111492652
CMOS : 111447744 111452212 111458375 111464727
CPEP : 111778662 111782701 111787876 111806684
RCU : 8265360 8286343 8286466
MEAN : 126653627 126664767 126682098
JCU : 105630235(FAD3) 105646096(FAD) 105646580
HBR : 110648982 110654511 110654544 110657237 110665331 110666427
MESC : 110613473 110619516 110619524 110620027 110624941
POP : 7466077 7468059 7478523 7490323 7494502
PEU : 105111171 105121928 105133573 105139146 105142210
PALZ : 118033024 118037588 118043346 118048506 118055844
PNZ : 133677731 133684351 133689657 133696046 133704228
JRE : 108989903 108989905 109002248 109007160 109015144
CILL : 122276706 122290752 122293355 122301503
CAVE : 132161849 132163977 132186916
QSU : 112011944 112030173 112031015 136070956
TWL : 119998266 120001334 120012033
VVI : 100243349 100247097 100251798 100260414
VRI : 117915999 117917133 117921171 117928747
SLY : 100134915 544203(Fad7)
SPEN : 107021758 107023114 107023926
SOT : 102596735 102597287 102597672
SSTN : 125872918 125872941
SDUL : 129881617 129899532 129901404 129901470
CANN : 107873310 107873419 107873420
LBB : 132605801 132608932 132631607
NTA : 107763446(FAD3) 107777511 107783156(NtFAD7) 107786526(NtFAD3-2) 107804927 107807128 107832399
NSY : 104237848 104243629 104243898 104248927
NTO : 104090878 104112653 104114074
NAU : 109213818 109214388 109238135
INI : 109147953 109164374 109186614
ITR : 116000429 116013334 116014945
SIND : 105160895 105163949 105167042
OEU : 111373879 111401332 111403039 111403247
EGT : 105960105 105969857 105977522
SSPL : 121748592 121762807 121763363 121774873 121777347 121779911 121782532 121786158 121791991
SMIL : 130992039 131003346 131005695
SHIS : 125201775 125202091 125207882 125215068
APAN : 127244856 127251442
HAN : 110868642 110868972 110872089 110872729 110878910 110885030 110907224 110922364
ECAD : 122582349 122594010 122600878 122602668 122606927
LSV : 111882696 111893204 111901267 111912570 111919702
CCAV : 112506819 112512083 112514077 112518049 112526060
DCR : 108192445 108206807 108209044 108210458 108219754
CSIN : 114283960 114287210 114292016 114315720 114318519
RVL : 131314300 131320560 131323256 131323270 131333756
AEW : 130761839 130764381 130770938 130773102 130774758 130774762 130783024 130791525 130794728 130795216
CQI : 110699288 110699289 110733453 110733454
ATRI : 130803064 130803067 130803850
MOF : 131143500 131147647 131149084 131149085
MING : 122085160 122089304
TSS : 122651879 122669968 122670968
PSOM : 113309945 113310323 113320193 113338748
OSA : 4332500 4344389 4349544 4351253
OBR : 102707070 102715194 102720167 102720386
OGL : 127754012 127756827 127768014 127779695
BDI : 100824939 100831377 100840287
ATS : 109748184 109753536 109765018 109778723
TDC : 119285393 119286162 119290627 119295655 119301018 119308532 119353119 119362211
TAES : 123051381 123085571 123086342 123091705 123092458 123096668 123097784 123103230 123120533 123187329 543187 543189
TUA : 125508245 125508814 125535517 125552086
LPER : 127294720 127295843 127300174 127331350
LRD : 124652208 124652514 124678689 124699202 124702263
SBI : 8055482 8072903 8079958 8084033 8084992
ZMA : 100281245 100283566 103644592 542222
MFLO : 136481330 136486043 136504942 136509143 136533113 136535876 136542909 136546866
SITA : 101771745 101776425 101785330
SVS : 117834208 117839178 117863610
PVIR : 120643370 120652727 120686119 120689735 120694048 120696494
PHAI : 112875540 112893600
PDA : 103706691 103709505 103718325
EGU : 105044596 105046171 105057100
MUS : 103984918 103986078 103995423 135618607 135622503 135624204 135626921
ZOF : 121983175 121987737 121989428 121998376 122000948 122005229 122020419 122023714 122035993 122036024
DCT : 110105024 110110723 110110724
PEQ : 110023217 110023647 110023671
MSIN : 131223588 131253315
ACAK : 137936078 137965927
SMO : SELMODRAFT_103593 SELMODRAFT_109519
PPP : 112277833 112279588 112290291 112290971 112294054 112294506
MNG : MNEG_12033 MNEG_15487 MNEG_9648
PTI : PHATRDRAFT_41570(PTD15)
SYP : SYNPCC7002_A0159(desB)
SYNN : NIES970_02080(desB)
MVZ : myaer102_09850(desB)
PRUN : PCC7821_04820(FAD7)
NCF : ANSO36C_61810(desA_1)
NSPH : BDGGKGIB_02539(desA_1)
LKB : LPTSP3_g23450(desA_1)
» show all
Taxonomy
Reference
Authors
Browse J, McConn M, James D Jr, Miquel M
Title
Mutants of Arabidopsis deficient in the synthesis of alpha-linolenate. Biochemical and genetic characterization of the endoplasmic reticulum linoleoyl desaturase.
Journal
J Biol Chem 268:16345-51 (1993)
Sequence
Reference
Authors
Arondel V, Lemieux B, Hwang I, Gibson S, Goodman HM, Somerville CR
Title
Map-based cloning of a gene controlling omega-3 fatty acid desaturation in Arabidopsis.
Journal
Sequence
Reference
Authors
Sakuradani E, Abe T, Iguchi K, Shimizu S
Title
A novel fungal omega3-desaturase with wide substrate specificity from arachidonic acid-producing Mortierella alpina 1S-4.
Journal
Other DBs