KEGG   ENZYME: 1.14.19.35Help
Entry
EC 1.14.19.35               Enzyme                                 

Name
sn-2 acyl-lipid omega-3 desaturase (ferredoxin);
FAD7;
FAD8
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
BRITE hierarchy
Sysname
(7Z,10Z)-hexadeca-7,10-dienoyl-[glycerolipid],ferredoxin:oxygen oxidoreductase (13,14 cis-dehydrogenating)
Reaction(IUBMB)
(1) a (7Z,10Z)-hexadeca-7,10-dienoyl-[glycerolipid] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+ = a (7Z,10Z,13Z)-hexadeca-7,10,13-trienoyl-[glycerolipid] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O;
(2) a linoleoyl-[glycerolipid] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+ = an alpha-linolenoyl-[glycerolipid] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
Substrate
(7Z,10Z)-hexadeca-7,10-dienoyl-[glycerolipid];
reduced ferredoxin [iron-sulfur] cluster [CPD:C00138];
O2 [CPD:C00007];
H+ [CPD:C00080];
linoleoyl-[glycerolipid] [CPD:C21529]
Product
(7Z,10Z,13Z)-hexadeca-7,10,13-trienoyl-[glycerolipid];
oxidized ferredoxin [iron-sulfur] cluster [CPD:C00139];
H2O [CPD:C00001];
alpha-linolenoyl-[glycerolipid] [CPD:C21531]
Comment
This plastidial enzyme desaturates 16:2 fatty acids attached to the sn-2 position of glycerolipids to 16:3 fatty acids, and converts18:2 to 18:3 in both the sn-1 and sn-2 positions. It acts on all 16:2- or 18:2-containing chloroplast membrane lipids, including phosphatidylglycerol, monogalactosyldiacylglycerol, digalactosyldiaclyglycerol, and sulfoquinovosyldiacylglycerol. The enzyme introduces a cis double bond at a location 3 carbons away from the methyl end of the fatty acid. The distance from the carboxylic acid end of the molecule does not affect the location of the new double bond. cf. EC 1.14.19.25, acyl-lipid omega-3 desaturase (cytochrome b5) and EC 1.14.19.36, sn-1 acyl-lipid omega-3 desaturase (ferredoxin).
History
EC 1.14.19.35 created 2015
Orthology
K10257  acyl-lipid omega-3 desaturase
Genes
CEL: CELE_W02A2.1(fat-2) CELE_Y67H2A.8(fat-1)
CBR: CBG01790(Cbr-fat-2) CBG01791(Cbr-fat-1)
BMY: Bm1_38160
LOA: LOAG_02175
NAI: NECAME_04015
LGI: LOTGIDRAFT_116269 LOTGIDRAFT_74451
OBI: 106883679
ATH: AT2G29980(FAD3) AT3G11170(FAD7) AT5G05580(FAD8)
ALY: ARALYDRAFT_478456 ARALYDRAFT_481907 ARALYDRAFT_487353
CRB: 17881387 17889809 17891716
GRA: 105776289(FAD78-2D) 105780050(FAD78-1D) 105786551(FAD78-3D) 105791965(FAD3-2.1D) 105799158(FAD3-1D)
GMX: 100037456(FAD3.1) 100217335(FAD8-1) 100305351(FAD7-1) 100499626(GMFAD7-2) 100799517(FAD8.2) 547470(FAD3.4) 547715(FAD3A) 732540(FAD3-2b)
LJA: Lj0g3v0123589.1(Lj0g3v0123589.1) Lj1g3v2337910.1(Lj1g3v2337910.1) Lj2g3v2277520.1(Lj2g3v2277520.1)
CSV: 101209295 101223135(FAD7)
MCHA: 111023722
SLY: 100134915 544203(Fad7)
DOSA: Os03t0290300-01(Os03g0290300) Os07t0693800-01(Os07g0693800) Os11t0104400-01(Os11g0104400) Os12t0104400-01(Os12g0104400)
ATS: 109748184(LOC109748184) 109753536(LOC109753536) 109765018(LOC109765018) 109778723(LOC109778723)
CRE: CHLREDRAFT_56237(FAD7)
APRO: F751_6504
PTI: PHATRDRAFT_41570(PTD15)
SYN: sll1441(desB)
SYZ: MYO_117450(desB)
SYY: SYNGTS_1727(desB)
SYT: SYNGTI_1727(desB)
SYS: SYNPCCN_1726(desB)
SYQ: SYNPCCP_1726(desB)
SYP: SYNPCC7002_A0159(desB)
AMR: AM1_3759
MAR: MAE_34620(desB)
MPK: VL20_3142
MVZ: myaer102_09850(desB)
CYT: cce_4388
TER: Tery_4492
ANA: all1597
AVA: Ava_4210
NAZ: Aazo_3556
CALH: IJ00_05015
DOU: BMF77_00181(desA_2)
CTHE: Chro_2759
 » show all
Taxonomy
Reference
1  [PMID:8226956]
  Authors
Iba K, Gibson S, Nishiuchi T, Fuse T, Nishimura M, Arondel V, Hugly S, Somerville C
  Title
A gene encoding a chloroplast omega-3 fatty acid desaturase complements alterations in fatty acid desaturation and chloroplast copy number of the fad7 mutant of Arabidopsis thaliana.
  Journal
J Biol Chem 268:24099-105 (1993)
  Sequence
[ath:AT3G11170]
Reference
2  [PMID:12232435]
  Authors
McConn M, Hugly S, Browse J, Somerville C
  Title
A Mutation at the fad8 Locus of Arabidopsis Identifies a Second Chloroplast [omega]-3 Desaturase.
  Journal
Plant Physiol 106:1609-1614 (1994)
Reference
3  [PMID:17064923]
  Authors
Venegas-Caleron M, Muro-Pastor AM, Garces R, Martinez-Force E
  Title
Functional characterization of a plastidial omega-3 desaturase from sunflower (Helianthus annuus) in cyanobacteria.
  Journal
Plant Physiol Biochem 44:517-25 (2006)
DOI:10.1016/j.plaphy.2006.09.005
Other DBs
ExplorEnz - The Enzyme Database: 1.14.19.35
IUBMB Enzyme Nomenclature: 1.14.19.35
ExPASy - ENZYME nomenclature database: 1.14.19.35
BRENDA, the Enzyme Database: 1.14.19.35

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