KEGG   ENZYME: 1.14.19.33
Entry
EC 1.14.19.33               Enzyme                                 

Name
Delta12 acyl-lipid conjugase (11E,13E-forming);
fatty acid Delta12-conjugase (ambiguous);
FADX (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
Sysname
Delta12 acyl-lipid,ferrocytochrome-b5:oxygen 11,14 allylic oxidase (11E,13E-forming)
Reaction(IUBMB)
(1) a linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+ = an alpha-eleostearoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O;
(2) a gamma-linolenoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+ = an alpha-parinaroyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
Substrate
linoleoyl-[glycerolipid] [CPD:C21529];
ferrocytochrome b5 [CPD:C00999];
O2 [CPD:C00007];
H+ [CPD:C00080];
gamma-linolenoyl-[glycerolipid]
Product
alpha-eleostearoyl-[glycerolipid];
ferricytochrome b5 [CPD:C00996];
H2O [CPD:C00001];
alpha-parinaroyl-[glycerolipid]
Comment
The enzyme, characterized from the plants Impatiens balsamina, Momordica charantia (bitter gourd) and Vernicia fordii (tung tree), converts a single cis double bond at carbon 12 to two conjugated trans bonds at positions 11 and 13. The enzyme from Vernicia fordii can also act as a 12(E) desaturase when acting on the monounsaturated fatty acids oleate and palmitoleate. cf. EC 1.14.19.16, linoleoyl-lipid Delta12 conjugase (11E,13Z-forming).
History
EC 1.14.19.33 created 2015
Orthology
K21736  Delta12 acyl-lipid conjugase (11E,13E-forming) / acyl-lipid (9+3)-(E)-desaturase
Genes
CIT: 102611163
CIC: CICLE_v10015614mg
JCU: 105650891
HBR: 110638358
VVI: 100258789
Reference
1  [PMID:10536026]
  Authors
Cahoon EB, Carlson TJ, Ripp KG, Schweiger BJ, Cook GA, Hall SE, Kinney AJ
  Title
Biosynthetic origin of conjugated double bonds: production of fatty acid components of high-value drying oils in transgenic soybean embryos.
  Journal
Proc Natl Acad Sci U S A 96:12935-40 (1999)
DOI:10.1073/pnas.96.22.12935
Reference
2  [PMID:12481086]
  Authors
Dyer JM, Chapital DC, Kuan JC, Mullen RT, Turner C, McKeon TA, Pepperman AB
  Title
Molecular analysis of a bifunctional fatty acid conjugase/desaturase from tung. Implications for the evolution of plant fatty acid diversity.
  Journal
Plant Physiol 130:2027-38 (2002)
DOI:10.1104/pp.102.010835
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.19.33
IUBMB Enzyme Nomenclature: 1.14.19.33
ExPASy - ENZYME nomenclature database: 1.14.19.33
BRENDA, the Enzyme Database: 1.14.19.33

DBGET integrated database retrieval system