KEGG   ENZYME: 1.14.19.34Help
Entry
EC 1.14.19.34               Enzyme                                 

Name
acyl-lipid (9+3)-(E)-desaturase;
acyl-lipid 12-(E)-desaturase;
DsFAD2-1;
FADX
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
BRITE hierarchy
Sysname
Delta9 acyl-lipid,ferrocytochrome b5:oxygen oxidoreductase (12,13 trans-dehydrogenating)
Reaction(IUBMB)
(1) an oleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+ = a (9Z,12E)-octadeca-9,12-dienoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O;
(2) a palmitoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+ = a (9Z,12E)-hexadeca-9,12-dienoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
Substrate
oleoyl-[glycerolipid];
ferrocytochrome b5 [CPD:C00999];
O2 [CPD:C00007];
H+ [CPD:C00080];
palmitoleoyl-[glycerolipid]
Product
(9Z,12E)-octadeca-9,12-dienoyl-[glycerolipid];
ferricytochrome b5 [CPD:C00996];
H2O [CPD:C00001];
(9Z,12E)-hexadeca-9,12-dienoyl-[glycerolipid]
Comment
The enzymes from the plants Dimorphotheca sinuata (African daisy) and Vernicia fordii (tung oil tree) insert a trans double bond in position C-12 of oleate and palmitoleate incorporated into glycerolipids. The enzyme introduces the new double bond at a position three carbons away from an existing double bond at position 9, towards the methyl end of the fatty acid. The enzyme from tung oil tree also possesses the activity of EC 1.14.19.33, Delta12 acyl-lipid conjugase.
History
EC 1.14.19.34 created 2015
Orthology
K21736  Delta12 acyl-lipid conjugase (11E,13E-forming) / acyl-lipid (9+3)-(E)-desaturase
Genes
CIT: 102611163
CIC: CICLE_v10015614mg
JCU: 105650891
HBR: 110638358
VVI: 100258789
Taxonomy
Reference
1  [PMID:12481086]
  Authors
Dyer JM, Chapital DC, Kuan JC, Mullen RT, Turner C, McKeon TA, Pepperman AB
  Title
Molecular analysis of a bifunctional fatty acid conjugase/desaturase from tung. Implications for the evolution of plant fatty acid diversity.
  Journal
Plant Physiol 130:2027-38 (2002)
DOI:10.1104/pp.102.010835
  Sequence
Reference
2  [PMID:14718523]
  Authors
Cahoon EB, Kinney AJ
  Title
Dimorphecolic acid is synthesized by the coordinate activities of two divergent Delta12-oleic acid desaturases.
  Journal
J Biol Chem 279:12495-502 (2004)
DOI:10.1074/jbc.M314329200
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.19.34
IUBMB Enzyme Nomenclature: 1.14.19.34
ExPASy - ENZYME nomenclature database: 1.14.19.34
BRENDA, the Enzyme Database: 1.14.19.34

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